Team:Paris-Saclay/Project/Notebook/Week 5

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<a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract">
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<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div>
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<img src="https://static.igem.org/mediawiki/2012/d/d1/Project2.jpg" alt="" />
<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div>
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{{Team:Paris-Saclay/Follow}}
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='''Week 5'''=
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[[Category:Team:Paris-Saclay/Project Gemote/Notebook|e]]
[[Category:Team:Paris-Saclay/Project Gemote/Notebook|e]]
__NOTOC__
__NOTOC__
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*End of liquid culture (for the grooving colonies)
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*End of liquid culture (for the grooving colonies).
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*End of Miniprep to have usable biobricks
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*End of Miniprep : we have usable biobricks.
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*Culture stocks for each biobrick, to have a reserve of DNA
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*Culture stocks for each biobrick, to have a reserve of DNA.
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*PCR to amplify the different biobricks we will need to put together the different fragments using Gibson Assembly Method
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*PCR to amplify the different biobricks we will need to put together the different fragments using the Gibson Assembly Method.
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*Electrophoresis gel to verify the length of each biobricks
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*Electrophoresis gel to verify the length of each biobrick.
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*Purification of the PCR products if the length was the good one
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*Purification of the PCR products when we had the correct length.
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{{Team:Paris-Saclay/Follow}}
{{Team:Paris-Saclay/Follow}}

Latest revision as of 00:29, 27 September 2012

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Week 5

  • End of liquid culture (for the grooving colonies).
  • End of Miniprep : we have usable biobricks.
  • Culture stocks for each biobrick, to have a reserve of DNA.
  • PCR to amplify the different biobricks we will need to put together the different fragments using the Gibson Assembly Method.
  • Electrophoresis gel to verify the length of each biobrick.
  • Purification of the PCR products when we had the correct length.