Team:Paris-Saclay/Project/Notebook

From 2012.igem.org

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<ol>
<ol>
<li>Sending of the B1 sample to sequencing with two pairs of primers</li>
<li>Sending of the B1 sample to sequencing with two pairs of primers</li>
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<li>
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<ol>
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<ol>Receipt of new primers
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<li>K-274100 Forward and Reverse</li>
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<li>Plasmid pSB1A2 Forward and Reverse</li>
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</ol>
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</ol></br>
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                              <p><b><i>7th august</i></b></p>
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<ol>
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<li>Liquid culture of B1 in order to prepare a glycerol stock</li>
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<li>Receipt of new primers</li>
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<ol>
<li>2 Forward</li>
<li>2 Forward</li>
<li>3 Reverse</li>
<li>3 Reverse</li>
<li>Plasmid Reverse</li>
<li>Plasmid Reverse</li>
</ol>
</ol>
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</li>
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</ol></br>
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</ol>
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<p><b><i>8th august</i></b></p>
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<p>Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.
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<img width="350px" src="https://static.igem.org/mediawiki/2012/c/c3/Week10-2.jpg"/>
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<img width="500px" src="https://static.igem.org/mediawiki/2012/8/8b/Week10-3.jpg"/>
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</p></br>
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<p><b><i>9th august</i></b></p>
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<p>New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before.
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<img width="350px" src="https://2012.igem.org/File:Week10-4.jpg"/>
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Digestion by EcoRI of the plasmid that contains BBa_K274100.
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</p></br>
                             </div>
                             </div>
                         </li>
                         </li>

Latest revision as of 06:46, 26 September 2012

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