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| - | __NOTOC__
| + | {{Template:Caltech_Header}} |
| - | <html>
| + | |
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| | | | |
| - | <style> | + | <html> |
| - | h1.firstHeading { display: none; }
| + | |
| | | | |
| - | p {text-align: justify;}
| + | <h1><a name="Materials_and_Methods";>Materials and Methods</a></h1> |
| | + | <p>Here is a list of all the procedures we used this summer. Click on a protocol to read its description and the materials required.</p> |
| | | | |
| - | a:link { color: #00b0e6; text-decoration: none}
| + | <ul> |
| - | a:visited { color:#00b0e6; text-decoration: none} | + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#electrocompetent_cells";>Electrocompetent Cells</a></li> |
| - | a:hover { color:#f29400; text-decoration: none} | + | |
| - | a:active { color:#f29400; text-decoration: none}
| + | |
| | | | |
| - | #bodyContent { padding: 10px auto; width: 910px; margin: auto; clear: none; }
| + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#Transformation_by_Electroporation";>Transformation by Electroporation</a></li> |
| | | | |
| - | table#team_members { text-align: justify; border: 0; }
| + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#Transformation_from_distribution";>Transformation from Distribution</a></li> |
| - | table#team_members h2, table#team_members h3 { clear: both; }
| + | |
| | | | |
| | + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#Cell_Transformation";>Cell Transformation</a></li> |
| | | | |
| - | /*-----------------------------------------------------------------------------------------------*/ | + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#Gene_Assembly";>Gene Assembly</a></li> |
| - | div.MenuBar ul li ul.DropDownMenu {
| + | |
| - | display: none; /* Hides all drop-down menus. */
| + | |
| | | | |
| - | }
| + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#Overlay_plates";>Overlay Plates</a></li> |
| - | /*
| + | |
| - | li:hover works in IE7 and FF2. | + | |
| - | a:hover works in IE6 and FF2. | + | |
| - | a:hover breaks li:hover in FF2.
| + | |
| - | */
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
| + | |
| - | display: none; /* Hides all side menus. */
| + | |
| - | }
| + | |
| - | /*------------------------------------------------------------------------------------- Menu Bar */ | + | |
| - | div.MenuBar {
| + | |
| - | font: arial, helvetica, sans-serif;
| + | |
| - | height: 30px;
| + | |
| - | width: 910px;
| + | |
| - | /*width: 100%*/
| + | |
| - | margin: 0;
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| - | border-top: 0;
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| - | border-right: 0;
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| - | border-left: 0;
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| - | padding: 0;
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| - | background: black;
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| - |
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| - | }
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| - | div.MenuBar ul {
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| - | font: arial, helvetica, sans-serif;
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| - | text-align: center;
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| - | list-style-type: none;
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| - | border: 0;
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| - | padding: 0;
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| - | background: black;
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| - | div.MenuBar ul li {
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| - | font: arial, helvetica, sans-serif;
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| - | display: block;
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| - | padding: 0;
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| - | margin: 0;
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| - | font-size: 1.3em;
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| - | float: left;
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| - | background: black;
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| - | text-align: center;
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| - | width: 107px;
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| - | position: relative; /* Sets the positioning context for each drop-down menu. */
| + | |
| - | }
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| | | | |
| - | div.MenuBar ul li a {
| + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#Enrichment_cultures";>Enrichment Cultures</a></li> |
| - | font: arial, helvetica, sans-serif;
| + | |
| - | display: block;
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| - | background: black;
| + | |
| - | height: 22px; /* Keep height + padding-top + padding-bottom sync with the menu bar height. */
| + | |
| - | color: #ffffff;
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| - | padding-top: 4px;
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| - | padding-bottom: 4px;
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| - | padding-right: 1em; /* Sets the right space between top-level items. */
| + | |
| - | text-decoration: none;
| + | |
| - | }
| + | |
| | | | |
| - | /*------------------------------------------------------------------------------ Drop-Down Menus */
| + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#Rich_Media";>Rich Media</a></li> |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu {
| + | |
| - | display: block;
| + | |
| - | width: 10em; /* Drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | height: 1em;
| + | |
| - | padding: 1px; /* Sets the drop-down menu "effective border" width. */
| + | |
| - | position: absolute;
| + | |
| - | top: 23px; /* Places the drop-down menu under the menu bar.
| + | |
| - | Keep it sync with the menu bar height. */
| + | |
| - | left: 0; /* Aligns the drop-down menu to its top-level item. */
| + | |
| - | background-color: black; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| | | | |
| - | }
| + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#LB_plates";>LB Plates</a></li> |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| + | |
| - | width: 10em; /* Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | height: 1em;
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| - | padding-left: 0;
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| - | padding-right: 0;
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| - | background-color: black; /* Selected item. */
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| - | ul.DropDownMenu li a span {
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| + | |
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| + | |
| - | height: 20px;
| + | |
| - | color: #FFFFFF;
| + | |
| - | }
| + | |
| - | /*----------------------------------------------------------------------------------- Side Menus */
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
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| - | display: block;
| + | |
| - | width: 11em; /* Side menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | padding: 1px; /* Sets the side menu "effective border" width. */
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| - | position: absolute;
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| - | top: -1px; /* Aligns the side menu to its drop-down menu item.
| + | |
| - | Keep it sync with the side menu "effective border" width. */
| + | |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
| - | width: 11em; /* Keep it sync with the side menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
| + | |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
| + | |
| - | padding-left: 1.5em; /* Sets the left space of each side menu item. */
| + | |
| - | padding-right: 0.5em; /* Sets the right space of each side menu item. */
| + | |
| - | text-align: left;
| + | |
| - | font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
| + | |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | }
| + | |
| - | /*----------------------------------------------------------------------------- Browser Specific */
| + | |
| - | * html div.MenuBar ul li a {
| + | |
| - | float: left; /* Required for IE55 and IE6.
| + | |
| - | Breaks O9.
| + | |
| - | Hidden (* html) from non-IE browsers. */
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| - | }
| + | |
| - | * html ul.DropDownMenu li a:hover {
| + | |
| - | cursor: hand; /* Required for IE55.
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| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
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| - | ul.DropDownMenu li a:hover {
| + | |
| - | cursor: pointer; /* Required for IE6 and IE7.
| + | |
| - | Hidding it (* html) from non-IE browsers breaks IE7!
| + | |
| - | }
| + | |
| - | * html div.MenuBar a:hover {
| + | |
| - | text-decoration: none; /* Required for IE55 and IE6.
| + | |
| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
| + | |
| - | * html div.MenuBar ul li table,
| + | |
| - | * html div.MenuBar ul li table td {
| + | |
| - | border: 0; /* Required for IE55 and IE6.
| + | |
| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
| + | |
| - | /*------------------------------------------------------------------------------- Default Colors */
| + | |
| - | div.MenuBar {
| + | |
| - | background-color: Menu;
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| - | border-bottom: 1px solid ButtonShadow;
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| - | }
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| - | div.MenuBar a {
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| - | background-color: Menu; /* Top-level unselected items. */
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| - | color: MenuText;
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| - | div.MenuBar ul li:hover a,
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| - | div.MenuBar ul li a:hover {
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| - | color: #ea7f16;
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| - | background-color: Highlight; /* Top-level selected item. */
| + | |
| - | color: HighlightText;
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu {
| + | |
| - | background-color: ButtonShadow; /* Sets the drop-down menu "effective border" color. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| + | |
| - | background-color: Menu; Drop-down menu unselected items.
| + | |
| - | Sets the drop-down menu "effective background" color. */
| + | |
| - | color: MenuText;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
| + | |
| - | background-color: Highlight; /* Drop-down menu selected item. */
| + | |
| - | color: HighlightText;
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
| - | background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
| - | background-color: Menu; /* Side menu unselected items.
| + | |
| - | Sets the side menu "effective background" color. */
| + | |
| - | color: MenuText;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| + | |
| - | background-color: Highlight; /* Side menu selected item. */
| + | |
| - | color: HighlightText;
| + | |
| - | }
| + | |
| - | /*-----------------------------------------------------------------------------------------------*/
| + | |
| - | /*Script-Free 3-Level Menu 1.2 Tailor
| + | |
| - | www.CesarDaniel.info
| + | |
| - | /*-------------------------------------------------------------------------------------- General */
| + | |
| - | body {
| + | |
| - | background: orange;
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| - | color: black;
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| - | margin: 0;
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| - | border: 0;
| + | |
| - | padding: 0;
| + | |
| - | }
| + | |
| | | | |
| | + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#colony_PCR";>Colony PCR</a></li> |
| | | | |
| - | div.MenuBar {
| + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#:Electrophoresis";>Electrophoresis</a></li> |
| - | font: 13px arial, helvetica, sans-serif;
| + | |
| - | }
| + | |
| - | div.MenuBar ul {
| + | |
| - | font: 13px arial, helvetica, sans-serif; /* Required for IE55. */
| + | |
| - | }
| + | |
| - | /*--------------------------------------------------------------------------------------- Colors */
| + | |
| - | div.MenuBar {
| + | |
| - | background-color: black; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| - | border-bottom: 1px solid ButtonShadow;
| + | |
| - | }
| + | |
| - | div.MenuBar a,
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| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a,
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
| - | background-color: black; /* Selected item. */
| + | |
| - | color: #FFFFFF;
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| - | }
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| - | div.MenuBar ul li:hover a,
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| - | div.MenuBar ul li a:hover,
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| + | |
| - | background-color: #00b0e6; /* Selected item. */
| + | |
| - | color: #FFFFFF;
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| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu,
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
| - | background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
| + | |
| - | }
| + | |
| - | /*--------------------------------------------------------------------------------------- Widths */
| + | |
| - | /*
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| - | /*
| + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#:yeast_transformation";>Yeast Transformation</a></li> |
| - | Menu Bar 1
| + | |
| - | Drop-Down Menu #2
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| - | */
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4 li a {
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| - | width: 11em; /* Drop-down menu width. */
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
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| - | width: 12em; /* Drop-down menu width. */
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| - | /*...............................................................................................*/
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| - | /*
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| - | Menu Bar 1
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| - | Drop-Down Menu #2
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| - | */
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
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| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
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| - | Keep it sync with the drop-down menu width. */
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| - | }
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
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| - | width: 10em; /* Side menu width. */
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| - | }
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| - | /*...............................................................................................*/
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| - | /*
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| - | Menu Bar 1
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| - | Drop-Down Menu #2
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| - | Side Menu #2
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| - | */
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 {
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| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
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| - | Keep it sync with the drop-down menu width. */
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| - | }
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
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| - | width: 10em; /* Side menu width. */
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| - | }
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| - | /*...............................................................................................*/
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| - | /*
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| - | Menu Bar 1
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| - | Drop-Down Menu #2
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| - | Side Menu #3
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| - | */
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 {
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| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
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| - | Keep it sync with the drop-down menu width. */
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| - | }
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3,
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
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| - | width: 10em; /* Side menu width. */
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| - | }
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| - | /*...............................................................................................*/
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| | | | |
| - | </style> | + | <li><a href = "https://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#:Conjugation";>Conjugation</a></li> |
| | | | |
| | | | |
| - | <body>
| + | <li><a href="https://2012.igem.org/Team:Caltech/ Notebook/ material/ General_Protocols#Ethanol_Assay";> Ethanol Assay</a> </li> |
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| - |
| |
| - | ===Protocols===
| |
Here is a list of all the procedures we used this summer. Click on a protocol to read its description and the materials required.
"
