Team:Lyon-INSA/datapage

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<h1>Interactive pattern of our construction : Toggle switch option</h1>
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<center><strong><big>Hover your mouse over a step number/letter to see more</big></strong></center><br/>
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<h1>Our parts : </h1>
<h1>Our parts : </h1>
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<big><font color ="#084A42"><u><b>Data for our favorite new parts :</b></u></font></big><br>
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<div style="margin:20px;font-size:15px;">
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<div class="petitSsTitre">Data about our favorite new parts </div>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802001" target="_blank"><font color="#664499"><b>1. Main Page</b></font></a>: <b>Dispersin generator for <i>B. subtilis</i></b> BBa_K802001 : This part associates the <i>Bacillus subtilis</i> constitutive promoter (P<sub>veg</sub>) with the dispersin B gene (<i>dspB</i>). This part allows to efficiently scatter Staphylococci biofilms. <br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802000" target="_blank"><font color="#664499"><b>2. Main Page</b></font></a>: <b>Lysostaphin generator for <i>B. subtilis</i></b> BBa_K802000  : This part associates the <i>Bacillus subtilis</i> constitutive promoter (P<sub>veg</sub>) with the lysostaphin gene. This part allows an efficient killing of <i>S. aureus</i> cells.<br><br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802009" target="_blank"><font color="#664499"><b>3. Main Page</b></font></a>: <b>Surfactin generator and biofilm repressor for <i>B. subtilis</i></b> BBa_K802009: This part can be used to induce surfactin production and to repress the biofilm formation in <i> B. subtilis</i> strains (COAT module).<font color="RED"><b>NEW</b></font>  
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802000" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>Lysostaphin generator for <i>B. subtilis</i></b> BBa_K802000 : This part associates the Bacillus subtilis Constitutive Promoter (PVeg) with the lysostaphin gene. In our plasmid collection, this part is named pBK23 in the backbone pSB1C3 (cmR) and pBKL28 in the shuttle vector <i>E. coli</i> – <i>B. subtilis</i>. The pBKL28 plasmid was introduced in the strain NM522 to perform tests in <i>E. coli</i> and in the strain 168 to perform tests in <i>Bacillus subtilis</i>.<br>
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<div class="petitSsTitre">We've also characterized the following new parts </div>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802002" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>P<sub>lac</sub>-RBS-GFP</b>  BBa_K802002 : This part has been designed to determine the behavior of the P<sub>lac</sub> promoter used to drive the STICK module (<span class="unProto" onclick="window.open('http://partsregistry.org/Part:BBa_K802009', 'Part BBa_K802009'); return false;">Part BBa_K802009</span>)
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802001" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>Dispersin generator for <i>B. subtilis</i></b> Ba_K802001 : This part associates the <i>Bacillus subtilis</i> Constitutive Promoter (PVeg) with the dispersin B gene (DspB). In our plasmid collection, this part is named pBK33 in the backbone Chloramphenicol and pBKH41 in the shuttle vector <i>E. coli</i> – <i>B. subtilis</i>. We worked with the plasmid pBKH41 for the tests and we tried two different genetic backgrounds: the strain NM522 to make test in <i>E. coli</i> and the strain 168 to make test in <i>Bacillus subtilis</i>.<br>
 
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802002" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>Plac(B. subtilis)-RBS(<i>E. coli</i>)-GFP</b> Ba_K802002 :This part is used to determine if the promoter pLac works or not and especially how it works when it is activated by different concentration of the inductor IPTG. Within this part there is pLac from Bacillus subtilis which is the promoter that induces a xylR production to form a positive biofilm. Then a RBS from <i>E coli</i> is added to allow it and finally the GFP which is an easy measurable parameter using the amount of fluorescence.<br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802003" target="_blank"><font color="#664499"><b> Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b>BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>low</b> copy number plasmid in <i>B. subtilis</i> and a high copy number plasmid in <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in <i>B. subtilis</i> by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802003" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b>Ba_K802003 :<br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802004" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b> BBa_K802004 :<br>
 
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802005" target="_blank"><font color="purple"><b>Main Page</b></font></a>: <b>iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004</b> BBa_K802005 :<br>
 
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<center><strong>Put your mouse over a step number/letter to see more</strong></center>
 
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802004" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b> BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>high</b> copy number plasmid in both <i>B. subtilis</i> and <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in <i>B. subtilis</i> by a transformation yield of 80 transformants/µg and a erythromycin resistance to at least 1.5 mg/mL.
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802007" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Biofilm repressor for <i>B. subtilis</i> strains</b> BBa_K802007 : This part can be used to impede biofilm formation in <i>B. subtilis</i>, more particularly if it is arbB-. This part was characterized in conjuction with parts K802006 and K802008.<font color="RED"><b>NEW</b></font>  
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{{Lyon-INSA/pub}}
{{Lyon-INSA/pub}}

Latest revision as of 00:40, 27 October 2012

Interactive pattern of our construction : Toggle switch option

Hover your mouse over a step number/letter to see more


Our parts :

Data about our favorite new parts
1. Main Page: Dispersin generator for B. subtilis BBa_K802001 : This part associates the Bacillus subtilis constitutive promoter (Pveg) with the dispersin B gene (dspB). This part allows to efficiently scatter Staphylococci biofilms.


2. Main Page: Lysostaphin generator for B. subtilis BBa_K802000 : This part associates the Bacillus subtilis constitutive promoter (Pveg) with the lysostaphin gene. This part allows an efficient killing of S. aureus cells.

3. Main Page: Surfactin generator and biofilm repressor for B. subtilis BBa_K802009: This part can be used to induce surfactin production and to repress the biofilm formation in B. subtilis strains (COAT module).NEW
We've also characterized the following new parts
Main Page: Plac-RBS-GFP BBa_K802002 : This part has been designed to determine the behavior of the Plac promoter used to drive the STICK module (Part BBa_K802009)

Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low copy number plasmid in B. subtilis and a high copy number plasmid in E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.

Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high copy number plasmid in both B. subtilis and E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 80 transformants/µg and a erythromycin resistance to at least 1.5 mg/mL.

Main Page: Biofilm repressor for B. subtilis strains BBa_K802007 : This part can be used to impede biofilm formation in B. subtilis, more particularly if it is arbB-. This part was characterized in conjuction with parts K802006 and K802008.NEW


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