Team:Uppsala University/Parts

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Latest revision as of 03:51, 27 September 2012

Team Uppsala University – iGEM 2012

Favorite Parts

Small RNA Scaffold

BBa_K864440 - J23101-Spot42:

This is the native spot42 small RNA constitutively expressed by BBa_J23101 and is useful as a template for generating a randomized library of small RNAs for downregulation of any gene of interest.

Reporter System

BBa_K864444 - RFP-linker-SYFP:

This part can be used to insert any 5’UTR of a gene of interest to screen for downregulation of this gene by artificial small RNAs. This reporter reporter system comes with a fluorescent marker that is downregulated by any small RNA targeting the 5'UTR of the gene of interest.

New low-copy backbone

BBa_K864001 - pSB4C15:

This is a new BioBrick standard vector with low copy pSC101 replication origin, especially usable for Lambda Red recombineering in E coli.

Blue Chromoprotein

BBa_K864401 - aeBlue:

aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family.

Submitted Parts

BioBrick ID	Type	Name	Description
BBa_K864100	Coding	SYFP2	Yellow fluorescent protein
BBa_K864101	Composite	B0032 - SYFP2	Yellow fluorescent protein
BBa_K864102	Composite	B0034 - SYFP2	Yellow fluorescent protein
BBa_K864150	Composite	cat-sacB	Selection/counter-selection casette
BBa_K864200	Coding	MarR	Multi-resistant repressor
BBa_K864201	Coding	LexA	Repressor of the E. coli SOS-system
BBa_K864202	Coding	MarR(G95S)	Point-mutated for higher repression
BBa_K864203	Coding	LexA(G85D)	Point-mutated for higher repression
BBa_K864204	Coding	OmpF	Outer membrane porin
BBa_K864680	Coding	TetM	Ribosome protection protein
BBa_K592012	Coding	eforRed	Red/pink chromoprotein
BBa_K864401	Coding	aeBlue	Blue chromoprotein
BBa_K864402	Composite	J23110 - B0034 - eforRed	Red/pink chromoprotein
BBa_K864403	Composite	J23110 - B0034 - aeBlue	Blue chromoprotein
BBa_K864404	Composite	J23110 - B0034 - cjBlue	Turquoise chromoprotein
BBa_K864440	Scaffold	J23101 - Spot42	Scaffold for sRNAs
BBa_K864444	Measurement	RFP - linker - SYFP2	Screening system for sRNAs using a fluorescent marker
BBa_K592010	Coding	amilGFP	Yellow chromoprotein
BBa_K864600	Regulatory	T0	Synthetic terminator
BBa_K864601	Regulatory	TλT1	Synthetic terminator

Submitted backbones

BioBrick ID	Name	Description
BBa_K864000	pSB4A15	Low copy BioBrick standard vector
BBa_K864001	pSB4C15	Low copy BioBrick standard vector
BBa_K864002	pSB4K15	Low copy BioBrick standard vector
BBa_K864003	pSB4S15	Low copy BioBrick standard vector
BBa_K864004	pSB4A15(FRT)	Low copy BioBrick standard vector
BBa_K864005	pSB4C15(FRT)	Low copy BioBrick standard vector
BBa_K864006	pSB4K15(FRT)	Low copy BioBrick standard vector
BBa_K864007	pSB4S15(FRT)	Low copy BioBrick standard vector
BBa_K864008	pSB4A15iq	Low copy BioBrick standard vector with LacI
BBa_K864009	pSB4C15iq	Low copy BioBrick standard vector with LacI
BBa_K864010	pSB4K15iq	Low copy BioBrick standard vector with LacI
BBa_K864011	pSB4S15iq	Low copy BioBrick standard vector with LacI
BBa_K864013	pSB4C15iq(FRT)	Low copy BioBrick standard vector with LacI
BBa_K864014	pSB4K15iq(FRT)	Low copy BioBrick standard vector with LacI
BBa_K864017	pSB8C15	Low copy BioBrick temperature sensitive standard vector
BBa_K864019	pSB8A15	Low copy BioBrick temperature sensitive standard vector
BBa_K864020	pSB8S15	Low copy BioBrick temperature sensitive standard vector

@@ Line 36: / Line 36: @@
 <tr>
-<td valign="top">
+<td>
+<table>
 <p>
+<h3><b>Small RNA Scaffold</b></h3>
+<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864440" style="margin-left:10px">BBa_K864440</a> - <b>J23101-Spot42</b>:
+<p>This is the native spot42 small RNA constitutively expressed by BBa_J23101 and is useful as a template for generating a randomized library of small RNAs for downregulation of any gene of interest.</p>
+<br>
+<h3><b>Reporter System</b></h3>
+<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864444" style="margin-left:10px">BBa_K864444</a> - <b>RFP-linker-SYFP</b>:
+<p>This part can be used to insert any 5’UTR of a gene of interest to screen for downregulation of this gene by artificial small RNAs. This reporter reporter system comes with a fluorescent marker that is downregulated by any small RNA targeting the 5'UTR of the gene of interest.</p>
+<br>
+<h3><b>New low-copy backbone</b></h3>
+<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864001" style="margin-left:10px">BBa_K864001</a> - <b>pSB4C15</b>:
+<p>This is a new BioBrick standard vector with low copy pSC101 replication origin, especially usable for Lambda Red recombineering in E coli.</p>
+<br>
+<h3><b>Blue Chromoprotein</b></h3>
+<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864401" style="margin-left:10px">BBa_K864401</a> - <b>aeBlue</b>:
+<p>aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family.</p>
+<br>
 </p>
+</table>
+</td>
+</tr>
 <tr>
@@ Line 158: / Line 185: @@
 <td>Composite</td>
 <td>J23110 - B0034 - cjBlue</td>
-<td>Teal chromoprotein</td>
+<td>Turquoise chromoprotein</td>
 </tr>
 <tr>
@@ Line 223: / Line 250: @@
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864000">BBa_K864000</a></td>
 <td>pSB4A15</td>
-<td></td>
+<td>Low copy BioBrick standard vector</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864001">BBa_K864001</a></td>
 <td>pSB4C15</td>
-<td></td>
+<td>Low copy BioBrick standard vector</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864002">BBa_K864002</a></td>
 <td>pSB4K15</td>
-<td></td>
+<td>Low copy BioBrick standard vector</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864003">BBa_K864003</a></td>
 <td>pSB4S15</td>
-<td></td>
+<td>Low copy BioBrick standard vector</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864004">BBa_K864004</a></td>
 <td>pSB4A15(FRT)</td>
-<td></td>
+<td>Low copy BioBrick standard vector</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864005">BBa_K864005</a></td>
 <td>pSB4C15(FRT)</td>
-<td></td>
+<td>Low copy BioBrick standard vector</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864006">BBa_K864006</a></td>
 <td>pSB4K15(FRT)</td>
-<td></td>
+<td>Low copy BioBrick standard vector</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864007">BBa_K864007</a></td>
 <td>pSB4S15(FRT)</td>
-<td></td>
+<td>Low copy BioBrick standard vector</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864008">BBa_K864008</a></td>
 <td>pSB4A15iq</td>
-<td></td>
+<td>Low copy BioBrick standard vector with LacI</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864009">BBa_K864009</a></td>
 <td>pSB4C15iq</td>
-<td></td>
+<td>Low copy BioBrick standard vector with LacI</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864010">BBa_K864010</a></td>
 <td>pSB4K15iq</td>
-<td></td>
+<td>Low copy BioBrick standard vector with LacI</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864011">BBa_K864011</a></td>
 <td>pSB4S15iq</td>
-<td></td>
+<td>Low copy BioBrick standard vector with LacI</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864013">BBa_K864013</a></td>
 <td>pSB4C15iq(FRT)</td>
-<td></td>
+<td>Low copy BioBrick standard vector with LacI</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864014">BBa_K864014</a></td>
 <td>pSB4K15iq(FRT)</td>
-<td></td>
+<td>Low copy BioBrick standard vector with LacI</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864017">BBa_K864017</a></td>
 <td>pSB8C15</td>
-<td></td>
+<td>Low copy BioBrick temperature sensitive standard vector</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864019">BBa_K864019</a></td>
 <td>pSB8A15</td>
-<td></td>
+<td>Low copy BioBrick temperature sensitive standard vector</td>
 </tr>
 <tr>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864020">BBa_K864020</a></td>
 <td>pSB8S15</td>
-<td></td>
+<td>Low copy BioBrick temperature sensitive standard vector</td>
 </tr>
 </tbody>