Latest revision as of 01:42, 27 October 2012

Template:Https://2012.igem.org/User:Tabima

Parts

Toxin- Antitoxin (TA) Modules

Our Basic Parts

Our Composite Parts

We send all this parts to the Registry!!!!

[http://partsregistry.org/Part:BBa_K831000:Design HipA7] [http://partsregistry.org/Part:BBa_K831003:Design TisB] [http://partsregistry.org/Part:BBa_K831001:Design HipB] [http://partsregistry.org/Part:BBa_K831002:Design MqsA] [http://partsregistry.org/Part:BBa_K831004:Design istR ]

[http://partsregistry.org/Part:BBa_K831015 prm-hipA7] [http://partsregistry.org/Part:BBa_K831009 plac-hipB] [http://partsregistry.org/Part:BBa_K831007 ptetR-mqsR] [http://partsregistry.org/Part:BBa_K831014 plac-istR]

Ralstonia:

pchS: It encodes a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.

pchR: Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.

pchA:LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.

[http://partsregistry.org/Part:BBa_K831016 PxpsR:] Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.

[http://partsregistry.org/Part:BBa_K831017 PxpsR-eYFP:] Fluorescent Reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.

Aliivibrio fischeri

The overall model can be seen in figures in the main page of Aliivibrio/Streptomyces.

chiP: [http://www.ncbi.nlm.nih.gov/gene/3279194 Chitoporin] is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,

chiA: [http://www.ncbi.nlm.nih.gov/gene/3277358 Chitinase] is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.

chiS: [http://www.ncbi.nlm.nih.gov/gene/3279287 The histidine-kinase sensor] is part of a two-component signalling system, that activates a promoter, in this case pChitin.

cbp: [http://www.ncbi.nlm.nih.gov/gene/3279341 Chitin-binding protein] is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.

PChitin: This 100bp region upstream of chiP is thought to be activated by the activation of chiS.

Alert System:

@@ Line 1: / Line 1: @@
 {{https://2012.igem.org/User:Tabima}}
+<div class="right_box">
 == '''Parts''' ==
-== ''Toxin- Antitoxin (TA) Modules ==
+== '''Toxin- Antitoxin (TA) Modules''' ==
@@ Line 10: / Line 12: @@
-[[File:TA_BioBricks1.png|center|700px]]
+[[File:TA_BioBricks1-2.png|center|550px]]
-'''The links to our BioBricks are'''
-== Ralstonia: ==
+== Our Composite Parts ==
-[[File:ralral.png|center|500x300px]]
+[[File:TA_BioBricks3.png|center|550px]]
+='''We send all this parts to the Registry!!!! '''=
-'''pchS:'''
+[http://partsregistry.org/Part:BBa_K831000:Design HipA7]
+[http://partsregistry.org/Part:BBa_K831003:Design TisB]
+[http://partsregistry.org/Part:BBa_K831001:Design HipB]
+[http://partsregistry.org/Part:BBa_K831002:Design MqsA]
+[http://partsregistry.org/Part:BBa_K831004:Design ''istR'' ]
+[http://partsregistry.org/Part:BBa_K831015 prm-''hipA7'']
+[http://partsregistry.org/Part:BBa_K831009 plac-''hipB'']
+[http://partsregistry.org/Part:BBa_K831007 ptetR-''mqsR'']
+[http://partsregistry.org/Part:BBa_K831014  plac-''istR'']
-'''pchR:'''
+== '''Ralstonia:''' ==
-'''pchA:'''
+[[File:Ralstoniaparts.png|center|550px]]
-'''PxpcR:'''
-== Alivibrio ==
+'''pchS:''' It encodes a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.
-[[File:alirus.png|center|500x300px|]]
+'''pchR:''' Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.
-'''ChiP:'''
+'''pchA:'''LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.
-'''ChiA:'''
+[http://partsregistry.org/Part:BBa_K831016 '''PxpsR:'''] Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.
-'''ChiS:'''
+[http://partsregistry.org/Part:BBa_K831017 '''PxpsR-eYFP:'''] Fluorescent Reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.
-'''CBP:'''
+== ''Aliivibrio fischeri'' ==
-== Alert System: ==
+[[File:alis.png|center|500px|]]
-[[File:alertd.png|center|500x300px|]]
+The overall model can be seen in figures in [https://2012.igem.org/Team:Colombia/Project/Experiments/Aliivibrio_and_Streptomyces the main page of Aliivibrio/Streptomyces.]
-'''LuxI:'''
+'''chiP:''' [http://www.ncbi.nlm.nih.gov/gene/3279194 Chitoporin] is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,
-'''LuxR:'''
+'''chiA:''' [http://www.ncbi.nlm.nih.gov/gene/3277358 Chitinase] is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.
+'''chiS:''' [http://www.ncbi.nlm.nih.gov/gene/3279287 The histidine-kinase sensor] is part of a two-component signalling system, that activates a promoter, in this case pChitin.
+'''cbp:''' [http://www.ncbi.nlm.nih.gov/gene/3279341 Chitin-binding protein] is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.
+'''PChitin:''' This 100bp region upstream of chiP is thought to be activated by the activation of chiS.
+== Alert System: ==
-'''CI:'''
+[[File:Cutandpasteparts.png|center|600px]]
-'''Salicylic Acid:'''
+</div>

Team:Colombia/Parts

From 2012.igem.org