Team:Exeter/Achievements

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   <font color="#57B947" size="+2" face="Verdana">Achievements and Failures</font>
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   <font color="#57B947" size="+2" face="Verdana">Achievements</font>
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    <p><b>Achievements</b></p>
 
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Successfully cloned and submitted 32 biobricks into the registry</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Successfully cloned and submitted 14 biobricks into the registry</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Obtained WbbC gene from BL21 genome</li>
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<li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Successfully cloned over 32 more Biobricks waiting to be transferred into pSB1C3 plasmids</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoBase; a database containing a list of >100 glycosyltransferase enzymes  
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Using PCR we obtained <i>wbbC</i> gene from BL21 genome</li>
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     from <i>E.coli</i> strains to demonstrate the vast linkages we could achieve only with <i>E.coli</i> enzymes</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoBase; a database containing a list of over 100 different glycosyltransferase enzymes mainly
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoWeb, an interface for an online user to access Glycobase with a
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     from <i>E.coli</i> strains demonstrating the vast linkages we could achieve with just <i>E.coli</i> enzymes</li>
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    polysaccharide in mind and the database searches and finds the enzymes you’d use to make it.</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoWeb, an interface for an online user to access Glycobase. Glycoweb has the ability to tell you which enzymes are needed to create your bespoke polysaccharide.</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoApp; an application for a handheld device to access the Glycobase from
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Created GlycoApp; an application for a handheld device to access the Glycobase giving the user distance access and enhanced ordering capabilities</li>
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    wherever the consumer is when they realise they need a polysaccharide ordering!</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">The project evolved with human practices for the consideration of the ethical, societal, environmental and business aspects of our project. We held a human practice panel, Café Scientifique talk, met with various company members from different business sectors, led an A level master class and worked with several work experience students.</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Considered human practice approaches and discussed with several experts the human  
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">The discussions with various businesses had a very positive response and we received several letters of support.</li>
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    practice considerations, hosted a human practice panel and discussed with the wider community at Café Scientifique.</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">We created devices to test our proteins, test our natural polysaccharides and to help construct our 3 gene inducible plasmid. These included BBa_K094120_BBa_B0034_<i>wclY</i>_BBaB0014, BBa_J13002_<i>wbnk</i>_BBaB0014, BBa_J23119_BBa_B0034_ <i>wbnJ</i>_BBaB0014 BBa_J13002_ompA_BBa_J322921_BBa_B0034 and BBa_K20600_ompA_BBa_J322921_BBa_B0034.
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Discussed our project with various businesses including Shell, Ginsters etc. and we  
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</li>
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    received letters of support from DSTL, ISCA Biochemical Ltd, Avon. </li>
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<li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">We managed to obtain 7 of 9 fragments for Gibson assembly through PCR. The different annealing temperatures of the primers made this hard but showed how important the preparation for Gibson has to be.</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Demonstrated that….. Successfully expressed this polysaccharide….. (to be cont….)</li>
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    <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/9/99/Exe2012Tick.jpg);">Made promoter-RBS-gene-terminator constructs for each gene</li>
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    <p><b>Failures</b></p>
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<p><font color="#57B947" size="+2" face="Verdana">Nearly There!!</font></p>
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    <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">A correct mass analysis of the product is essential, but cannot alone confirm the
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     <ul>  
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    correct product. The possibility that an incorrect product has been made through incorrect synthesis or post-synthetic regiospecific or enantiospecific rearrangement by
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<li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">In touching distance of completing our operons. Only time stopped this happening.</li>
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    chemical or enzymatic means must be examined and ruled out before the method can be considered reliable.</li>
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<li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">We were one construct away from being able to complete our 3 gene inducible plasmid.</li>
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    <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">Synthesis of a previously well characterised small polysaccharide, which can be
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    physically and also biochemically characterised, to show that the product will act in biological systems as expected. Once a fully characterised polysaccharide is produced, we
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<li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">We were not able to fully test our constructs and properly characterise our biobricks.</li>
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    would be in a much stronger position to offer novel products and to start to address the exciting possibilities of our method.</li>
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     <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">Only managed to obtain 7 of 9 fragments for Gibson assembly through PCR.</li>
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    <li style="list-style-image: url(https://static.igem.org/mediawiki/2012/1/11/Exe2012Cross.png);">Didn’t manage to construct operons through BioBrick assembly.</li>
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  <font face="Verdana" color="#57b947" size="3"><a href="https://2012.igem.org/Team:Exeter/Results"; style="color:#57b947"><u><< Return to Results</u></a>
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  <font face="Verdana" color="#57b947" size="3"><a href="https://2012.igem.org/Team:Exeter/Outreach"; style="color:#57b947"><u>See Our Outreach Programme >></u></a>
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    <p><u>Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley</u> &nbsp;&nbsp;|&nbsp;&nbsp;
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    <a href="https://igem.org/Team.cgi?id=764" style="color:#57B947" target="_blank"><u>Contact Us</u></a>  &nbsp;&nbsp;|&nbsp;&nbsp;
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    <a href="https://2012.igem.org/Team:Exeter/site_map" style="color:#57B947"><u>Site Map</u></a></p>
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Latest revision as of 02:39, 27 September 2012

Protocol 6

Achievements
  • Successfully cloned and submitted 14 biobricks into the registry
  • Successfully cloned over 32 more Biobricks waiting to be transferred into pSB1C3 plasmids
  • Using PCR we obtained wbbC gene from BL21 genome
  • Created GlycoBase; a database containing a list of over 100 different glycosyltransferase enzymes mainly from E.coli strains demonstrating the vast linkages we could achieve with just E.coli enzymes
  • Created GlycoWeb, an interface for an online user to access Glycobase. Glycoweb has the ability to tell you which enzymes are needed to create your bespoke polysaccharide.
  • Created GlycoApp; an application for a handheld device to access the Glycobase giving the user distance access and enhanced ordering capabilities
  • The project evolved with human practices for the consideration of the ethical, societal, environmental and business aspects of our project. We held a human practice panel, Café Scientifique talk, met with various company members from different business sectors, led an A level master class and worked with several work experience students.
  • The discussions with various businesses had a very positive response and we received several letters of support.
  • We created devices to test our proteins, test our natural polysaccharides and to help construct our 3 gene inducible plasmid. These included BBa_K094120_BBa_B0034_wclY_BBaB0014, BBa_J13002_wbnk_BBaB0014, BBa_J23119_BBa_B0034_ wbnJ_BBaB0014 BBa_J13002_ompA_BBa_J322921_BBa_B0034 and BBa_K20600_ompA_BBa_J322921_BBa_B0034.
  • We managed to obtain 7 of 9 fragments for Gibson assembly through PCR. The different annealing temperatures of the primers made this hard but showed how important the preparation for Gibson has to be.

Nearly There!!


  • In touching distance of completing our operons. Only time stopped this happening.
  • We were one construct away from being able to complete our 3 gene inducible plasmid.
  • We were not able to fully test our constructs and properly characterise our biobricks.
<< Return to Results See Our Outreach Programme >>

Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley   |   Contact Us   |   Site Map

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