Team:Peking

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Latest revision as of 20:16, 26 October 2012

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L

ight has created innumerable wonders in nature. Equipped with tools and methods in optogenetic research, scientists have also achieved some truly fascinating goals in a synthetic way. This year, 2012 Peking-ECUST iGEM utilized an ultra-sensitive engineered sensor of luminescence -- the Luminesensor. Amazingly, the Luminesensor was proven to be so sensitive as to be able to detect natural light and even bioluminescence. With this sensor, spatiotemporal control of cellular behavior, such as high-resolution 2D and 3D bio-printing using dim light and even the luminescence of iPad were shown to be very easy. What’s more, we successfully implemented cell-cell signaling using light for the very first time in synthetic biology, which will be of great importance for biotechnological use.

Abstract

Luminesensor

Strongly motivated to open up a new era of optogenetics, our team has utilized an ultra-sensitive sensor which is engineered for sensing luminance – named by us Luminesensor, enabling highly efficient spatiotemporal control of biochemical process or cellular behavior. The whole job serves as a paradigm for sensor creating.

Light
Communication

The Luminesensor is able to respond to very low light and meanwhile keep a wide dynamic range, which encouraged us to explore the possibility of cell-cell communication through light. We have successfully implemented, for the very first time, light-communication among cells without direct physical contact.

Syn Bio in
2D & 3D

3D printing is a new technology that has been rising for many years. But in the realm of synthetic biology, it is far from developed. We exploited our Luminesensor to implement 3D printing that can be utilized in many medical or manufacturing applications.

Phototaxis

"Phototatic" bacteria can be built by programming the chemotaxis system in E. coli through light. By controlling the expression level of the CheZ protein with the Luminesensor, the tumbling frequency is coupled to the intensity of light signals.

Human
Practice

We have done a remarkable job in motivating high school students to study synthetic biology and guiding them towards future participation in the iGEM high school division. Besides, we collaborated with a lab and helped four other iGEM teams by sharing DNA materials, characterizing their parts and modeling. Furthermore, we presented all fresh iGEMers with a collection of praise for historic iGEM projects.

Modeling

Excellent stochastic simulation was conducted to describe our ultrasensitive Luminesensor, combining protein kinetics and reaction thermodynamics. Molecular docking was also operated. What's more, while designing our photo-taxis model, we developed a hexagonal-coordinate environment for dynamic simulation of "on-plate" system. Those who focus on cell motility will find it useful and inspirational.

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 Members
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 Overview
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-BioBrick Data
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-Luminesensor Optimization
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-   <div style="float:left;"><p style="font-size:45px;line-height:45px;color:#ffffff;margin:20px 3px 0 20px;">O</p></div>
+   <div style="float:left;"><p style="font-size:45px;line-height:45px;color:#ffffff;margin:20px 3px 0 20px;">L</p></div>
    <p class="context_block" style="width:610px;">
-ptogenetic tools have made significant impact on life sciences and beyond. However, several serious issues remain: cytotoxicity, narrow dynamic range, and dependency on laser and exogenous chromophores. To unblock the bottleneck, Peking iGEM has rationally constructed a hypersensitive sensor of luminance -- <b><i>Luminesensor</i></b>. Primarily, the sensor was designed by fusing the blue-light-sensing protein domain from <i>Neurospora</i> with the DNA binding domain of LexA from <i>E. coli</i>. Subsequently, protein structure inspection and kinetic simulation were conducted to rationally perform optimization. Amazingly, <i>Luminesensor</i> was proved to be so sensitive as to sense <b>natural light</b> and even <b>bioluminescence</b>. With this sensor, spatiotemporal control of cellular behavior, such as <b>high-resolution 2D</b> and <b>3D bio-printing</b> using dim light and even <b>luminescence of iPad</b> were shown to be very easy. What’s more, we successfully implemented <b>cell-cell signaling using light</b>, which is the very first time in synthetic biology and of great importance for biotechnological use.
+ight has created innumerable wonders in nature. Equipped with tools and methods in optogenetic research, scientists have also achieved some truly fascinating goals in a synthetic way. This year, 2012 Peking-ECUST iGEM utilized an ultra-sensitive engineered sensor of luminescence -- the <b style="color:#ffee55;"> <i>Luminesensor</i></b>. Amazingly, the <i>Luminesensor</i> was proven to be so sensitive as to be able to detect <b style="color:#ffee55;">natural light</b> and even <b style="color:#ffee55;">bioluminescence</b>. With this sensor, spatiotemporal control of cellular behavior, such as <b style="color:#ffee55;">high-resolution 2D</b> and <b style="color:#ffee55;">3D bio-printing</b> using dim light and even the <b style="color:#ffee55;">luminescence of iPad</b> were shown to be very easy. What’s more, we successfully implemented <b style="color:#ffee55;">cell-cell signaling using light</b> for the very first time in synthetic biology, which will be of great importance for biotechnological use.
    </p>
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-  <div class="floatL" style="margin:0 10px 5px 0;"><table class="title_block" style="background-image:url('/wiki/images/b/be/Peking2012_Color_Title_AquaBlue.jpg');"><tr><td style="height:165px;"><p style="font-size:36px;">Abstract</p></td></tr></table></div>
+  <div class="floatL" style="margin:0 10px 5px 0;"><table class="title_block" style="background-image:url('/wiki/images/b/be/Peking2012_Color_Title_AquaBlue.jpg');"><tr><td style="height:244px;"><p style="font-size:36px;">Abstract</p></td></tr></table></div>
- <div class="floatL" style="margin:10px 0 5px;"><p style="font-size:19px;font-family:Verdana;color:#ffd320;width:200px;text-align:center;"><b>Peking iGEM would never disappoint you!</b></div>
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    <div class="floatR"><img src="/wiki/images/4/4f/Peking2012_LuminesensorMechanism.jpg" alt="" style="width:200px;"/></div>
    <div class="floatC"><p class="context_block" style="width:360px;">
-Strongly motivated to open up a new era of optogenetics, Peking iGEM team has rationally constructed a hypersensitive sensor of luminance – named by us <i>Luminesensor</i>, enabling highly efficient spatiotemporal control of biochemical process or cellular behavior. The whole job serves as a paradigm for sensor creating.
+Strongly motivated to open up a new era of optogenetics, our team has utilized an ultra-sensitive sensor which is engineered for sensing luminance – named by us <i>Luminesensor</i>, enabling highly efficient spatiotemporal control of biochemical process or cellular behavior. The whole job serves as a paradigm for sensor creating.
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    <div class="floatC"><p class="context_block" style="width:360px;">
-The hypersensitive <i>Luminesensor</i> is able to respond to very low light and meanwhile keep a wide dynamic range, which encouraged Peking iGEM to explore the possibility of cell-cell communication through light. We have successfully implemented, for the very first time, light-communication among cells without direct physical contact.
+The <i>Luminesensor</i> is able to respond to very low light and meanwhile keep a wide dynamic range, which encouraged us to explore the possibility of cell-cell communication through light. We have successfully implemented, for the very first time, light-communication among cells without direct physical contact.
    </p></div>
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    <div class="floatR"><img src="/wiki/images/b/b1/Peking2012_PrintParagraph_Square_Edit.jpg" alt="" style="width:200px;"/></div>
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-D printing is a new technology that has been rising for many years. But in the realm of synthetic biology, it is far from developed. We exploited our <i>Luminesensor</i> to implement 3D printing that can be utilized in many applications in medical or manufacturing.
+D printing is a new technology that has been rising for many years. But in the realm of synthetic biology, it is far from developed. We exploited our <i>Luminesensor</i> to implement 3D printing that can be utilized in many medical or manufacturing applications.
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-"Phototatic" bacteria can be built by programming the chemotaxis system in <i>E. coli</i> through light. By controlling the expression level of the CheZ protein with <i>Luminesensor</i>, the tumbling frequency is coupled to the intensity of light signals.
+"Phototatic" bacteria can be built by programming the chemotaxis system in <i>E. coli</i> through light. By controlling the expression level of the CheZ protein with the <i>Luminesensor</i>, the tumbling frequency is coupled to the intensity of light signals.
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-We have done a remarkable job in motivating high school students to study synthetic biology and guiding them towards future participation in the iGEM high school division. Besides, we collaborated with a lab and helped 4 other iGEM teams by sharing DNA materials, characterizing their parts and modeling. What's more, we presented all fresh iGEMers with a collection and praise of historic iGEM projects to share and learn from each other!
+We have done a remarkable job in motivating high school students to study synthetic biology and guiding them towards future participation in the iGEM high school division. Besides, we collaborated with a lab and helped four other iGEM teams by sharing DNA materials, characterizing their parts and modeling. Furthermore, we presented all fresh iGEMers with a collection of praise for historic iGEM projects.
    </p></div>
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    <div class="floatC"><p class="context_block" style="width:360px;">
-We conducted excellent modeling to rationally design our <i>Luminesensor</i>, combining protein kinetics, thermodynamics, and stochastic simulation with molecular docking together. On the other hand, when modeling the process of photo-taxis, we also developed a hexagonal-coordinate simulation environment for dynamic system on a continuous plane, which would be a very useful prototype for future simulation on cellular motion.
+Excellent stochastic simulation was conducted to describe our ultrasensitive <i>Luminesensor</i>, combining protein kinetics and reaction thermodynamics. Molecular docking was also operated. What's more, while designing our photo-taxis model, we developed a hexagonal-coordinate environment for dynamic simulation of "on-plate" system. Those who focus on cell motility will find it useful and inspirational.
    </p></div>
   </div>