Team:Paris-Saclay/Project/Notebook/Week 10

From 2012.igem.org

(Difference between revisions)
(→7th August)
 
(14 intermediate revisions not shown)
Line 1: Line 1:
 +
<div id="large-single-paris-saclay">
 +
{{Team:Paris-Saclay/Header}}
 +
<div id="single-paris-saclay">
 +
{{Team:Paris-Saclay/Menu}}
 +
<div id="single-left-column">
 +
<html>
 +
<head>
 +
        <link rel="stylesheet" type="text/css" href="https://2012.igem.org/Team:Paris-Saclay/th-accordion.css?action=raw&ctype=text/css" />
 +
        <link rel="stylesheet" type="text/css" href="https://2012.igem.org/Team:Paris-Saclay/st-accordion.css?action=raw&ctype=text/css" />
 +
        <link href='http://fonts.googleapis.com/css?family=Josefin+Slab:400,700' rel='stylesheet' type='text/css' />
 +
<script type="text/javascript" src='/Team:Paris-Saclay/jquery.min.js?action=raw&ctype=text/javascript'></script>
 +
<script type="text/javascript" src='/Team:Paris-Saclay/MetroJs.js?action=raw&ctype=text/javascript'></script>
 +
<noscript>
 +
<style>
 +
.st-accordion ul li{
 +
height:auto;
 +
}
 +
.st-accordion ul li > a span{
 +
visibility:hidden;
 +
}
 +
</style>
 +
</noscript>
 +
</head>
 +
<body>
 +
<div id="tiles">
 +
<div id="single-tile3" class="red live-tile" data-mode="flip" data-delay="4000">
 +
        <div>
 +
<a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract">
 +
<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div>
 +
            <img src="https://static.igem.org/mediawiki/2012/7/7c/Project-1.png" alt="" />
 +
</a>
 +
        </div>
 +
<div>
 +
<a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract">
 +
<img src="https://static.igem.org/mediawiki/2012/d/d1/Project2.jpg" alt="" />
 +
<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div>
 +
</a>
 +
</div>
 +
</div>
 +
<script type="text/javascript">
 +
    // apply regular slide universally unless .exclude class is applied
 +
    // NOTE: The default options for each liveTile are being pulled from the 'data-' attributes
 +
    $(".live-tile, .flip-list").not(".exclude").liveTile();
 +
</script>
 +
</div>
 +
</body>
 +
</html>
 +
{{Team:Paris-Saclay/left-column}}
 +
</div>
 +
<div id="content-paris-saclay">
 +
='''Week 10'''=
 +
 +
[[Category:Team:Paris-Saclay/Project Gemote/Notebook|j]]
__NOTOC__
__NOTOC__
====6th August====
====6th August====
Line 10: Line 63:
*Liquid culture of B1 in order to prepare a glycerol stock
*Liquid culture of B1 in order to prepare a glycerol stock
-
**Receipt of new primers
+
**Reception of new primers
**2 Forward
**2 Forward
**3 Reverse
**3 Reverse
**Plasmid Reverse
**Plasmid Reverse
-
 
====8th August====
====8th August====
Line 44: Line 96:
|-
|-
| style="width: 50%;"|Amplification by PCR of BBa_K274100 already digested by EcoRI. Visualization by electrophoresis on a 0.8% Agarose gel.
| style="width: 50%;"|Amplification by PCR of BBa_K274100 already digested by EcoRI. Visualization by electrophoresis on a 0.8% Agarose gel.
-
| style="width: 35%;"| [[File:Week10-5.jpg|right|330px]]
+
| style="width: 35%;"| [[File:Week10-5.jpg|right|250px]]
|}
|}
PCR program used:
PCR program used:
-
[[File:Week10-6.jpg|600px]]
+
[[File:Week10-8.jpg|400px]]
{| width="85%"
{| width="85%"
|-
|-
| style="width: 50%;"|Miniprep of BBa_K274100 followed by Nanovue to determine the concentration of the sample
| style="width: 50%;"|Miniprep of BBa_K274100 followed by Nanovue to determine the concentration of the sample
-
| style="width: 35%;"| [[File:Week10-5.jpg|right|330px]]
+
| style="width: 35%;"| [[File:Week10-6.jpg|right|330px]]
|-
|-
| style="width: 50%;"|Miniprep of the Plasmid pSB1A2 followed by Nanovue to determine the concentration of the sample
| style="width: 50%;"|Miniprep of the Plasmid pSB1A2 followed by Nanovue to determine the concentration of the sample
-
| style="width: 35%;"| [[File:Week10-6.jpg|right|330px]]
+
| style="width: 35%;"| [[File:Week10-7.jpg|right|330px]]
|}
|}
Line 65: Line 117:
|-
|-
| style="width: 50%;"|Digestion of BBa_K115017 by DPNI to eliminate the plasmid matrix. Visualization by electrophoresis on a 2% Agarose gel. We are expecting a band at 123 bp.
| style="width: 50%;"|Digestion of BBa_K115017 by DPNI to eliminate the plasmid matrix. Visualization by electrophoresis on a 2% Agarose gel. We are expecting a band at 123 bp.
-
| style="width: 35%;"| [[File:Week10-7.jpg|right|330px]]
+
| style="width: 35%;"| [[File:Week10-1.jpg|right|280px]]
|}
|}
 +
 +
{{Team:Paris-Saclay/Follow}}
 +
</div>
 +
</div>
 +
</div>
 +
{{Team:Paris-Saclay/Footer}}

Latest revision as of 00:32, 27 September 2012

Follow us !

Week 10

6th August

  • Sending of the B1 sample to sequencing with two pairs of primers
    • K-274100 Forward and Reverse
    • Plasmid pSB1A2 Forward and Reverse


7th August

  • Liquid culture of B1 in order to prepare a glycerol stock
    • Reception of new primers
    • 2 Forward
    • 3 Reverse
    • Plasmid Reverse

8th August

Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.
Week10-2.jpg


PCR program used: Week10-3.jpg

9th August

New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before.
Week10-4.jpg
  • Digestion by EcoRI of the plasmid that contains BBa_K274100.


10th August

Amplification by PCR of BBa_K274100 already digested by EcoRI. Visualization by electrophoresis on a 0.8% Agarose gel.
Week10-5.jpg


PCR program used: Week10-8.jpg

Miniprep of BBa_K274100 followed by Nanovue to determine the concentration of the sample
Week10-6.jpg
Miniprep of the Plasmid pSB1A2 followed by Nanovue to determine the concentration of the sample
Week10-7.jpg
  • Digestion by HindIII of the plasmid pSB1A2 in order to linearize it.
Digestion of BBa_K115017 by DPNI to eliminate the plasmid matrix. Visualization by electrophoresis on a 2% Agarose gel. We are expecting a band at 123 bp.
Week10-1.jpg

Retrieved from "http://2012.igem.org/Team:Paris-Saclay/Project/Notebook/Week_10"