Team:KAIT Japan/Notebook

From 2012.igem.org

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__NOTOC__
__NOTOC__
='''Notebook'''=
='''Notebook'''=
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:Our team pushed forward an experiment while making trial and error every day.
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:We made an experiment every day. It was process of trial and error.
=='''Creating parts of Methylation and Azurin'''==
=='''Creating parts of Methylation and Azurin'''==
:<big>'''Date:8/9'''</big> PCR
:<big>'''Date:8/9'''</big> PCR
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:<big>'''Date:9/20'''</big> Ligation
:<big>'''Date:9/20'''</big> Ligation
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:<big>'''Date:9/22'''</big> Transformation
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:<big>'''Date:9/23'''</big> PCR and Electrophoresis
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:<big>'''Date:9/24'''</big> Ligation
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:<big>'''Date:9/25'''</big> Transformation
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:<big>'''Date:9/27'''</big> Miniprep and Electrophoresis
=='''Creating parts of Tar'''==
=='''Creating parts of Tar'''==

Latest revision as of 22:12, 26 September 2012

KAIT Japan2012 logo.png
KAIT Japan2012 Sub.png
Kaitjapan iGEM official.logo.png


Kaitjapan.home.png

Home

Kaitjapan project.png

Project

Kaitjapan parts.png

Parts

Kaitjapan protocol.png

Protocol

Kaitjapan notebook.png

Notebook

Kaitjapan results.png

Results

Kaitjapan safety.png

Safety

Kaitjapan human practice.png

Human Practice

Kaitjapan team.png

Team

Notebook

We made an experiment every day. It was process of trial and error.

Creating parts of Methylation and Azurin

Date:8/9 PCR
Date:8/10 Electrophoresis
Date:8/13 Electrophoresis and The refinement of DNA,PCR
Date:8/14 Transformation
Date:8/16 Blue white selection and Miniprep,Colony PCR
Date:8/17 Electrophoresis and Colony PCR
Date:8/18 PCR and Electrophoresis
Date:8/20 Extraction and the refinement of DNA
Date:8/21 PCR and Electrophoresis,Transformation
Date:8/22 Electrophoresis
Date:8/23 PCR
Date:8/25 Miniprep and Restriction enzyme processing
Date:8/27 Electrophoresis
Date:8/28 Electrophoresis and The refinement of DNA,PCR
Date:8/29 Electrophoresis
Date:8/30 Electrophoresis and The refinement of DNA
Date:9/1 Extraction of DNA
Date:9/2 Redesign of the primer
Date:9/3 PCR and Restriction enzyme processing,Electrophoresis
Date:9/5 Miniprep
Date:9/6 Electrophoresis
Date:9/10 Colony PCR
Date:9/12 Miniprep
Date:9/14 PCR
Date:9/15 Restriction enzyme processing
Date:9/16 PCR
Date:9/17 PCR
Date:9/19 PCR and Restriction enzyme processing
Date:9/20 Ligation
Date:9/22 Transformation
Date:9/23 PCR and Electrophoresis
Date:9/24 Ligation
Date:9/25 Transformation
Date:9/27 Miniprep and Electrophoresis

Creating parts of Tar

Date:9/13 PCR and Colony PCR
Date:9/14 Electrophoretic confirmation
Date:9/15 Restriction enzyme processing
Date:9/16 Electrophoresis of the restriction enzyme processing product and Restriction enzyme processing
Date:9/17 Refinement of DNA and Ligation,Transformation
Date:9/18 Making of a master plate and Preculture,Recultivation
Date:9/19 Colony PCR and Electrophoresis
Date:9/20 Ligation and Transformation
Date:9/21 Inoculation
Date:9/22 Making of a master plate and Colony PCR,Electrophoresis,Preculture
Date:9/23 Electrophoretic confirmation and Electrophoresis of the PCR
Date:9/24 Miniprep and Electrophoresis
Date:9/25 PCR of the miniprep product and Electrophoresis

Creating parts of EnvZ

Date:9/10 Design of the primer and PCR,Elecrtophoresis,Miniprep
Date:9/13 PCR and Elecrtophoresis
Date:9/18 Design of the primer
Date:9/19 PCR and Elecrtophoresis
Date:9/20 Restriction enzyme processing
Date:9/21 Ligation and PCR,Elecrtophoresis
Date:9/23 PCR
Date:9/24 The refinement of DNA and Restriction enzyme processing,Ligation
Date:9/25 Transformation
Date:9/26 Liquid culture
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