Team:Exeter/lab book/1gp/wk7

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    <!--Project Division Week Hyperlinks-->
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    <!--Project Division Week Hyperlinks-->
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         <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk8"; style="color:#1d1d1b">27th - 31st August</a>
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         <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk11"; style="color:#1d1d1b">17th - 21st September</a>
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        <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk9"; style="color:#1d1d1b">3rd - 7th September</a>
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        <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk10"; style="color:#1d1d1b">10th - 14th September</a>
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         <p>
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         -
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         <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk11"; style="color:#1d1d1b">17th - 21st September</a>
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         <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk12"; style="color:#1d1d1b">24th - 28th September</a>
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         <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk12"; style="color:#1d1d1b">24th - 28th September</a></font>
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         <a href="https://2012.igem.org/Team:Exeter/Results/characterise"; style="color:#e30614" target="_blank"><font size="3"><b>Results</b></font></a>
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     <p><u><b>Monday 20th August (11.00)</u></b></p>
     <p><u><b>Monday 20th August (11.00)</u></b></p>
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<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#1d1d1b"><u>3A assembly</u></a> of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3</p>
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<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#57b947"><u>3A assembly</u></a> of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3</p>
<p><u><b>Tuesday 21st August (9.00)</u></b></p>
<p><u><b>Tuesday 21st August (9.00)</u></b></p>
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<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#1d1d1b"><u>Transformation</u></a> of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3</p>
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<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#57b947"><u>Transformation</u></a> of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3</p>
<p><u><b>Wednesday 22nd August (9.00)</u></b></p>
<p><u><b>Wednesday 22nd August (9.00)</u></b></p>
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<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#1d1d1b"><u>3A assembly</u></a> of WclY-pSB1C3 and WbiP-pSB1C3</p>
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<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#57b947"><u>3A assembly</u></a> of WclY-pSB1C3 and WbiP-pSB1C3</p>
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<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#1d1d1b"><u>Adding cultures</u></a> with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight</p>
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<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#57b947"><u>Adding cultures</u></a> with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight</p>
<p> No colonies with ligated WclY-pSB1C3 and WbiP-pSB1C3 were found and thus 3A assembly was repeated.
<p> No colonies with ligated WclY-pSB1C3 and WbiP-pSB1C3 were found and thus 3A assembly was repeated.
<p><u><b>Thursday 23rd August (9.00)</u></b></p>
<p><u><b>Thursday 23rd August (9.00)</u></b></p>
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<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#1d1d1b"><u>Adding cultures</u></a> with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight</p>
+
<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#57b947"><u>Adding cultures</u></a> with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight</p>
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<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#1d1d1b"><u>Transformation</u></a> of WclY-pSB1C3 and WbiP-pSB1C3</p>
+
<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#57b947"><u>Transformation</u></a> of WclY-pSB1C3 and WbiP-pSB1C3</p>
<p> The shaking incubator broke on Wednesday evening and therefore adding cultures into liquid medium was repeated.
<p> The shaking incubator broke on Wednesday evening and therefore adding cultures into liquid medium was repeated.
      
      
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    <p><u>Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley</u> &nbsp;&nbsp;|&nbsp;&nbsp;
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    <a href="https://igem.org/Team.cgi?id=764" style="color:#57B947" target="_blank"><u>Contact Us</u></a>  &nbsp;&nbsp;|&nbsp;&nbsp;
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    <a href="https://2012.igem.org/Team:Exeter/site_map" style="color:#57B947"><u>Site Map</u></a></p>
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Latest revision as of 23:55, 26 September 2012

ExiGEM2012 Lab Book 1GP wk7

Single Gene Plasmids and Enzyme Characterisation: 20th - 24th August 2012

Monday 20th August (11.00)

• 3A assembly of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3

Tuesday 21st August (9.00)

• Transformation of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3

Wednesday 22nd August (9.00)

• 3A assembly of WclY-pSB1C3 and WbiP-pSB1C3

• Adding cultures with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight

No colonies with ligated WclY-pSB1C3 and WbiP-pSB1C3 were found and thus 3A assembly was repeated.

Thursday 23rd August (9.00)

• Adding cultures with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight

• Transformation of WclY-pSB1C3 and WbiP-pSB1C3

The shaking incubator broke on Wednesday evening and therefore adding cultures into liquid medium was repeated.

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