Team:Korea U Seoul/Notebook/Aug
From 2012.igem.org
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<h4><p id="title">Notebook : August</p></h4> | <h4><p id="title">Notebook : August</p></h4> | ||
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<b> A. August, 1, 2012 </b> | <b> A. August, 1, 2012 </b> | ||
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- | <p style="padding-left:30px;">We have discussed about how to dectect and kill ' | + | <p style="padding-left:30px;">We have discussed about how to dectect and kill 'Xanthomonas oryzae’</p> |
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- | is it possible for | + | is it possible for Xanthomonas to produce AX21 without plant receptor? |
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- | Research | + | Research Xanthomonas : Jeongmin |
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<b> B. August 8, 2012 </b> | <b> B. August 8, 2012 </b> | ||
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- | <p style="padding-left:30px;">We have discussed about how to dectect and kill ' | + | <p style="padding-left:30px;">We have discussed about how to dectect and kill 'Xanthomonas oryzae.’ Solidifying the idea and circuit</p> |
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- | <p style="padding-left:30px;">We have discussed about how to dectect and kill ' | + | <p style="padding-left:30px;">We have discussed about how to dectect and kill 'Xanthomonas oryzae.’ Solidifying the idea and circuit</p> |
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<b> Idea development </b> | <b> Idea development </b> | ||
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- | Jeongmin Lee: | + | Jeongmin Lee: Xanthomonas Abstract & background |
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- | Hyunkee Kim : | + | Hyunkee Kim : Xanthomonas Abstract & background, Note-book, Safety question |
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Wonwook Lee : get sponsor | Wonwook Lee : get sponsor | ||
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+ | <br><br><br> | ||
+ | <dt> | ||
+ | <b> D. August 28, 2012 </b> | ||
+ | </dt> | ||
+ | <dd> | ||
+ | <b> 1) checked our progress on: </b> | ||
+ | <br> | ||
+ | <p> | ||
+ | modeling | ||
+ | </p> | ||
+ | <p> | ||
+ | notebook, safety, design | ||
+ | </p> | ||
+ | <p> | ||
+ | wiki main page flash file, team logo | ||
+ | </p> | ||
+ | <p> | ||
+ | sponsor | ||
+ | </p> | ||
+ | <p> | ||
+ | facebook self intro and photo | ||
+ | </p> | ||
+ | |||
<br> | <br> | ||
- | <b> | + | <b> 2) What we have to do before next meeting: </b> |
+ | <p> | ||
+ | notebook translation and safety question upload | ||
+ | </p> | ||
+ | <p> | ||
+ | Finishing up main page flash file for wiki page | ||
+ | </p> | ||
+ | <p> | ||
+ | Logo for our team | ||
+ | </p> | ||
+ | <p> | ||
+ | fundraising | ||
+ | </p> | ||
+ | <p> | ||
+ | use every possible resources to get sponsor! | ||
+ | </p> | ||
+ | <p> | ||
+ | book flight tickets for Hong Kong | ||
+ | </p> | ||
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+ | <br> | ||
+ | <div id="wrapperB" style="width:603px; height:63px; "> | ||
+ | <a href="http://korea.ac.kr"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/a/ab/KUS_Ku.jpg" style="padding-top:10px;" width="80px" ></a> | ||
+ | |||
+ | <a href="http://compbio.korea.ac.kr/wiki/index.php/Main_Page"> <img src="https://static.igem.org/mediawiki/2012/5/5d/KUS_Csbl.jpg" width="82px" ></a> | ||
+ | |||
+ | <a href="http://ctl.korea.ac.kr/index.ctl"> <img src="https://static.igem.org/mediawiki/2012/4/49/KUS_Ctl.jpg" width="75px" ></a> | ||
+ | |||
+ | <a href="http://www.cosmogenetech.com/ko"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/8/81/KUS_Cosmo.jpg" width="75px" height="70"></a> | ||
+ | |||
+ | |||
+ | <a href="http://www.youtube.com/watch?v=vr7TlPjyAEg"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/c/c4/KUS_Youtube.jpg" width="65px" ></a> | ||
+ | |||
+ | |||
+ | <a href="http://www.facebook.com/2012iGEM.KU"> <img src="https://static.igem.org/mediawiki/2012/7/71/KUS_Facebook.jpg" width="52px"></a> | ||
+ | |||
+ | |||
+ | <a href="https://twitter.com/iGEM_KU2012"> <img src="https://static.igem.org/mediawiki/2012/4/4d/KUS_Twitter.jpg" width="63px"></a> | ||
+ | </div> | ||
+ | </div> | ||
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</div> | </div> |
Latest revision as of 02:15, 27 September 2012
Notebook : August
We have discussed about how to dectect and kill 'Xanthomonas oryzae’
Haerim Song
raxR/H is target for raxSTAB.
Sulfur plays an important role in activity of AX21; it won’t work without sulfur
overexpression of raxST(sulfur attachment to AX21), RaxR/Q(transformation of PAPS into ATP) is possible in E.coli
Advisor’s advicetest any promoter which binds to raxR/H
is it possible for Xanthomonas to produce AX21 without plant receptor?
(Professor’s comment) we can run a test using E/coli which has plant receptor
Method for detecting AX21 production?
(Professor’s comment) use mass spectrometry or put florescence after ax21 gene then cut with restriction enzyme
Jihoon JungFind A
Kyeongwoo Jang’s opinionfind system which utilizes logic gate in natural world
2) Next meeting
(1) research rax operon
How to detect Ax21 : HyunKee Kim
Research raxR/H paper : Byeongnam Min
Research Xanthomonas : Jeongmin
Activity of the substance only with raxR/H: Haerim Song
(2) team
Logic gate: Jihoon Jung, Kyeongwoo Jang
Science camp presentation: Haerim Song
Wiki update: Byeongnam Min, Kyeongwoo Jang, Sanghoon Han
Notebook translation: HyunKee Kim
Study Modeling: Sanghoon Han, Byeongnam Min
Design team: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam, Haerim Song
We have discussed about how to dectect and kill 'Xanthomonas oryzae.’ Solidifying the idea and circuit
raxR, raxH can be detected using His tag; however, experiments using cloning technique don’t use this.
No indication of functional opetation only with raxR, raxH
Haerim SongraxH without transmembrane segment was autophosphorylation, but no raxR transphosphrylation.
raxH H222 and RaxR D51 is responsible for phosphorylation.
Lab teamacquisition of PCEL, raxRH promoter, mRFP using PCR
cloning is expected to be held this week
raxR/H is constitutive, so use other promoter
2) Team placement and H.W
logic gate: find A: Sanghoon Han, Jihoon Jung, Kyeongwoo Jang
Ax21 production: Wonwook Lee, JungMin, Kyeongjin, Haerim Song, Byeongnam Min, Hyunkee Kim
Brochure: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam, Haerim Song
Science camp presentation: Haerim Song
Wiki update: Nam, Byeongnam Min, Kyeongwoo Jang, Sanghoon Han
Notebook translation: HyunKee Kim
Study Modeling: Sanghoon Han, Byeongnam Min
Design team: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam, Haerim Song
CCP: Sanghoon Han
KAIST Contact; Byeongnam Min
Team attire: Jihoon Jung
upload at least one copy of design to the homepage (mandatory)
We have discussed about how to dectect and kill 'Xanthomonas oryzae.’ Solidifying the idea and circuit
Kyeongwoo Jang: logic gate biosensing
researched logic gate used by organism to detect certain chemical
British Columbia University already did similar project; it was about concentration dependent color changing bacteria.
Logic gate using single step is more efficient than using several input and output steps
Sanghoon Han: finding ‘a’ chemicalDSF(diffusible signaling factor)
Haerim Song: bacterial leaf blighthad presentation about iGEM during ‘Lifescience camp’
in order to prevent bacterial leaf blight, chemical-pesticide was given. However, it was proven to be ineffective.
Bacterial leaf blight resistant plant was developed but, later sub-population eliminated effect of ‘gene R.’
Wonwook Lee: sponsorLG lifescience, Celltrion, Rural development administration, cosmogenetech: yet positive reply, he will continue to contact.
Sanghoon HanSmall Protein-Mediated Quorum Sensing in a Gram- Negative Bacterium (PMID : 22174954) To elucidate this function, we previously isolated and characterized eight genes required for Ax21 activity (rax genes). raxA, raxB and raxC encode components of a predicted type I secretion system (TOSS). Ax21requires this RaxABC TOSS for activity and secretion [3,4]. The RaxB protein carries two highly conserved N terminal proteolytic subdomains characteristic of transporters in Gram-positive bacteria that cleave N-terminal peptides prior to substrate secretion [4]. These data, together with the presence of a predicted N-terminal signal sequence in Ax21, suggest that Ax21 is cleaved by the RaxB transporter prior to secretion. raxST, raxP and raxQ encode enzymes involved in sulfation
This indicates AX21 is synthesized without a plant
2) Design
Benchmark : http://www.linocoms.co.kr/index.htm
need to use ‘Flash’ program
3) CCP: report due on 24th (logic gate and DIY)
We discussed about our wiki page design, content, uniform design, and part allocation.
Regional Jamboree registration : 275 USD
Airfare : 40~50 KRW
Hotel reservation : iGEM will propose several hotels in a discount charge
2) assigned part
Sanghoon Han : CCP report, video making, researching logic gate background & design, modeling, Note-book, protocol production
Jihoon Jung : CCP report, video making, uniform order(later)
Kyeongwoo Jang: CCP report , video making, logic gate background & design
Jeongmin Lee: Xanthomonas Abstract & background
Hyunkee Kim : Xanthomonas Abstract & background, Note-book, Safety question
Haerim Song: mainframe design, poster design(later)
Byeongnam Min: modeling
Jihyong Nam: protocol
Kyeongjin Kim: get sponsor
Wonwook Lee : get sponsor
modeling
notebook, safety, design
wiki main page flash file, team logo
sponsor
facebook self intro and photo
2) What we have to do before next meeting:
notebook translation and safety question upload
Finishing up main page flash file for wiki page
Logo for our team
fundraising
use every possible resources to get sponsor!
book flight tickets for Hong Kong