Notebook

We made an experiment every day. It was process of trial and error.

Creating parts of Methylation and Azurin

Date:8/9 PCR

Date:8/10 Electrophoresis

Date:8/13 Electrophoresis and The refinement of DNA,PCR

Date:8/14 Transformation

Date:8/16 Blue white selection and Miniprep,Colony PCR

Date:8/17 Electrophoresis and Colony PCR

Date:8/18 PCR and Electrophoresis

Date:8/20 Extraction and the refinement of DNA

Date:8/21 PCR and Electrophoresis,Transformation

Date:8/22 Electrophoresis

Date:8/23 PCR

Date:8/25 Miniprep and Restriction enzyme processing

Date:8/27 Electrophoresis

Date:8/28 Electrophoresis and The refinement of DNA,PCR

Date:8/29 Electrophoresis

Date:8/30 Electrophoresis and The refinement of DNA

Date:9/1 Extraction of DNA

Date:9/2 Redesign of the primer

Date:9/3 PCR and Restriction enzyme processing,Electrophoresis

Date:9/5 Miniprep

Date:9/6 Electrophoresis

Date:9/10 Colony PCR

Date:9/12 Miniprep

Date:9/14 PCR

Date:9/15 Restriction enzyme processing

Date:9/16 PCR

Date:9/17 PCR

Date:9/19 PCR and Restriction enzyme processing

Date:9/20 Ligation

Date:9/22 Transformation

Date:9/23 PCR and Electrophoresis

Date:9/24 Ligation

Date:9/25 Transformation

Date:9/27 Miniprep and Electrophoresis

Creating parts of Tar

Date:9/13 PCR and Colony PCR

Date:9/14 Electrophoretic confirmation

Date:9/15 Restriction enzyme processing

Date:9/16 Electrophoresis of the restriction enzyme processing product and Restriction enzyme processing

Date:9/17 Refinement of DNA and Ligation,Transformation

Date:9/18 Making of a master plate and Preculture,Recultivation

Date:9/19 Colony PCR and Electrophoresis

Date:9/20 Ligation and Transformation

Date:9/21 Inoculation

Date:9/22 Making of a master plate and Colony PCR,Electrophoresis,Preculture

Date:9/23 Electrophoretic confirmation and Electrophoresis of the PCR

Date:9/24 Miniprep and Electrophoresis

Date:9/25 PCR of the miniprep product and Electrophoresis

Creating parts of EnvZ

Date:9/10 Design of the primer and PCR,Elecrtophoresis,Miniprep

Date:9/13 PCR and Elecrtophoresis

Date:9/18 Design of the primer

Date:9/19 PCR and Elecrtophoresis

Date:9/20 Restriction enzyme processing

Date:9/21 Ligation and PCR,Elecrtophoresis

Date:9/23 PCR

Date:9/24 The refinement of DNA and Restriction enzyme processing,Ligation

Date:9/25 Transformation

Date:9/26 Liquid culture