"
Team:Hong Kong-CUHK/DOC NBK JUL
From 2012.igem.org
(Difference between revisions)
Kpsleung1990 (Talk | contribs) |
|||
| (2 intermediate revisions not shown) | |||
| Line 7: | Line 7: | ||
<td style="background-color:#FFF; padding:25px"><!-- InstanceBeginEditable name="EditRegion1" --><p> </p> | <td style="background-color:#FFF; padding:25px"><!-- InstanceBeginEditable name="EditRegion1" --><p> </p> | ||
| - | + | <p class="aloveofthunder" style="line-height:normal; margin-bottom:35px">NOTEBOOK - JULY</p> | |
| - | <p class="aloveofthunder" style="line-height:normal">NOTEBOOK - JULY</p> | + | |
<ol> | <ol> | ||
<li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | <li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | ||
| - | + | </br> | |
<li>Plasmids containing NpSRII-NpHtrII-Ectar -backbone gene were sent to sequencing. </li> | <li>Plasmids containing NpSRII-NpHtrII-Ectar -backbone gene were sent to sequencing. </li> | ||
<li>Make plate with 0.5% soft agar with antibiotics- chloramphenicol. </li> | <li>Make plate with 0.5% soft agar with antibiotics- chloramphenicol. </li> | ||
<li>Make plate with antibiotics –Ampiclin. </li> | <li>Make plate with antibiotics –Ampiclin. </li> | ||
<li>Overlapping PCR of NpSRII-NpHtrII-Ectsr-backbone which the plasmid is responsible in repelling from blue light.</li> | <li>Overlapping PCR of NpSRII-NpHtrII-Ectsr-backbone which the plasmid is responsible in repelling from blue light.</li> | ||
| - | + | </br> | |
<li>Direct transformation of NpSRII-NpHtrII-Ectsr-backbone gene, follow by spread plate. </li> | <li>Direct transformation of NpSRII-NpHtrII-Ectsr-backbone gene, follow by spread plate. </li> | ||
<li>Miniprep of picked clones from transformed HsSRI-HsHtrI-Ectsr-backbone cells. </li> | <li>Miniprep of picked clones from transformed HsSRI-HsHtrI-Ectsr-backbone cells. </li> | ||
<li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-backbone gene are incorrect. </li> | <li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-backbone gene are incorrect. </li> | ||
| - | + | </br> | |
<li>Overlapping PCR of NpSRII-NpHtrII-Ectar -backbone which the plasmid is responsible in attracting by blue light.</li> | <li>Overlapping PCR of NpSRII-NpHtrII-Ectar -backbone which the plasmid is responsible in attracting by blue light.</li> | ||
<li>Make LB agar solution</li> | <li>Make LB agar solution</li> | ||
| - | + | </br> | |
<li>Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate. </li> | <li>Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate. </li> | ||
<li>No clone was being found on the plate containing transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | <li>No clone was being found on the plate containing transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | ||
| - | + | </br> | |
<li>Overlapping PCR of NpSRII-NpHtrII-Ectar-backbone which the plasmid is responsible in attracting by blue light.</li> | <li>Overlapping PCR of NpSRII-NpHtrII-Ectar-backbone which the plasmid is responsible in attracting by blue light.</li> | ||
<li>Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate. </li> | <li>Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate. </li> | ||
<li>Pick clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | <li>Pick clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | ||
<li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | <li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | ||
| - | + | </br> | |
<li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells and was sent for sequencing. </li> | <li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells and was sent for sequencing. </li> | ||
<li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar -backbone gene is correct. </li> | <li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar -backbone gene is correct. </li> | ||
<li>The sequenced cells which contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics. </li> | <li>The sequenced cells which contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics. </li> | ||
| - | + | </br> | |
<li>Repeat in testing cells that contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics. </li> | <li>Repeat in testing cells that contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics. </li> | ||
<li>Pick clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells. </li> | <li>Pick clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells. </li> | ||
Latest revision as of 09:03, 26 September 2012
 |
Check out our FACEBOOK page! |
|
|
NOTEBOOK - JULY
|
Home | Team | Project | Biobricks | Human Practice | Safety | Documentation | Acknowledgement
Address: Rm. 184, Science Centre, CUHK Copyright © 2012 Apycom jQuery Menus |
