Team:Lyon-INSA/datapage

From 2012.igem.org

(Difference between revisions)
 
(30 intermediate revisions not shown)
Line 7: Line 7:
<script>
<script>
$(document).ready(function(){
$(document).ready(function(){
 +
$("#result").click();
$(".legendePicto").cluetip({splitTitle: '|', showTitle: false});
$(".legendePicto").cluetip({splitTitle: '|', showTitle: false});
Line 14: Line 15:
<style>
<style>
.legendePicto{
.legendePicto{
-
margin-left:200px;
+
margin-left:190px;
}
}
Line 20: Line 21:
<head>
<head>
</head>
</head>
 +
<SCRIPT LANGUAGE="JavaScript">
 +
// CHARGEMENT DES IMAGES AVANT LE RESTE
 +
i1 = new Image;
 +
i1.src = "https://static.igem.org/mediawiki/2012/8/86/RepointageA.PNG";
 +
i2 = new Image;
 +
i2.src = "https://static.igem.org/mediawiki/2012/d/d8/Hover1%28bis%29.PNG";
 +
i3 = new Image;
 +
i3.src = "https://static.igem.org/mediawiki/2012/f/f2/RepointageB.PNG";
 +
i4 = new Image;
 +
i4.src = "https://static.igem.org/mediawiki/2012/f/fc/Repointage2.PNG";
 +
i5 = new Image;
 +
i5.src = "https://static.igem.org/mediawiki/2012/4/46/Pointage3bis.PNG";
 +
i6 = new Image;
 +
i6.src = "https://static.igem.org/mediawiki/2012/f/fc/RepointageC.PNG";
 +
i7 = new Image;
 +
i7.src = "https://static.igem.org/mediawiki/2012/f/fb/RepointageD.PNG";
 +
i8 = new Image;
 +
i8.src = "https://static.igem.org/mediawiki/2012/e/e1/RepointageE.PNG";
 +
i9 = new Image;
 +
i9.src = "https://static.igem.org/mediawiki/2012/b/b9/RepointageF.PNG";
 +
</script>
<body>
<body>
-
 
+
<div id="pageContent">
-
 
+
-
<div id="projectBar" class="menuBar">
+
-
    <div class="boutonMenu" onclick="window.location='menu';">Menu</div>
+
-
    <div id="ongletBar">
+
-
        <div class="boutonOnglet"  onclick="window.location='project';">Project</div>
+
-
        <div class="boutonOnglet"  onclick="window.location='modelling';">Modelling</div>
+
-
        <div class="boutonOnglet"  onclick="window.location='safety';">Safety</div>
+
-
        <div class="boutonOnglet" onclick="window.location='notebook';">Notebook</div>
+
-
        <div class="boutonOnglet"  onclick="window.location='protocol';">Protocols</div>
+
-
        <div class="boutonOnglet BOClicked" >Data Page</div>
+
-
        <div class="boutonOnglet" onclick="window.location='Achievements';">Achievements</div>
+
-
    </div>
+
-
</div>
+
-
<div id="bandeau"></div>
+
-
<br/>
+
<h1>Interactive pattern of our construction : Toggle switch option</h1>
<h1>Interactive pattern of our construction : Toggle switch option</h1>
-
<div style="margin:20px;font-size:15px;">
+
<div style="margin:20px;font-size:15px;margin-top:0px;">
<center><strong><big>Hover your mouse over a step number/letter to see more</big></strong></center><br/>
<center><strong><big>Hover your mouse over a step number/letter to see more</big></strong></center><br/>
<br/>
<br/>
<div id="schemaRecap" width="600" style="margin-left:200px">
<div id="schemaRecap" width="600" style="margin-left:200px">
<div id="picto1" class="legendePicto" title="|<img width='600' src='https://static.igem.org/mediawiki/2012/d/d8/Hover1%28bis%29.PNG'/>" style="background-color:transparent;z-index:50;width:30px;height:30px;position:absolute;left:45px;margin-top:57px"></div>
<div id="picto1" class="legendePicto" title="|<img width='600' src='https://static.igem.org/mediawiki/2012/d/d8/Hover1%28bis%29.PNG'/>" style="background-color:transparent;z-index:50;width:30px;height:30px;position:absolute;left:45px;margin-top:57px"></div>
-
<div id="pictoA" title="|<img width='600' src='https://static.igem.org/mediawiki/2012/8/86/RepointageA.PNG'/>"  class="legendePicto" style="background-color:black;z-index:50;width:30px;height:30px;position:absolute;left:200px;margin-top:130px"></div>
+
<div id="pictoA" title="|<img width='600' src='https://static.igem.org/mediawiki/2012/8/86/RepointageA.PNG'/>"  class="legendePicto" style="background-color:transparent;z-index:50;width:30px;height:30px;position:absolute;left:200px;margin-top:130px"></div>
<div id="pictoB" class="legendePicto" title="|<img width='550' src='https://static.igem.org/mediawiki/2012/f/f2/RepointageB.PNG'/>"  style="background-color:transparent;z-index:50;width:30px;height:30px;position:absolute;left:305px;margin-top:130px"></div>
<div id="pictoB" class="legendePicto" title="|<img width='550' src='https://static.igem.org/mediawiki/2012/f/f2/RepointageB.PNG'/>"  style="background-color:transparent;z-index:50;width:30px;height:30px;position:absolute;left:305px;margin-top:130px"></div>
<div id="picto2" title="|<img width='600' src='https://static.igem.org/mediawiki/2012/f/fc/Repointage2.PNG'/>"  class="legendePicto" style="background-color:transparent;z-index:50;width:30px;height:30px;position:absolute;left:70px;margin-top:270px"></div>
<div id="picto2" title="|<img width='600' src='https://static.igem.org/mediawiki/2012/f/fc/Repointage2.PNG'/>"  class="legendePicto" style="background-color:transparent;z-index:50;width:30px;height:30px;position:absolute;left:70px;margin-top:270px"></div>
Line 60: Line 67:
<div class="petitSsTitre">Data about our favorite new parts </div>
<div class="petitSsTitre">Data about our favorite new parts </div>
-
1.<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802001" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Dispersin generator for <i>B. subtilis</i></b> BBa_K802001 : This part associates the <i>Bacillus subtilis</i> Constitutive Promoter (P<sub>veg</sub>) with the dispersin B gene (<i>dspB</i>). We tried two different genetic backgrounds : the NM522 strain to make test in <i>E. coli</i> and the 168 strain to make test in <i>Bacillus subtilis</i>.<br>
+
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802001" target="_blank"><font color="#664499"><b>1. Main Page</b></font></a>: <b>Dispersin generator for <i>B. subtilis</i></b> BBa_K802001 : This part associates the <i>Bacillus subtilis</i> constitutive promoter (P<sub>veg</sub>) with the dispersin B gene (<i>dspB</i>). This part allows to efficiently scatter Staphylococci biofilms. <br>
-
<br>
+
-
2.<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802003" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b>BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>low</b> copy number plasmid in <i>B. subtilis</i> and a high copy number plasmid in <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. We determined antibiotic resistance thresholds and transformation efficiencies and compared them to BBa_K802004<br>
 
-
<br>
 
 +
<br><br>
 +
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802000" target="_blank"><font color="#664499"><b>2. Main Page</b></font></a>: <b>Lysostaphin generator for <i>B. subtilis</i></b> BBa_K802000  : This part associates the <i>Bacillus subtilis</i> constitutive promoter (P<sub>veg</sub>) with the lysostaphin gene. This part allows an efficient killing of <i>S. aureus</i> cells.<br><br>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802009" target="_blank"><font color="#664499"><b>3. Main Page</b></font></a>: <b>Surfactin generator and biofilm repressor for <i>B. subtilis</i></b> BBa_K802009: This part can be used to induce surfactin production and to repress the biofilm formation in <i> B. subtilis</i> strains (COAT module).<font color="RED"><b>NEW</b></font>
<br>
<br>
-
3.<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802000" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Lysostaphin generator for <i>B. subtilis</i></b> BBa_K802000  : This part associates the <i>Bacillus subtilis</i> Constitutive Promoter (P<sub>veg</sub>) with the lysostaphin gene. The pBKL28 plasmid was transformed in the NM522 strain to perform tests in <i>E. coli</i> and in the 168 strain to perform tests in <i>Bacillus subtilis</i>.<br>
 
<div class="petitSsTitre">We've also characterized the following new parts </div>
<div class="petitSsTitre">We've also characterized the following new parts </div>
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802002" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>P<sub>lac</sub>(<i>B. subtilis</i>)-RBS(<i>E. coli</i>)-GFP</b>  BBa_K802002 : This part is used to determine if the P<sub>lac</sub> promoter works or not and especially how it works when it is activated by different concentration of the inducer IPTG. Within this part there is P<sub>lac</sub> from <i>Bacillus subtilis</i> which is the promoter that induces production of XylR to form a positive biofilm. Then a RBS from <i>E coli</i> is added to finally enable production of GFP which is an easy measurable parameter using the amount of fluorescence.<br>
+
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802002" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>P<sub>lac</sub>-RBS-GFP</b>  BBa_K802002 : This part has been designed to determine the behavior of the P<sub>lac</sub> promoter used to drive the STICK module (<span class="unProto" onclick="window.open('http://partsregistry.org/Part:BBa_K802009', 'Part BBa_K802009'); return false;">Part BBa_K802009</span>)
 +
 
<br>
<br>
<br>
<br>
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802004" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b> BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>high</b> copy number plasmid in both <i>B. subtilis</i> and <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. We determined antibiotic resistance thresholds and transformation efficiencies and compared them to BBa_K802003<br>
+
 
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802003" target="_blank"><font color="#664499"><b> Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b>BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>low</b> copy number plasmid in <i>B. subtilis</i> and a high copy number plasmid in <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in <i>B. subtilis</i> by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.
<br>
<br>
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802009" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Surfactin generator and biofilm repressor for <i>B. subtilis</i></b> BBa_K802009: This part can be used to induce surfactin production (an antimicrobial lipopeptide) and to repress the biofilm formation in <i> B. subtilis</i> strains.
 
<br>
<br>
-
</body>
+
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802004" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b> BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>high</b> copy number plasmid in both <i>B. subtilis</i> and <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in <i>B. subtilis</i> by a transformation yield of 80 transformants/µg and a erythromycin resistance to at least 1.5 mg/mL.
 +
<br><br>
 +
 
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802007" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Biofilm repressor for <i>B. subtilis</i> strains</b> BBa_K802007 : This part can be used to impede biofilm formation in <i>B. subtilis</i>, more particularly if it is arbB-. This part was characterized in conjuction with parts K802006 and K802008.<font color="RED"><b>NEW</b></font>
 +
<br/><br/>
 +
 
</div>
</div>
 +
</div>
 +
</body>
 +
</html>
</html>
{{Lyon-INSA/pub}}
{{Lyon-INSA/pub}}

Latest revision as of 00:40, 27 October 2012

Interactive pattern of our construction : Toggle switch option

Hover your mouse over a step number/letter to see more


Our parts :

Data about our favorite new parts
1. Main Page: Dispersin generator for B. subtilis BBa_K802001 : This part associates the Bacillus subtilis constitutive promoter (Pveg) with the dispersin B gene (dspB). This part allows to efficiently scatter Staphylococci biofilms.


2. Main Page: Lysostaphin generator for B. subtilis BBa_K802000 : This part associates the Bacillus subtilis constitutive promoter (Pveg) with the lysostaphin gene. This part allows an efficient killing of S. aureus cells.

3. Main Page: Surfactin generator and biofilm repressor for B. subtilis BBa_K802009: This part can be used to induce surfactin production and to repress the biofilm formation in B. subtilis strains (COAT module).NEW
We've also characterized the following new parts
Main Page: Plac-RBS-GFP BBa_K802002 : This part has been designed to determine the behavior of the Plac promoter used to drive the STICK module (Part BBa_K802009)

Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low copy number plasmid in B. subtilis and a high copy number plasmid in E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.

Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high copy number plasmid in both B. subtilis and E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 80 transformants/µg and a erythromycin resistance to at least 1.5 mg/mL.

Main Page: Biofilm repressor for B. subtilis strains BBa_K802007 : This part can be used to impede biofilm formation in B. subtilis, more particularly if it is arbB-. This part was characterized in conjuction with parts K802006 and K802008.NEW


Retrieved from "http://2012.igem.org/Team:Lyon-INSA/datapage"