Team:Lyon-INSA/datapage

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<h1>Interactive pattern of our construction : Toggle switch option</h1>
<h1>Interactive pattern of our construction : Toggle switch option</h1>
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<center><strong>Hover your mouse over a step number/letter to see more</strong></center><br/>
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<h1>Our parts : </h1>
<h1>Our parts : </h1>
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<big><font color ="#084A42"><u><b>Data about our favorite parts :</b></u></font></big><br>
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<div style="margin:20px;font-size:15px;">
 +
<div class="petitSsTitre">Data about our favorite new parts </div>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802001" target="_blank"><font color="#664499"><b>1. Main Page</b></font></a>: <b>Dispersin generator for <i>B. subtilis</i></b> BBa_K802001 : This part associates the <i>Bacillus subtilis</i> constitutive promoter (P<sub>veg</sub>) with the dispersin B gene (<i>dspB</i>). This part allows to efficiently scatter Staphylococci biofilms. <br>
 +
 
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<br><br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802000" target="_blank"><font color="#664499"><b>2. Main Page</b></font></a>: <b>Lysostaphin generator for <i>B. subtilis</i></b> BBa_K802000  : This part associates the <i>Bacillus subtilis</i> constitutive promoter (P<sub>veg</sub>) with the lysostaphin gene. This part allows an efficient killing of <i>S. aureus</i> cells.<br><br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802009" target="_blank"><font color="#664499"><b>3. Main Page</b></font></a>: <b>Surfactin generator and biofilm repressor for <i>B. subtilis</i></b> BBa_K802009: This part can be used to induce surfactin production and to repress the biofilm formation in <i> B. subtilis</i> strains (COAT module).<font color="RED"><b>NEW</b></font>  
<br>
<br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802000" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>Lysostaphin generator for <i>B. subtilis</i></b> BBa_K802000 : This part associates the <i>Bacillus subtilis</i> Constitutive Promoter (P<sub>veg</sub>) with the lysostaphin gene. In our plasmid collection, this part is named pBK23 in the pSB1C3 backbone (Cm<sup>R</sup>) and pBKL28 in the shuttle vector <i>E. coli</i> – <i>B. subtilis</i>. The pBKL28 plasmid was transformed in the NM522 strain to perform tests in <i>E. coli</i> and in the 168 strain to perform tests in <i>Bacillus subtilis</i>.<br>
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<div class="petitSsTitre">We've also characterized the following new parts </div>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802002" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>P<sub>lac</sub>-RBS-GFP</b>  BBa_K802002 : This part has been designed to determine the behavior of the P<sub>lac</sub> promoter used to drive the STICK module (<span class="unProto" onclick="window.open('http://partsregistry.org/Part:BBa_K802009', 'Part BBa_K802009'); return false;">Part BBa_K802009</span>)
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<br>
<br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802001" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>Dispersin generator for <i>B. subtilis</i></b> BBa_K802001 : This part associates the <i>Bacillus subtilis</i> Constitutive Promoter (P<sub>veg</sub>) with the dispersin B gene (<i>dspB</i>). In our plasmid collection, this part is named pBK33 in the Chloramphenicol backbone and pBKH41 in the shuttle vector <i>E. coli</i> – <i>B. subtilis</i>. We worked with the plasmid pBKH41 for the tests and we tried two different genetic backgrounds : the NM522 strain to make test in <i>E. coli</i> and the 168 strain to make test in <i>Bacillus subtilis</i>.<br>
 
<br>
<br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802002" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>P<sub>lac</sub>(<i>B. subtilis</i>)-RBS(<i>E. coli</i>)-GFP</b> BBa_K802002 : This part is used to determine if the promoter P<sub>lac</sub> works or not and especially how it works when it is activated by different concentration of the inductor IPTG. Within this part there is P<sub>lac</sub> from <i>Bacillus subtilis</i> which is the promoter that induces production of XylR to form a positive biofilm. Then a RBS from <i>E coli</i> is added to finally enable production of GFP which is an easy measurable parameter using the amount of fluorescence.<br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802003" target="_blank"><font color="#664499"><b> Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b>BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>low</b> copy number plasmid in <i>B. subtilis</i> and a high copy number plasmid in <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in <i>B. subtilis</i> by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.
<br>
<br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802003" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b>BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a low copy number plasmid in <i>B. subtilis</i> and a high copy number plasmid in <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. <br>
 
<br>
<br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802004" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b> BBa_K802004 : This is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a high copy number plasmid in both <i>B. subtilis</i> and <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites.<br>
 
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<br>
 
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802005" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004</b> BBa_K802005 : This part is a custom designed polylinker which was cloned into the shuttle vectors BBa_K802004 and BBa_K802003 so that the plasmids would correspond to the iGEM format.<br>
 
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<br/><br/>
 
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802004" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b> BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>high</b> copy number plasmid in both <i>B. subtilis</i> and <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in <i>B. subtilis</i> by a transformation yield of 80 transformants/µg and a erythromycin resistance to at least 1.5 mg/mL.
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<br><br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802007" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Biofilm repressor for <i>B. subtilis</i> strains</b> BBa_K802007 : This part can be used to impede biofilm formation in <i>B. subtilis</i>, more particularly if it is arbB-. This part was characterized in conjuction with parts K802006 and K802008.<font color="RED"><b>NEW</b></font>
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{{Lyon-INSA/pub}}
{{Lyon-INSA/pub}}

Latest revision as of 00:40, 27 October 2012

Interactive pattern of our construction : Toggle switch option

Hover your mouse over a step number/letter to see more


Our parts :

Data about our favorite new parts
1. Main Page: Dispersin generator for B. subtilis BBa_K802001 : This part associates the Bacillus subtilis constitutive promoter (Pveg) with the dispersin B gene (dspB). This part allows to efficiently scatter Staphylococci biofilms.


2. Main Page: Lysostaphin generator for B. subtilis BBa_K802000 : This part associates the Bacillus subtilis constitutive promoter (Pveg) with the lysostaphin gene. This part allows an efficient killing of S. aureus cells.

3. Main Page: Surfactin generator and biofilm repressor for B. subtilis BBa_K802009: This part can be used to induce surfactin production and to repress the biofilm formation in B. subtilis strains (COAT module).NEW
We've also characterized the following new parts
Main Page: Plac-RBS-GFP BBa_K802002 : This part has been designed to determine the behavior of the Plac promoter used to drive the STICK module (Part BBa_K802009)

Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low copy number plasmid in B. subtilis and a high copy number plasmid in E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.

Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high copy number plasmid in both B. subtilis and E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 80 transformants/µg and a erythromycin resistance to at least 1.5 mg/mL.

Main Page: Biofilm repressor for B. subtilis strains BBa_K802007 : This part can be used to impede biofilm formation in B. subtilis, more particularly if it is arbB-. This part was characterized in conjuction with parts K802006 and K802008.NEW


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