Team:LMU-Munich/Lab Notebook/Protocols

From 2012.igem.org

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==Protocols==
 
On this page, we offer our unique protocols to the public.
On this page, we offer our unique protocols to the public.
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PCR protocol for long flanking homology PCR. You might need those PCR products for interrupting genes with resistance cassettes.
PCR protocol for long flanking homology PCR. You might need those PCR products for interrupting genes with resistance cassettes.
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[https://static.igem.org/mediawiki/2012/6/67/LMU-Munich_2012_Removal_of_germination_genes.pdf Example: Using LFH PCR to replace germination genes with resistance cassettes]
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An explanation of how we used this protocol for how we knocked out our germination genes using LFH.
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9. Mikroskopie der Sporen
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9. [https://static.igem.org/mediawiki/2012/e/e9/LMU-Munich_2012_Protocol_for_enhancement_of_mature_spore_numbers.pdf Enhancement of mature spore numbers]
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Protocol to enrich the spore numbers and purification from vegetative cells.
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11. Plate reader assay (can also be used with E. coli cells)
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11. [https://static.igem.org/mediawiki/2012/e/e6/LMU-Munich_2012_Protocol_Plate_Reader.pdf Luminescence measurement]
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This protocol explains how cultures were treated in advance of a plate reader assay.
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[http://openwetware.org/wiki/Silver:_Restriction_Digest Ligation]
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[http://openwetware.org/wiki/Silver:_Restriction_Digest Restriction digest]
[http://openwetware.org/wiki/Silver:_Ligation Ligation]
[http://openwetware.org/wiki/Silver:_Ligation Ligation]
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https://static.igem.org/mediawiki/2012/b/bb/LMU-Munich_2012_%C3%9F-Galactosidase_Assay_E._coli.pdf %C3%9F-Galactosidase Assay in ''E. coli'']
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[https://static.igem.org/mediawiki/2012/b/bb/LMU-Munich_2012_%C3%9F-Galactosidase_Assay_E._coli.pdf β-Galactosidase Assay in ''E. coli'']
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''lacZ''-assay in ''E.coli''
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Quantitative ''lacZ''-assay in ''E.coli''.
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Latest revision as of 02:00, 27 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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