Team:Caltech/Notebook/Biofuel

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==='''Biofuel Notebook'''===
 
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<font size="+2"><a name="6_20_12">6/20/12</a></font>
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<h1>Biofuel Notebook</h1>
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This is what we did today.
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It was awesome.  I like sheep.
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Write a paragraph here. Add an image like this:
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<div style="width:image width px; font-size:80%;"><img src="https://static.igem.org/mediawiki/2012/d/d4/Daisy.jpg" alt="alternate text" width="width" height="height" style="padding-bottom:0.5em;" /><br>This is my caption</div>
 
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La la la, here is more text.
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<a name="6_28_12"></a>
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<h3>6/28/12</h3>
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="6_21_12">6/21/12</a></font>
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We ran a test of the <a href="https://2012.igem.org/Team:Caltech/Notebook/material/Biofuel_Protocols#Ethanol_Assay"> Ethanol Assay</a> to determine its range of effectiveness.
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<font size="+2"><a name="6_22_12">6/22/12</a></font>
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Here is an example of an image without a caption:
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We ordered materials for the ethanol assay and set up experiments to grow cells anaerobically to see if ethanol could be detected.
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<img src="https://static.igem.org/mediawiki/2012/d/d4/Daisy.jpg" alt="Figure title"/>  
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="7_9_12">7/9/12</a></font>
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<font size="+2"><a name="6_23_12">6/23/12</a></font>
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We started an aerobic and an <a href="https://2012.igem.org/Team:Caltech/Notebook/material/Biofuel_Protocols#Anaerobic_Assay"> anaerobic liquid culture </a> of E. coli cells so that we could measure ethanol secreted the next day.
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="6_24_12">6/24/12</a></font>
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<font size="+2"><a name="7_10_12">7/10/12</a></font>
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We used a plate reader to measure the ethanol concentrations but found both anaerobic and aerobic cultures produced elevated ethanol levels.  This could have been because the cells grew too quickly and many were forced into anaerobic conditions in the aerobic culture.  The OD (optical density) level of the aerobic culture was elevated.
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="7_13_12">7/13/12</a></font>
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<font size="+2"><a name="6_25_12">6/25/12</a></font>
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We decided to try a few new ways to decrease aerobic ethanol yields and decided to try the experiment in flasks in a shaking incubator.
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="7_17_12">7/17/12</a></font>
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<font size="+2"><a name="6_26_12">6/26/12</a></font>
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We made additional <a href="https://2012.igem.org/Team:Caltech/Notebook/material/Biofuel_Protocols#MOPS">MOPS buffer</a> and then set our aerobic culture experiment to incubate in a shaker overnight.
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="7_18_12">7/18/12</a></font>
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<font size="+2"><a name="6_27_12">6/27/12</a></font>
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We ran an ethanol assay on our aerobic cultures.  We found that the cells had overgrown in the aerobic culture tubes and were likely producing ethanol due to forced anaerobic conditions.
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</p>
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="7_19_12">7/19/12</a></font>
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<font size="+2"><a name="6_28_12">6/28/12</a></font>
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We decided to do the ethanol assay again, this time in aerated flasks, in order to decrease the chance of anaerobic growth.  The cultures were put in MOPS media again.
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</p>
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="7_20_12">7/20/12</a></font>
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<font size="+2"><a name="6_29_12">6/29/12</a></font>
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Since the cultures once again showed high ethanol levels after the ethanol assay, we prepared a timepoint assay for the next day.  We put e. coli in flasks with 20 ml MOPS and then took OD measurements at 3 hour timepoints to determine when ethanol production was low.  We took timepoints at 1.30, 3.15, 6.30, and 22.15 from the start of the culture, and used two different colonies in two different flasks.  The results are below.
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</p>
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="6_30_12">6/30/12</a></font>
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<h1> this is h1 </h1>
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="7_1_12">7/1/12</a></font>
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<h2> this is h2> </h2>
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="7_2_12">7/2/12</a></font>
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<h3> this is h3 </h3>
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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<font size="+2"><a name="7_3_12">7/3/12</a></font>
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<h4> this is h4 </h4>
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Biofuel#Calendar">Back to Biofuel Notebook Calendar</a>
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Latest revision as of 18:50, 1 October 2012



Biofuel Notebook

6/28/12


We ran a test of the Ethanol Assay to determine its range of effectiveness.


Back to Biofuel Notebook Calendar
7/3/12

We ordered materials for the ethanol assay and set up experiments to grow cells anaerobically to see if ethanol could be detected.


Back to Biofuel Notebook Calendar
7/9/12

We started an aerobic and an anaerobic liquid culture of E. coli cells so that we could measure ethanol secreted the next day.


Back to Biofuel Notebook Calendar
7/10/12

We used a plate reader to measure the ethanol concentrations but found both anaerobic and aerobic cultures produced elevated ethanol levels. This could have been because the cells grew too quickly and many were forced into anaerobic conditions in the aerobic culture. The OD (optical density) level of the aerobic culture was elevated.


Back to Biofuel Notebook Calendar
7/13/12

We decided to try a few new ways to decrease aerobic ethanol yields and decided to try the experiment in flasks in a shaking incubator.


Back to Biofuel Notebook Calendar
7/17/12

We made additional MOPS buffer and then set our aerobic culture experiment to incubate in a shaker overnight.


Back to Biofuel Notebook Calendar
7/18/12

We ran an ethanol assay on our aerobic cultures. We found that the cells had overgrown in the aerobic culture tubes and were likely producing ethanol due to forced anaerobic conditions.


Back to Biofuel Notebook Calendar
7/19/12

We decided to do the ethanol assay again, this time in aerated flasks, in order to decrease the chance of anaerobic growth. The cultures were put in MOPS media again.


Back to Biofuel Notebook Calendar
7/20/12

Since the cultures once again showed high ethanol levels after the ethanol assay, we prepared a timepoint assay for the next day. We put e. coli in flasks with 20 ml MOPS and then took OD measurements at 3 hour timepoints to determine when ethanol production was low. We took timepoints at 1.30, 3.15, 6.30, and 22.15 from the start of the culture, and used two different colonies in two different flasks. The results are below.


Back to Biofuel Notebook Calendar

this is h1

this is h2>

this is h3

this is h4

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