Team:BostonU/Project Overview

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<ul id="nav">  
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<li><a href="https://2012.igem.org/Team:BostonU /Team">Team</a></li>  
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<li><a href="#">Our Team</a>
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<ul>
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<li><a href="https://2012.igem.org/Team:BostonU/Team">Team</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Social">Summer Fun</a></li>
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</li>
<li><a href="#">Project</a>  
<li><a href="#">Project</a>  
<ul>  
<ul>  
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<li><a href="https://2012.igem.org/Team:BostonU/Project_Overview">Overview</a></li>  
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<li><a href="https://2012.igem.org/Team:BostonU/Project_Overview">Project Overview and Abstract</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU /Characterization">Characterization</a></li>  
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                        <li><a href="https://2012.igem.org/Team:BostonU/MoClo2">Introduction to MoClo</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Characterization">Introduction to Characterization</a></li>
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                        <li><a href="https://2012.igem.org/Team:BostonU/DataSheet">Introduction to Data Sheets</a></li> 
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<li><a href="https://2012.igem.org/Team:BostonU/Methodology ">Methodology Overview</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Results ">Results Summary</a></li>
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<li><a href="#">Process</a>  
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<li><a href="#">Achievements</a>  
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<li><a href="https://2011.igem.org/Team:BU_Wellesley_Software/Methodology">Methodology</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Data">Data Collected</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU /Safety">Safety</a></li>  
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<li><a href="https://2012.igem.org/Team:BostonU/Parts">Parts Submitted</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU /Notebook">Notebook</a></li>  
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<li><a href="https://2012.igem.org/Team:BostonU/MoClo">MoClo Kit</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU /Human Practices">Human Practices</a></li>  
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<li><a href="https://2012.igem.org/Team:BostonU/Gold">Medal Fulfillment</a></li>  
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<li><a href="https://2012.igem.org/Team:BostonU /Tips">Tips and Tricks</a></li>
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<li><a href="2012.igem.org/Team:BostonU/Gold">Medal Fulfillment</a></li>
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<li><a href="#">Additional Info</a>
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<li><a href="#">Notebook</a>
<ul>
<ul>
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<li><a href="https://2012.igem.org/Team:BostonU/Acknowledgements">Acknowledgements</a></li>  
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<li><a href="https://2012.igem.org/Team:BostonU/Protocols">Protocols</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Social">Summer Fun</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Clotho">Clotho and Eugene</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Notebook">Weekly Notebook</a></li>
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</li>
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<li><a href="#">Considerations</a>
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<ul>
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<li><a href="https://2012.igem.org/Team:BostonU/NEGEM">New England iGEM Regional Meeting</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Human Practices">Human Practices</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Safety">Safety</a></li>
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</ul>
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<li><a href="#">Acknowledgements</a>
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<ul>
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                    <li><a href="https://2012.igem.org/Team:BostonU/Collaborations">Collaborations</a></li>
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                    <li><a href="https://2012.igem.org/Team:BostonU/Acknowledgements">Acknowledgements</a></li>
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                </ul>
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Our project has two major goals: 1. To introduce MoClo as an alternative assembly method for use by iGEM teams and 2. To develop a standard protocol for the characterization of genetic circuits containing fluorescent proteins and share this protocol with the synthetic biology community.
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<br><br>
 +
In order to achieve these goals, there many steps must be taken. First, we must convert BioBrick Parts into MoClo Parts using PCR. Upon converting basic parts, our project will have three thrusts:
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<br><br>
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<ul>
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<li><a href="https://2012.igem.org/Team:BostonU/MoClo2">Building:</a> Build Genetic Circuits with MoClo Parts
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<li><a href="https://2012.igem.org/Team:BostonU/Characterization">Characterizing:</a> Characterize Circuits using Flow Cytometry
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<li><a href="https://2012.igem.org/Team:BostonU/DataSheet">Sharing:</a> Generate Data Sheet for MoClo Parts</ul>
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<br>
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As we worked towards our first goal over the summer months, we ran into difficulties with some of our PCR and cloning reactions. This has unfortunately delayed our other goals, but we're still working hard towards generating a characterization workflow and a MoClo data sheet format for the Jamboree.
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<h4>Abstract</h4>
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<h9>Title</h9>
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<p dir="ltr">Abandon All Hope, Ye Who PCR: MoClo and the Quest for Genetic Circuit Characterization</h6>
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<h9>Authors</h9>
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<p dir="ltr">Monique De Freitas&#185;, Shawn Jin&#185;, Evan Appleton&#178;, Swati Carr&#178;, Sonya Iverson&#178;, Traci Haddock&#179;, and Douglas Densmore<SUP>&#167;</SUP></h7>
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<h9>Affiliations</h9>
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<p dir="ltr">&#185;iGEM Team Member, &#178;iGEM Team Mentor, &#179;iGEM Team Advisor, <SUP>&#167;</SUP>Faculty Sponsor,  Department of Electrical and Computer Engineering, Boston University, Boston, MA, USA</h7>
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<h9>Abstract</9>
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Our project has three aims: to introduce MoClo as an alternative assembly technique to BioBricks, to develop a standardized protocol for the characterization of genetic circuits using flow cytometry, and to share our MoClo Kit with the iGEM community. MoClo is an assembly technique developed by <a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0016765"> Weber et al., 2011</a>, which involves a multi-way, one-pot digestion-ligation reaction, enabling faster and more efficient construction of genetic circuits. We converted a large subset of BioBricks from the <a href="http://partsregistry.org/Main_Page">Registry</a> into MoClo Parts using PCR and cloning strategies. We are in the process of building and characterizing various genetic circuits using MoClo Parts, which we will compare to their BioBrick counterparts. A characterization workflow will be shared once this is complete. We also developed a data sheet using Clotho to be included in the Registry of Standard Biological Parts for each Part we characterized to easily share our data with the synthetic biology community.
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Latest revision as of 20:08, 25 October 2012

BostonU iGEM Team: Welcome


Project Overview


    Our project has two major goals: 1. To introduce MoClo as an alternative assembly method for use by iGEM teams and 2. To develop a standard protocol for the characterization of genetic circuits containing fluorescent proteins and share this protocol with the synthetic biology community.

    In order to achieve these goals, there many steps must be taken. First, we must convert BioBrick Parts into MoClo Parts using PCR. Upon converting basic parts, our project will have three thrusts:


    As we worked towards our first goal over the summer months, we ran into difficulties with some of our PCR and cloning reactions. This has unfortunately delayed our other goals, but we're still working hard towards generating a characterization workflow and a MoClo data sheet format for the Jamboree.

Abstract


Title

    Abandon All Hope, Ye Who PCR: MoClo and the Quest for Genetic Circuit Characterization


Authors

    Monique De Freitas¹, Shawn Jin¹, Evan Appleton², Swati Carr², Sonya Iverson², Traci Haddock³, and Douglas Densmore§


Affiliations

    ¹iGEM Team Member, ²iGEM Team Mentor, ³iGEM Team Advisor, §Faculty Sponsor, Department of Electrical and Computer Engineering, Boston University, Boston, MA, USA


Abstract

    Our project has three aims: to introduce MoClo as an alternative assembly technique to BioBricks, to develop a standardized protocol for the characterization of genetic circuits using flow cytometry, and to share our MoClo Kit with the iGEM community. MoClo is an assembly technique developed by Weber et al., 2011, which involves a multi-way, one-pot digestion-ligation reaction, enabling faster and more efficient construction of genetic circuits. We converted a large subset of BioBricks from the Registry into MoClo Parts using PCR and cloning strategies. We are in the process of building and characterizing various genetic circuits using MoClo Parts, which we will compare to their BioBrick counterparts. A characterization workflow will be shared once this is complete. We also developed a data sheet using Clotho to be included in the Registry of Standard Biological Parts for each Part we characterized to easily share our data with the synthetic biology community.



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