Team:LMU-Munich/Strains

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==''Bacillus'' strain collection==
 
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Because we envision an iGEM world where work with ''Bacillus subtilis'' is as easy as work with ''E. coli'', here we offer the last piece of the puzzle. Until now, there has been no possibility of exchanging ''Bacillus subtilis'' strains through the Parts Registry. However for work with ''Bacillus subtilis'', the option to exchange and work with different strains is much more important than it is with ''E. coli'' because:
 
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Because we envision an iGEM world where work with ''Bacillus subtilis'' is as easy as work with ''Escherichia coli'', we offer the last piece of the puzzle. Until now, there has been no possibility of exchanging ''Bacillus subtilis'' strains through the Parts Registry. However, for work with ''B. subtilis'', the option to exchange and work with different strains is much more important than it is with ''E. coli'' because:
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<br>
* Mostly integrative plasmids were used. Hence, the storage of the plasmids is less important than the strains.
* Mostly integrative plasmids were used. Hence, the storage of the plasmids is less important than the strains.
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* The transformation of genomic DNA in ''B. subtilis'' is much more efficient than with plasmids.
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* These genetic modifications can be easily transferred to other strains by isolation and transformation of genomic DNA
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<html> <a href="https://2012.igem.org/File:LMU-Munich-Bacillus_strain_collection.xls">
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<img src="https://static.igem.org/mediawiki/2012/9/9e/LMU-Munich-Bacillus_strain_collection_snap.png" heigth=100"></a></html>
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To facilitate an easy storage and exchange of relevant ''B. subtilis'' strains, '''the Mascher lab offers to maintain an iGEM ''Bacillus subtilis'' strain collection''' for the time being. By clicking on the picture below, you can download an Excel spread sheet summarizing all ''B. subtilis'' strains currently available in this collection. So far, this is limited to the strains constructed by the LMU-Munich iGEM team 2012 as part of their [https://2012.igem.org/Team:LMU-Munich '''Bead'''zillus] project.</p>
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Here, you can download an excel file with all available ''B. subtilis'' strains.
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Here
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<html> <a href="https://2012.igem.org/File:LMU-Munich-Bacillus_strain_collection.xls">
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<img src="https://static.igem.org/mediawiki/2012/9/9e/LMU-Munich-Bacillus_strain_collection_snap.png" width="620"></a></html>
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As long as there is no option in the Parts Registry to store and order ''Bacillus subtilis'' strains, '''the lab of Prof. Thorsten Mascher offers to maintain an iGEM ''Bacillus subtilis'' strain collection'''. This means that iGEM teams can send their strains or order available strains.
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<p align="justify">If you would like to request a strain or contribute to the ''Bacillus subtilis'' collection by providing novel strains, please contact [http://syntheticmicrobe.bio.lmu.de/members/mascher/index.html Prof. Thorsten Mascher].
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</p>

Latest revision as of 13:57, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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