Team:Colombia/Modeling/Results

From 2012.igem.org

(Difference between revisions)
(Rust:)
 
(63 intermediate revisions not shown)
Line 5: Line 5:
== Results ==
== Results ==
-
The mathematical model should help the experimental design to optimize the circuit and our case was not the exception. The picture below shows the original design of the circuit.
 
 +
<p align="justify">
-
[[File:siold.png|250px|left]]
 
 +
The mathematical model should help the experimental design to optimize the circuit. This case was not the exception. The figure below shows the original design of the circuit.
-
[[File:gen2old.png|250px|center]]
+
<html>
 +
<br>
 +
</br>
 +
</html>
 +
[[File:toget.png|600px|thumb|center|Figure 1. Our desing before modeling results]]
-
After doing the simulation for the differential equations we have to make little changes to the proposed system:
+
<html>
 +
<br>
 +
</br>
 +
</html>
-
#As you can see there was an unknown promoter for LuxR. First we decided that it was a constitutive promoter but the response curves of the system were not consistent with the reality (LuxR had giant concentrations and the salicylic acid never went up). We wanted this protein to interact with LuxI and turn on the response, so we thought that it may need to be in a similar concentration of LuxI. Then we put it under the same promoter, hence the two proteins will be promoted at the same time.
 
-
#Our desired response is the increase of Salicylic Acid when a pest gets near the bacteria. With the original system the salicylic acid increased but not as much as we wanted, so to achieve this goal we tried putting the promoter activated by Lux next to the CI promoter to see if there was an increase. After running the simulation we discovered that this solution optimized the increase of salicylic acid in the response.
 
-
#Looking for the right set of parameters we came to the conclusion that the hill constant k (Concentration of the substrate when the the rate of production is half of the maximum production rate) for the promoter activated by Lux '''had''' to be 4 times greater than the hill constant for the CI promoter. Which has biological sense; the Lux promoter came from a quorum sensing system, so it needs high concentration of activator because it informs the promoter that there is high cell density. On the other hand the CI promoter box came from a bacteriophage and is used to attack the bacteria as quickly as possible, so it does need small quantities of protein to fully activate its system.
 
-
The new system is showed in the picture below:  
+
Once results from simulations were obtained ,we had to make little changes to the proposed system:  
 +
1. As it is shown, before results of simulations,  there was an unknown promoter for LuxR. In the beginning, it was established that this promoter must had been constitutive. From simulations results  we conclude that response curves of the system were not consistent with the reality (LuxR had giant concentrations and the salicylic acid never went up). In the designed system, LuxR had to interact with LuxI and turn on the response. Thus, it was thought that LuxR must had been in a similar concentration of LuxI. Consequently, LuxI was putted under the same promoter of LuxR, hence the two proteins will be promoted at the same time.
-
[[File:si.png|500px|left]]
+
<html>
 +
<br>
 +
</br>
 +
</html>
 +
2. The desired response is to increase Salicylic Acid levels when a pest gets near the bacteria. With the original system, salicylic acid increased but not as much as required. Thus, we tried putting the promoter activated by Lux next to the CI promoter in order to see if an increase was observed. With results of simulations, it was discovered that this solution optimized the increase of salicylic acid in the response.
-
[[File:gen2.png|250px|center]]
+
<html>
 +
<br>
 +
</br>
 +
</html>
 +
3. Looking for the right set of parameters, it was possible conclude that Hill constant "k" (Concentration of the substrate when the rate of production is half of the maximum production rate) for the promoter activated by Lux '''had''' to be four times greater than the Hill constant for CI promoter. This results are based on biological reasons; the Lux promoter came from a quorum sensing system, then it needs high concentration of activator. Biologically, this is  because it informs the promoter that there is high cell density. On the other hand, the CI promoter box came from a bacteriophage and it is used to attack the bacteria as quickly as possible, then it needs small quantities of protein to fully activate this system.
 +
 +
<html>
<br>
<br>
 +
</br>
 +
</html>
 +
 +
The new system is showed in the figure below:
 +
 +
<html>
<br>
<br>
 +
</br>
 +
</html>
 +
[[File:modelocorr.jpg|thumb|500px|center|Figure 2. Detection module located in the plasmid I]]
 +
<html>
 +
<br>
 +
</br>
 +
</html>
 +
[[File:p2corr.png|250px|center|thumb|Figure 3. Alert system and toxin/antitoxin system located in the plasmid II ]]
 +
</p>
-
 
+
<br>
 +
<br>
== Differential equations results ==
== Differential equations results ==
 +
<p align="justify">
Although the screening of the parameters could not be completely done, we made a manual search for them and found a set that makes the system behave as expected. Here we present the mean response of all the substances in our biological system for one cell. The impulse of the pest was made during the times 10-20.  
Although the screening of the parameters could not be completely done, we made a manual search for them and found a set that makes the system behave as expected. Here we present the mean response of all the substances in our biological system for one cell. The impulse of the pest was made during the times 10-20.  
-
'''Ralstonia'''
 
-
 
-
As you see below, when the system is under the presence of 3-OH-PAME the sensor is phosphorylated really fast and the complex phcR-phcA liberates the activator which has a peak and the goes a little down because is with the promoter and its not free. After the impulse is gone everything goes back to normality.
 
-
 
-
 
-
[[File:Ral1.png|left|450x450pxpx]] [[File: ral2.png|right|450x450pxpx]]
 
 +
<html>
<br>
<br>
-
<br>
+
</br>
-
<br>
+
</html>
-
<br>
+
-
<br>
 
<br>
<br>
-
<br>
+
===='''Ralstonia:'''====
 +
As it is shown below, when the system is under the presence of 3-OH-PAME the sensor is quickly phosphorylated and the complex phcR-phcA liberates the activator. This activator has a peak and then decrease softly because it is bound with the promoter. Once the impulse is gone, everything goes back to normality.
 +
<html>
 +
<br>
 +
</br>
 +
</html>
 +
[[File:Ral1.png|center|thumb|450x450pxpx|Figure 4. Behavior of the sensor phcS ]]
 +
[[File: ral2.png|center|thumb|450x450pxpx|Figure 5. Behavior of the activator response ]]
 +
<br>
 +
The LuxI- LuxR system increases its activations by phcsA
 +
[[File: ral3.png|center|thumb|450x450pxpx|Figure 6. Behavior of the LuxI - LuxR response ]]
 +
Looking the behavior of the species of interest, it possible to conclude that the antitoxin has greater concentration than the toxin when the 3-OH-PAME appears.  This means that the cell is awake and can produce proteins. On the other hand, the Salicylic Acid has an increase of almost two times than observed before.
-
 
+
[[File:Ral4.png|center|thumb|450x450pxpx|Figure 7. Toxin/Antitoxin response]] [[File: ral5.png|center|thumb|450x450pxpx|Figure 8. CI and Salycilic Acid response ]]
-
 
+
-
 
+
-
The LuxI- LuxR System shows and increase after it is activated by phcsA
+
-
 
+
-
[[File: ral3.png|center|450x450pxpx]]
+
-
 
+
-
Now, looking at the species of interest, we can see how the antitoxin has greater concentration than the toxin when the 3-OH-PAME appears, this means that the cell is awake and can produce proteins. On the other hand, the Salicylic acid has an increase of almost two fold.
+
-
 
+
-
[[File:Ral4.png|left|450x450pxpx]] [[File: ral5.png|right|450x450pxpx]]
+
<br>
<br>
 +
===='''Rust:'''====
 +
When the system is under the presence of chitin, it possible to see how the positive feedback of the chitoporin and chitinase works making their concentration increase. Consequently, it results in level increase of chitin monomers and release of the sensor which is going to activate the LuxI and LuxR promoter.
 +
[[File: rust1.png|center|450x450pxpx|thumb|Figure 9. Detection system substances]]
 +
Like it was shown in Ralstonia system, the Lux system concentrations increase when their promoter is activated. The complex LuxI-LuxR has a peak and then decrease because it is delay activation of CI and Salicylic Acid.
 +
[[File: rust2.png|center|thumb|450x450pxpx|Figure 10. Lux I - LuxR system substances]]
 +
The substances of interest behave just like expected. As in Ralstonia, the cell is awake when the chitin is present and the Salicylic Acid has folded its level of concentration. It is important to conclude that this system takes more time to go back to the steady state than Ralstonia's system.
 +
[[File:Rust4.png|center|thumb|450x450pxpx|Figure 11. Toxin/Antitoxin module substances]] [[File: rust5.png|center|thumb|450x450pxpx|Figure 12. CI and Salycilic Acid response]]
-
 
+
</p>
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
'''Rust:'''
+
-
 
+
-
When the system is under the presence of chitin you can see how the positive feedback of the chitoporin and chitinase works and make their concentration increase. This has as a consequence the increase of monomers of chitin and the liberation of the sensor that is gonna activate the LuxI and LuxR promoter.
+
-
 
+
-
[[File: rust1.png|center|450x450pxpx]]
+
-
 
+
-
Like we see with the Ralstonia systema the Lux system's concentrations increase when their promoter is activated. The complex LuxI-LuxR has a peak and then goes down a little because it is busy activation of CI and Salicylic Acid.
+
-
 
+
-
[[File: rust2.png|center|450x450pxpx]]
+
-
 
+
-
The compounds od interest behave just like expected. Like in Ralstonia the cell is awake when the chitin is present and the Salicylic Acid has a two fold increase. It is important to see that this system takes more time to go back to the steady state than the Ralstonia's system.
+
-
 
+
-
[[File:Rust4.png|left|450x450pxpx]] [[File: rust5.png|right|450x450pxpx]]
+
</div>
</div>

Latest revision as of 03:33, 27 October 2012

Template:Https://2012.igem.org/User:Tabima

Contents

Results

The mathematical model should help the experimental design to optimize the circuit. This case was not the exception. The figure below shows the original design of the circuit.

Figure 1. Our desing before modeling results




Once results from simulations were obtained ,we had to make little changes to the proposed system:


1. As it is shown, before results of simulations, there was an unknown promoter for LuxR. In the beginning, it was established that this promoter must had been constitutive. From simulations results we conclude that response curves of the system were not consistent with the reality (LuxR had giant concentrations and the salicylic acid never went up). In the designed system, LuxR had to interact with LuxI and turn on the response. Thus, it was thought that LuxR must had been in a similar concentration of LuxI. Consequently, LuxI was putted under the same promoter of LuxR, hence the two proteins will be promoted at the same time.



2. The desired response is to increase Salicylic Acid levels when a pest gets near the bacteria. With the original system, salicylic acid increased but not as much as required. Thus, we tried putting the promoter activated by Lux next to the CI promoter in order to see if an increase was observed. With results of simulations, it was discovered that this solution optimized the increase of salicylic acid in the response.



3. Looking for the right set of parameters, it was possible conclude that Hill constant "k" (Concentration of the substrate when the rate of production is half of the maximum production rate) for the promoter activated by Lux had to be four times greater than the Hill constant for CI promoter. This results are based on biological reasons; the Lux promoter came from a quorum sensing system, then it needs high concentration of activator. Biologically, this is because it informs the promoter that there is high cell density. On the other hand, the CI promoter box came from a bacteriophage and it is used to attack the bacteria as quickly as possible, then it needs small quantities of protein to fully activate this system.



The new system is showed in the figure below:



Figure 2. Detection module located in the plasmid I




Figure 3. Alert system and toxin/antitoxin system located in the plasmid II



Differential equations results

Although the screening of the parameters could not be completely done, we made a manual search for them and found a set that makes the system behave as expected. Here we present the mean response of all the substances in our biological system for one cell. The impulse of the pest was made during the times 10-20.


Ralstonia:

As it is shown below, when the system is under the presence of 3-OH-PAME the sensor is quickly phosphorylated and the complex phcR-phcA liberates the activator. This activator has a peak and then decrease softly because it is bound with the promoter. Once the impulse is gone, everything goes back to normality.




Figure 4. Behavior of the sensor phcS
Figure 5. Behavior of the activator response



The LuxI- LuxR system increases its activations by phcsA


Figure 6. Behavior of the LuxI - LuxR response

Looking the behavior of the species of interest, it possible to conclude that the antitoxin has greater concentration than the toxin when the 3-OH-PAME appears. This means that the cell is awake and can produce proteins. On the other hand, the Salicylic Acid has an increase of almost two times than observed before.

Figure 7. Toxin/Antitoxin response
Figure 8. CI and Salycilic Acid response


Rust:

When the system is under the presence of chitin, it possible to see how the positive feedback of the chitoporin and chitinase works making their concentration increase. Consequently, it results in level increase of chitin monomers and release of the sensor which is going to activate the LuxI and LuxR promoter.

Figure 9. Detection system substances

Like it was shown in Ralstonia system, the Lux system concentrations increase when their promoter is activated. The complex LuxI-LuxR has a peak and then decrease because it is delay activation of CI and Salicylic Acid.

Figure 10. Lux I - LuxR system substances

The substances of interest behave just like expected. As in Ralstonia, the cell is awake when the chitin is present and the Salicylic Acid has folded its level of concentration. It is important to conclude that this system takes more time to go back to the steady state than Ralstonia's system.

Figure 11. Toxin/Antitoxin module substances
Figure 12. CI and Salycilic Acid response