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Team:Trieste/notebook11
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<div id="suicide" class="notebook_section"> | <div id="suicide" class="notebook_section"> | ||
<h2 class="notebook_title">Suicide System</h2> | <h2 class="notebook_title">Suicide System</h2> | ||
| - | + | We tried to complete the construct, cloning the LL 37 construct inside the Holin ones, but unfortunately we didn't obtain positive colonies. | |
</div> | </div> | ||
<div id="antibody" class="notebook_section"> | <div id="antibody" class="notebook_section"> | ||
<h2 class="notebook_title">Antibody</h2> | <h2 class="notebook_title">Antibody</h2> | ||
| - | + | The hybridoma producing a new neutralizing scFv against norovirus arrived. We extracted its mRNA and recuperated its cDNA. We cloned VL and VH fragments into a sequencing plasmid and now we are waiting to the sequences to arrive. Our hope is to compare two scFv and determine which binds better norovirus VLPs. | |
</div> | </div> | ||
<div id="chassis" class="notebook_section"> | <div id="chassis" class="notebook_section"> | ||
| - | <h2 class="notebook_title"> | + | <h2 class="notebook_title">Cumate-Switch Regulation</h2> |
<u><b>CymR</b></u> | <u><b>CymR</b></u> | ||
<br/> | <br/> | ||
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<li><a href="https://2012.igem.org/Team:Trieste/notebook9">Week 9</a></li> | <li><a href="https://2012.igem.org/Team:Trieste/notebook9">Week 9</a></li> | ||
<li><a href="https://2012.igem.org/Team:Trieste/notebook10">Week 10</a></li> | <li><a href="https://2012.igem.org/Team:Trieste/notebook10">Week 10</a></li> | ||
| - | <li><a href="https://2012.igem.org/Team:Trieste/notebook11">Week 11</a></li> | + | <li class="select" ><a href="https://2012.igem.org/Team:Trieste/notebook11">Week 11</a></li> |
<li><a href="https://2012.igem.org/Team:Trieste/notebook12">Week 12</a></li> | <li><a href="https://2012.igem.org/Team:Trieste/notebook12">Week 12</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Trieste/notebooklast">Post European-Jamboree</a></li> | ||
</ul> | </ul> | ||
<img src="https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg" alt="Team iGEM 2012" id="igem_team" /> | <img src="https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg" alt="Team iGEM 2012" id="igem_team" /> | ||
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Follow us also: | Follow us also: | ||
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| - | <a href="https://twitter.com/ | + | <a href="https://twitter.com/iGEMTrieste" target="_blank"><img src="https://static.igem.org/mediawiki/2012/2/21/Ico_twitter.png" alt="twitter" class="tw" /></a> |
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 18:09, 26 October 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Week 11
More
Suicide System
We tried to complete the construct, cloning the LL 37 construct inside the Holin ones, but unfortunately we didn't obtain positive colonies.Antibody
The hybridoma producing a new neutralizing scFv against norovirus arrived. We extracted its mRNA and recuperated its cDNA. We cloned VL and VH fragments into a sequencing plasmid and now we are waiting to the sequences to arrive. Our hope is to compare two scFv and determine which binds better norovirus VLPs.Cumate-Switch Regulation
CymRWe tried several times to clone the single J23100-CymR-B0015 Eco/Pst into the plasmid K510012 but we didn't have positive colonies.
