Team:Hong Kong-CUHK/DOC NBK JUL

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<td style="background-color:#FFF; padding:25px"><!-- InstanceBeginEditable name="EditRegion1" --><p>&nbsp;</p>
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      <p class="aloveofthunder" style="line-height:normal; margin-bottom:35px">NOTEBOOK - JULY</p>
 +
      <ol>
 +
        <li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li>
 +
        </br>
 +
        <li>Plasmids containing NpSRII-NpHtrII-Ectar -backbone gene were sent to sequencing. </li>
 +
        <li>Make plate with 0.5% soft agar with antibiotics- chloramphenicol. </li>
 +
        <li>Make plate with antibiotics –Ampiclin. </li>
 +
        <li>Overlapping PCR of NpSRII-NpHtrII-Ectsr-backbone which the plasmid is responsible in repelling from blue light.</li>
 +
        </br>
 +
        <li>Direct transformation of NpSRII-NpHtrII-Ectsr-backbone gene, follow by spread plate. </li>
 +
        <li>Miniprep of picked clones from transformed HsSRI-HsHtrI-Ectsr-backbone cells. </li>
 +
        <li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-backbone gene are incorrect. </li>
 +
        </br>
 +
        <li>Overlapping PCR of NpSRII-NpHtrII-Ectar -backbone which the plasmid is responsible in attracting by blue light.</li>
 +
        <li>Make LB agar solution</li>
 +
        </br>
 +
        <li>Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate. </li>
 +
        <li>No clone was being found on the plate containing transformed NpSRII-NpHtrII-Ectar-backbone cells. </li>
 +
        </br>
 +
        <li>Overlapping PCR of NpSRII-NpHtrII-Ectar-backbone which the plasmid is responsible in attracting by blue light.</li>
 +
        <li>Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate. </li>
 +
        <li>Pick clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li>
 +
        <li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li>
 +
        </br>
 +
        <li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells and was sent for sequencing. </li>
 +
        <li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar -backbone gene is correct. </li>
 +
        <li>The sequenced cells which contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics. </li>
 +
        </br>
 +
        <li>Repeat in testing cells that contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar     plate for testing using normal light bulb with its corresponding antibiotics. </li>
 +
        <li>Pick clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells. </li>
 +
        <li>Plasmids containing HsSRI-HsHtrI-Ectsr -backbone gene were sent to sequencing. </li>
 +
        <li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells. </li>
 +
      </ol>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
    <!-- InstanceEndEditable --></td>

Latest revision as of 09:03, 26 September 2012



 

 

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NOTEBOOK - JULY

  1. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells.

  2. Plasmids containing NpSRII-NpHtrII-Ectar -backbone gene were sent to sequencing.
  3. Make plate with 0.5% soft agar with antibiotics- chloramphenicol.
  4. Make plate with antibiotics –Ampiclin.
  5. Overlapping PCR of NpSRII-NpHtrII-Ectsr-backbone which the plasmid is responsible in repelling from blue light.

  6. Direct transformation of NpSRII-NpHtrII-Ectsr-backbone gene, follow by spread plate.
  7. Miniprep of picked clones from transformed HsSRI-HsHtrI-Ectsr-backbone cells.
  8. Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-backbone gene are incorrect.

  9. Overlapping PCR of NpSRII-NpHtrII-Ectar -backbone which the plasmid is responsible in attracting by blue light.
  10. Make LB agar solution

  11. Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate.
  12. No clone was being found on the plate containing transformed NpSRII-NpHtrII-Ectar-backbone cells.

  13. Overlapping PCR of NpSRII-NpHtrII-Ectar-backbone which the plasmid is responsible in attracting by blue light.
  14. Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate.
  15. Pick clones from transformed NpSRII-NpHtrII-Ectar-backbone cells.
  16. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells.

  17. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells and was sent for sequencing.
  18. Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar -backbone gene is correct.
  19. The sequenced cells which contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics.

  20. Repeat in testing cells that contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar     plate for testing using normal light bulb with its corresponding antibiotics.
  21. Pick clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells.
  22. Plasmids containing HsSRI-HsHtrI-Ectsr -backbone gene were sent to sequencing.
  23. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells.

 

 

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