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Team:Paris-Saclay/Project/Notebook/Week 4
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| - | + | <a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract"> | |
| - | + | <div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | |
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| - | + | ='''Week 4'''= | |
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[[Category:Team:Paris-Saclay/Project Gemote/Notebook|d]] | [[Category:Team:Paris-Saclay/Project Gemote/Notebook|d]] | ||
__NOTOC__ | __NOTOC__ | ||
| + | *We placed an order for the primers. | ||
| + | *DNA (biobricks) Resuspension using iGEM protocol. | ||
| + | *Thermal shock treatment of each biobrick to have a large quantity of DNA. | ||
| + | *Staggering of the transformed bacteria on a selective medium to select bacteria which recieved the plasmid. | ||
| + | *We started some colonies, one per biobrick, on a liquid culture, in order to make a Miniprep. | ||
| + | *Miniprep to purify each biobrick (ready to use). | ||
| + | *Groove for two colonies because they did not grow on the liquid culture. | ||
{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
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Latest revision as of 00:29, 27 September 2012
Week 4
- We placed an order for the primers.
- DNA (biobricks) Resuspension using iGEM protocol.
- Thermal shock treatment of each biobrick to have a large quantity of DNA.
- Staggering of the transformed bacteria on a selective medium to select bacteria which recieved the plasmid.
- We started some colonies, one per biobrick, on a liquid culture, in order to make a Miniprep.
- Miniprep to purify each biobrick (ready to use).
- Groove for two colonies because they did not grow on the liquid culture.
Follow us !