Team:Cornell/Notebook/Salicylate reporter

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Write description
 
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==June==
 
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===Week===
 
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====June 29th, Friday====
 
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* [[Vent PCR]] at 11:00 (DPW)
 
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** Dylan's magic triple anneal method (55/60/63)
 
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* Gel purified PCR product.
 
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* [[Miniprep]]ped (STC) overnight cultures of PL0014-PL0020
 
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** Using C1015 rotor, 6666 x g, the Corning culture tubes only fit in the centrifuge with the lids off
 
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* Made [[LB]], 3x 60 mL in 100 mL bottles (SS)
 
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* Made [[LB|LB Agar]], 4 x 250 mL LB Agar in 500 mL bottles (CS)
 
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* [[Autoclave]]d LB, LB Agar, and milliQ (ss)
 

Latest revision as of 16:24, 26 October 2012

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