Team:LMU-Munich/Weekly Journal

From 2012.igem.org

(Difference between revisions)
 
(13 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo9.jpg|6}}
{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo9.jpg|6}}
-
[[File:Weekly_journal_banner.jpg|620px|link=]]
+
[[File:Weekly journal banner.resized WORDS.JPG|620px|link=]]
-
 
-
==Weekly Journal==
 
<p></p>
<p></p>
Line 20: Line 18:
|[[Team:LMU-Munich/Weekly Journal#July|July]]
|[[Team:LMU-Munich/Weekly Journal#July|July]]
|[[Team:LMU-Munich/Weekly Journal#October|October]]
|[[Team:LMU-Munich/Weekly Journal#October|October]]
 +
|-
 +
|[[Team:LMU-Munich/Weekly Journal# | ]]
 +
|[[Team:LMU-Munich/Weekly Journal# | ]]
 +
|[[Team:LMU-Munich/Weekly Journal#November|November]]
|}
|}
</div>
</div>
Line 80: Line 82:
  -->
  -->
 +
<div class="box" style= "background-color:#e4f1d7">
 +
===November===
 +
</div>
 +
 +
<div class="box">
 +
'''4-5 November 2012'''
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> Boston Jamboree!!</p>
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> OMG! We were finalist! We won "best wiki" together with Slovenia and best "new application"!</p>
 +
</div>
 +
<div class="box" style= "background-color:#e4f1d7">
 +
===October===
 +
</div>
 +
 +
<div class="box">
 +
'''29 October - 3 November 2012'''
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> Flying across the Atlantic! On our way to the World Championship Jamboree in Boston!</p>
 +
</div>
 +
<div class="box">
 +
'''22-26 October 2012'''
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> Our dedicated presentation and website mini-team (Franzi, Korinna and Tamara) is working hard.</p>
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=40"/></a></html> Round two of time-lapse microscopy. We decided to try a new media combination and flow speed to see if we can prevent the cell lysis we experienced last week.</p>
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=40"/></a></html> We brain-stormed some ways to create a device for our '''Sporo'''beads. We asked our Gold Sponsor [https://2012.igem.org/Team:LMU-Munich/Sponsors Semadeni] if they would donate a few 0,20um filter spin columns to use for our envisioned sporo-device. We also batch-produced spores of our strain B53. Even with its modifications, it has definitely not lost its sporulation ability!</p>
 +
</div>
 +
<div class="box">
 +
'''15-19 October 2012'''
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> We got some great ideas from other teams at the European Jamboree about how to better present our project. We're working on a new version of our presentation for Boston.</p>
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=40"/></a></html> One of our collaborators, the [https://2012.igem.org/Team:Bielefeld-Germany Bielefeld iGEM team], graciously sent us two laccases to use on our '''Sporo'''beads.</p>
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=40"/></a></html> We wanted to demonstrate the formation of GFP-expressing spores in our cells, so we decided to try time-lapse microscopy. Our first round of time-lapse failed, as the cells spontaneously lysed after 10 hours. We were baffled by this reaction. Well, time to do some research.</p>
 +
</div>
 +
<div class="box">
 +
'''7-12 October 2012'''
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> On Sunday at the Jamboree, we got the great news that our team will take the '''Bead'''zillus project to Boston!</p>
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> Back home to Munich, and a week of much-needed rest for most of the team before heading back to the lab.</p>
 +
</div>
 +
<div class="box">
 +
'''1-6 October 2012'''
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> Working on the final touches to our poster and presentation.</p>
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> The whole team spent the entire Wednesday mixing, rolling, punching, baking and frosting between 600 and 800 '''Bead'''zillus cookies to bring to the European Jamboree poster session!</p>
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> By planes and trains, we made our way to the European Jamboree in the lovely city of Amsterdam.</p>
 +
 +
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=25"/></a></html> Friday and Saturday were the Jamboree: we gave our presentation, presented our poster, and distributed lots of '''Bead'''zillus cookies! We also met several potential collaboration partners, including [http://www.bsse.ethz.ch/bpl/people/panke Sven Panke] of [https://2012.igem.org/Team:ETH_Zurich ETH Zurich] who proposed the very interesting idea of using our '''Sporo'''beads for protein evolution.</p>
 +
</div>
<div class="box" style= "background-color:#e4f1d7">
<div class="box" style= "background-color:#e4f1d7">
===September===
===September===
Line 92: Line 149:
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=40"/></a></html>
<p align="justify"><html><a><img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=40"/></a></html>
-
Sitting on the computer every day and analyzing the sheer number of microscopy pictures...</p>
+
Sitting on the computer every day and analyzing the sheer number of microscopy pictures... Check out our [https://2012.igem.org/Team:LMU-Munich/Data/gfp_spore data]</p>
</div>
</div>
Line 146: Line 203:
<p align="justify"><html><a ">
<p align="justify"><html><a ">
<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=40"/></a></html>
<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=40"/></a></html>
-
All of our CotZ-GFP variants were examined with fluorescence microscopy! The brightest spores derived from our P<sub>''cotyz''</sub>-''cotZ''rep-''gfp''-terminator mutants.</p>
+
All of our CotZ-GFP variants were examined with fluorescence microscopy! The brightest spores derived from our B 53 strain (containing P<sub>''cotyz''</sub>-''cotZ''rep-''gfp''-terminator).</p>
<p align="justify"><html><a>
<p align="justify"><html><a>

Latest revision as of 13:37, 14 November 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo9.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Weekly_Journal"