|
|
| (One intermediate revision not shown) |
| Line 263: |
Line 263: |
| | <h2>Our plant genes:</h2> | | <h2>Our plant genes:</h2> |
| | <p> | | <p> |
| - | First primer Extended Start codon Stop codon Restriction site Mutagenesis primer
| + | |
| | + | <b>1-SST:</b> |
| | + | |
| | <b>1-FFT:</b> | | <b>1-FFT:</b> |
| - | AGGAGGTGTCACCATGCAAACCCCTGAACCCTTTACAGACCTTGAACATGAACCCCACACACCCCTACTGGACCACCACCACAACCCACCACCACAA</br>
| + | |
| - | ACCACCACAAAACCTTTGTTCACCAGGGTTGTGTCCGGTGTCACCTTTGTTTTATTCTTCTTTGGTTTCGCTATCGTATTCATTGTTCTCAACCAAC</br>
| + | |
| - | AGAACTCTTCTGTTCGTATCGTCACCAATTCGGAGAAATCTTTTATAAGGTATTCGCAGACCGATCGCTTGTCGTGGGAACGGACCGCTTTTCATTT</br>
| + | |
| - | TCAGCCTGCCAAGAATTTTATTTACGATCCAGATGGTCAGTTGTTTCACATGGGCTGGTACCATATGTTCTATCAATACAACCCATACGCACCGGTT</br>
| + | |
| - | TGGGGCAATATGTCATGGGGTCACTCAGTGTCCAAAGACATGATCAACTGGTACGAGCTGCCAGTCGCTATGGTCCCGACCGAATGGTATGATATCG</br>
| + | |
| - | AGGGCGTCTTATCCGGGTCTACCACGGTCCTTCCAAACGGTCAGATCTTTGCATTGTATACTGGGAACGCTAATGATTTTTCCCAATTACAATGCAA</br>
| + | |
| - | AGCTGTACCCGTAAACTTATCTGACCCGCTTCTTATTGAGTGGGTCAAGTATGAGGATAACCCAATCCTGTACACTCCACCAGGGATTGGGTTAAAG</br>
| + | |
| - | GACTATCGGGACCCGTCAACAGTCTGGACAGGTCCCGATGGAAAGCATAGGATGATCATGGGAACTAAACGTGGCAATACAGGCATGGTACTTGTTT</br>
| + | |
| - | ACTATACCACTGATTACACGAACTACGAGTTGTTGGATGAGCCGTTGCACTCTGTTCCCAACACCGATATGTGGGAATGCGTCGACTTTTACCCGGT</br>
| + | |
| - | TTCGTTAACCAATGATAGTGCACTTGATATGGCGGCCTATGGGTCGGGTATCAAACACGTTATTAAAGAAAGTTGGGAGGGACATGGAATGGATTGG</br>
| + | |
| - | TATTCAATCGGGACATATGACGCGATAAATGATAAATGGACTCCCGATAACCCGGAACTAGATGTCGGTATCGGGTTACGGTGCGATTACGGGAGGT</br>
| + | |
| - | TTTTTGCATCAAAGAGTCTTTATGACCCATTGAAGAAAAGGAGGATCACTTGGGGTTATGTTGGAGAATCAGATAGTGCTGATCAGGACCTCTCAAG</br>
| + | |
| - | AGGATGGGCTACTGTTTATAATGTTGGAAGAACAATTGTACTAGATAGAAAGACCGGGACCCATTTACTTCATTGGCCCGTTGAGGAAGTCGAGAGT</br>
| + | |
| - | TTGAGATACAACGGTCAGGAGTTTAAAGAGATCAAGCTAGAGCCCGGTTCAATCATTCCACTCGACATAGGCACGGCTACACAGTTGGACATAGTTG</br>
| + | |
| - | CAACATTTGAGGTGGATCAAGCAGCGTTGAACGCGACAAGTGAAACCGATGATATTTATGGTTGCACCACTAGCTTAGGTGCAGCCCAAAGGGGAAG</br>
| + | |
| - | TTTGGGACCATTTGGTCTTGCGGTTCTAGCCGATGGAACCCTTTCTGAGTTAACTCCGGTTTATTTCTATATAGCTAAAAAGGCAGATGGAGGTGTG</br>
| + | |
| - | TCGACACATTTTTGTACCGATAAGCTAAGGTCATCACTAGATTATGATGGGGAGAGAGTGGTGTATGGGGGCACTGTTCCTGTGTTAGATGATGAAG</br>
| + | |
| - | AACTCACAATGAGGCTATTGGTGGATCATTCGATAGTGGAGGGGTTTGCGCAAGGAGGAAGGACGGTTATAACATCAAGGGCGTATCCAACAAAAGC</br>
| + | |
| - | GATATACGAACAAGCGAAGCTGTTCTTGTTCAACAACGCCACAGGTACGAGTGTGAAAGCATCTCTCAAGATTTGGCAAATGGCTTCTGCACCAATT</br>
| + | |
| - | CATCAATACCCTTTTTAATTACCGGCTATCGCTATCCTTTTTGTTATTG</br>
| + | |
| | | | |
| | | | |
"
