From 2012.igem.org
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| - | Write description
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| - | ==June==
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| - | ===Week===
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| - | ====June 29th, Friday====
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| - | * [[Vent PCR]] at 11:00 (DPW)
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| - | ** Dylan's magic triple anneal method (55/60/63)
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| - | * Gel purified PCR product (DPW)
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| - | ** Quantified product at 22.4 ng/uL
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| - | ** Set up digestion of PCR product with EcoRI-HF and AscI.
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| - | *** 22 ng/uL --> 45.5 uL sample for 1 ug digest
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| - | *** Buffer 4
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| - | ** Ran digestion on gel.
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| - | * [[Miniprep]]ped overnight cultures of PL14-PL20 (STC)
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| - | ** Using C1015 rotor, 6666 x g, the Corning culture tubes only fit in the centrifuge with the lids off
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| - | * Made [[LB]], 3x 60 mL in 100 mL bottles (SS)
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| - | * Made [[LB|LB Agar]], 4 x 250 mL LB Agar in 500 mL bottles (CS)
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| - | * [[Autoclave]]d LB, LB Agar, and milliQ (SS)
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| - | * Made [[LB]] plates with Kan (CMR)
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| - | * [[Phusion PCR]] at 10:00 pm (DPW)
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| - | ** Dylan's magic triple anneal method (57/65/70, 35 cycles total)
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| - | ** Amplifying nah operon from Gibson 1
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| - | ** Appending BioBrick cutsites for ligation into pSB3C5
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Latest revision as of 16:24, 26 October 2012
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