Team:HKU HongKong/Safety.html
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- | <li class="topfirst"><a href="https://2012.igem.org/Team:HKU_HongKong" style="height:26px;line-height:26px;"> Home </a></li> | + | <li class="topfirst"><a href="https://2012.igem.org/Team:HKU_HongKong" style="height:26px;line-height:26px;"> |
- | <li class="topmenu"><a href="#" style="height:26px;line-height:26px;"> Team </a> | + | Home </a></li> |
+ | <li class="topmenu"><a href="#" style="height:26px;line-height:26px;"> | ||
+ | Team </a> | ||
<ul> | <ul> | ||
- | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Team/About_Us.html">About Us</a></li></font> | + | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Team/About_Us.html"> |
- | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Team/Profiles.html">Profiles</a></li></font> | + | About Us</a></li></font> |
+ | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Team/Profiles.html"> | ||
+ | Profiles</a></li></font> | ||
</ul> | </ul> | ||
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- | <li class="topmenu"><a href="#" style="height:26px;line-height:26px;"> Project </a> | + | <li class="topmenu"><a href="#" style="height:26px;line-height:26px;"> |
+ | Project </a> | ||
<ul> | <ul> | ||
- | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Project/Background.html">Background</a></li></font> | + | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Project/Background.html"> |
- | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Project/Future_Implications.html">Future Implications</a></li></font> | + | Background</a></li></font> |
+ | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Project/Future_Implications.html"> | ||
+ | Future Implications</a></li></font> | ||
</ul> | </ul> | ||
</li> | </li> | ||
- | <li class="topmenu"><a href="#" style="height:26px;line-height:26px;"> Data </a> | + | <li class="topmenu"><a href="#" style="height:26px;line-height:26px;"> |
+ | Data </a> | ||
<ul> | <ul> | ||
- | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/Weekly_Notebook.html">Weekly Notebook</a></li></font> | + | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/Weekly_Notebook.html"> |
- | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/Bio_Bricks.html">Bio Bricks</a></li></font> | + | Weekly Notebook</a></li></font> |
- | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/Results.html">Results</a></li></font> | + | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/Bio_Bricks.html"> |
+ | Bio Bricks</a></li></font> | ||
+ | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/Results.html"> | ||
+ | Results</a></li></font> | ||
</ul> | </ul> | ||
</li> | </li> | ||
- | <li class="topmenu"><a href="#" style="height:26px;line-height:26px;"> Protocols </a> | + | <li class="topmenu"><a href="#" style="height:26px;line-height:26px;"> |
+ | Protocols </a> | ||
<ul> | <ul> | ||
- | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/Mol_Protocols.html"> Molecular Cloning </a></li></font> | + | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/Mol_Protocols.html"> |
- | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/pvdQ_Protocols.html"> pvdQ Expression Analysis </a></li></font> | + | Molecular Cloning </a></li></font> |
+ | <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/pvdQ_Protocols.html"> | ||
+ | pvdQ Expression Analysis </a></li></font> | ||
</ul> | </ul> | ||
</li> | </li> | ||
- | <li class="topmenu"><a href="https://2012.igem.org/Team:HKU_HongKong/Safety.html" style="height:26px;line-height:26px;"> Safety </a></li> | + | <li class="topmenu"><a href="https://2012.igem.org/Team:HKU_HongKong/Safety.html" style="height:26px;line-height:26px;"> |
- | + | Safety </a></li> | |
- | <li class="toplast"><a href="https://2012.igem.org/Team:HKU_HongKong/Human_Practice.html" style="height:26px;line-height:26px;"> Human Practice </a></li> | + | <li class="toplast"><a href="https://2012.igem.org/Team:HKU_HongKong/Human_Practice.html" style="height:26px;line-height:26px;"> |
+ | Human Practice </a></li> | ||
</ul> | </ul> | ||
<p> </p> | <p> </p> | ||
<h2 style="font-variant: normal; vertical-align: baseline; clear: left; color: #232323; font-family: Gentium Basic; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin: 0.7em 0px; padding: 0px"> | <h2 style="font-variant: normal; vertical-align: baseline; clear: left; color: #232323; font-family: Gentium Basic; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin: 0.7em 0px; padding: 0px"> | ||
- | <span style="font-weight: 400; | + | <span style="font-weight: 400; "> |
- | <font face="Trebuchet MS" size="6">Safety</font></span></h2> | + | <font face="Trebuchet MS" size="6"><u>Safety</u><br> |
- | <p style="text-align: left; font-style: | + | </font></span></h2> |
- | Members of our IGEM team members were required to attend a pre-lab training offered by the Department of Biochemistry, the University of Hong Kong before gaining access to the labs. The training was divided into 2 sessions, including safety talk and laboratory inspection, which rise awareness of any potential dangers in the lab to our team members. The Department of Biochemistry has provided and maintained a safe environment, in compliance with the Occupational Safety and Health Regulations for every team member. </p> | + | <p style="text-align: left; font-style: italic; font-variant: normal; font-weight: normal; vertical-align: baseline; color: #232323; font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> |
+ | <font size="3">Members of our IGEM team members were required to attend | ||
+ | a pre-lab training offered by the Department of Biochemistry, the | ||
+ | University of Hong Kong before gaining access to the labs. The training | ||
+ | was divided into 2 sessions, including safety talk and laboratory | ||
+ | inspection, which rise awareness of any potential dangers in the lab to | ||
+ | our team members. The Department of Biochemistry has provided and | ||
+ | maintained a safe environment, in compliance with the Occupational | ||
+ | Safety and Health Regulations for every team member. </font> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 16px; vertical-align: baseline; color: #232323; font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <br> | ||
+ | <b>1. Would any of your project ideas raise safety issues in terms of: | ||
+ | </b> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: 700; font-size: 14px; vertical-align: baseline; color: #232323; font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | a) Researcher safety </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: #232323; font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | No, as we have series of measure to team members. Laboratory inspection | ||
+ | along with a safety talk held by safety chief is required, which | ||
+ | strengthen the awareness of laboratory safety. Moreover, information | ||
+ | pamphlets on safety issue have been distributed to all of our team | ||
+ | members as a guide. Nevertheless, supervision from advisor is required | ||
+ | in the department. This provides better protection towards our team | ||
+ | members. </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: #232323; font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | Before using new chemicals or bacterial stain in lab, Material Safety | ||
+ | Data Sheet or Biosafety Level of particular stain was checked to ensure | ||
+ | if there are any potential dangers to researcher. </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: 700; font-size: 14px; vertical-align: baseline; color: #232323; font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | b) Public safety </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: #232323; font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | In general, we will be using E.coli strain DH5a to conduct plasmid | ||
+ | engineering. These strains of E. coli are not known to cause harm to | ||
+ | humans as well as animals. According to the Biosafety manual of the | ||
+ | World Health Organisation, these groups of bacteria belong to Biosafety | ||
+ | Level 1. For isolation of pvdQ, genomic DNA of P. aerugonosa strain | ||
+ | Boston 41501 is used, in which it belongs to Biosafety Level 1, instead | ||
+ | of extracted from P. aerugonosa, which is Biosafety Level 2. </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323"> http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=27853&Template=bacteria </font> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323"> http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=27853D-5&Template=bacteria </font> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323"> To avoid the spread of E.coli, used agar plates, | ||
+ | eppendorf tubes, and other laboratory apparatus were disposed in | ||
+ | separate containers and sterilize them by autoclave before disposal. | ||
+ | Thus, bacteria can hardly be dispersed and harm public. </font> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: 700; font-size: 14px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323"> c) Environmental safety </font> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323">Apart from bacterial waste treatment mentioned in | ||
+ | public safety, our lab bench was cleaned by 70% ethanol before we leave | ||
+ | to prevent contamination. Every team members will clean their hands | ||
+ | before they leave lab to prevent contaminate external environment by our | ||
+ | experiment bacteria. For AHL waste produced from lab work, it was | ||
+ | labeled and treated separately to prevent pollution to external | ||
+ | environment. </font> </p> | ||
+ | <p style="text-align: left; font-style: italic; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323"> - Do any of the new BioBrick parts (or devices) | ||
+ | that you made this year raise any safety issues? </font> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323"> No. There are no safety issues raised by the new | ||
+ | BioBrick parts because they do not pose any risk to humans as well as | ||
+ | the environment. </font> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: 700; font-size: 12pt; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323">2. Is there a local biosafety group, committee, or | ||
+ | review board at your institution? If yes, what does your local biosafety | ||
+ | group think about your project? </font> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323"> Yes! The Safety Committee of Department of | ||
+ | Biochemistry in the University of Hong Kong. We discuss the biosafety | ||
+ | aspect with the safety committee members at every stage of our progress. | ||
+ | The committee concerns safety issues in three areas in particular: | ||
+ | general, biological and radiation. Each area is under the supervision of | ||
+ | different safety officers. After review from the Safety Committee, they | ||
+ | think the project is safe to work by undergraduate as there is | ||
+ | supervision from advisor in iGEM team. </font> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: 700; font-size: 16px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323">3. Do you have any other ideas how to deal with | ||
+ | safety issues that could be useful for future iGEM competitions? How | ||
+ | could parts, devices and systems be made even safer through biosafety | ||
+ | engineering? </font> </p> | ||
+ | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px"> | ||
+ | <font color="#232323"> We would like to suggest to assign a particular | ||
+ | area on lab bench only for microbe work. This area can reduce chance of | ||
+ | contamination by microbe in two ways. First, only microbes are allowed | ||
+ | to work in particular area means less area in the lab will be | ||
+ | contaminated. </font> | ||
+ | </p> | ||
+ | </object> | ||
+ | </font> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 03:23, 27 September 2012