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Team:Trieste/parts
From 2012.igem.org
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<div id="box_main"> <!-- start box_main --> | <div id="box_main"> <!-- start box_main --> | ||
<div class="box_contenuti"> | <div class="box_contenuti"> | ||
| - | <h2>List of the | + | <h2>List of the Submitted Parts </h2> |
| - | <h3>Click on the code of each part to have more information about the Biobrick | + | <h3>Click on the code of each part to have more information about the Biobrick, its construction, the results obtained and the relative link to the Registry.</h3> |
</br> | </br> | ||
</br> | </br> | ||
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<td><strong>Type</strong></td> | <td><strong>Type</strong></td> | ||
<td></td> | <td></td> | ||
| - | <td><strong> | + | <td><strong>Description</strong></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>Regulatory</td> | <td>Regulatory</td> | ||
<td></td> | <td></td> | ||
| - | <td>T5 Lac | + | <td>T5 Lac Operator</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 95: | Line 95: | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
| - | </center> | + | </center> |
| + | <br/> | ||
| + | <h2>Primers </h2> | ||
| + | <table> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th> Name</th> | ||
| + | <th> Seq 5'--> 3'</th> | ||
| + | <th> PCR Notes</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | |||
| + | <tbody> | ||
| + | <tr><td>Vr</td> <td>ATTACCGCCTTTGAGTGAGC</td> <td>95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Vf2</td> <td>TGCCACCTGACGTCTAAGAA</td> <td> 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>GlmSTn7Fw</td> <td>GCATGTGGAAGAGGTGATTG</td> <td>95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr> | ||
| + | <tr><td>GlmSTn7Rev</td> <td>ACTTTATTGTTCGCCCACA</td> <td>95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr> | ||
| + | <tr><td>B0015XhoRev</td> <td>ACTCGAGATTACTAGTTATAAACGCAGAAAGGCCCACCC</td> <td>95° 5' | 30x (93° 30'' | 58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr> | ||
| + | <tr><td>B0015EcoFw</td> <td>CGAATTCATCAGATCTCCAGGCATCAAATAAAACGAAAGG</td> <td>95° 5' | 30x (93° 30'' |58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr> | ||
| + | <tr><td>T5EcoFw</td> <td>CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT</td> <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>T5XhoRev</td> <td>TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA</td> <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta1/2FW</td> <td>ATACTACGACGGTAAATGGT</td> <td>95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta1/2REV</td> <td>TACATCAGTATCGGTAGCAT</td> <td>95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta7/8FW</td> <td>GACCAAGCGATAACCGGATG</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta7/8REV</td> <td>GTGAGATGATGGCCACGATT</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta9/10FW</td> <td>GCGAGGTAACCTCGAACATG</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta9/10REV</td> <td>CGGCGTATCGATAATTCACG</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | |||
<!-- ******************** CONTENUTO QUI ****************** --> | <!-- ******************** CONTENUTO QUI ****************** --> | ||
</div> | </div> | ||
</div> <!-- end box_main --> | </div> <!-- end box_main --> | ||
<div id="box_right"> <!-- start box_right --> | <div id="box_right"> <!-- start box_right --> | ||
| - | <ul id="sub_menu"> | + | <ul id="sub_menu"><strong> |
| - | <li><a href="https://2012.igem.org/Team:Trieste/parts/1">BBa_K875001</a></li> | + | <li class="select"><a href="https://2012.igem.org/Team:Trieste/parts">Overwiev</a></li> |
| - | <li><a href="https://2012.igem.org/Team:Trieste/parts/2">BBa_K875002</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/1">BBa_K875001 - Cumate Op</a></li> |
| - | <li><a href="https://2012.igem.org/Team:Trieste/parts/3">BBa_K875003</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/2">BBa_K875002 - Lac OP</a></li> |
| - | <li><a href="https://2012.igem.org/Team:Trieste/parts/4">BBa_K875004</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/3">BBa_K875003 - CymR</a></li> |
| - | <li><a href="https://2012.igem.org/Team:Trieste/parts/5">BBa_K875005</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/4">BBa_K875004 - OmpA scFv</a></li> |
| - | <li><a href="https://2012.igem.org/Team:Trieste/parts/6">BBa_K875006</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/5">BBa_K875005 - OmpA SIP</a></li> |
| - | <li><a href="https://2012.igem.org/Team:Trieste/parts/7">BBa_K875007</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/6">BBa_K875006 - PelB scFv</a></li> |
| - | <li><a href="https://2012.igem.org/Team:Trieste/parts/8">BBa_K875008</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/7">BBa_K875007 - PelB SIP </a></li> |
| - | <li><a href="https://2012.igem.org/Team:Trieste/parts/9">BBa_K875009</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/8">BBa_K875008 - Tse2 Toxin</a></li> |
| - | <li><a href="https://2012.igem.org/Team:Trieste/parts/10">BBa_K875020</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/9">BBa_K875009 - LL 37</a></li> |
| - | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/10">BBa_K875020 - Glucosidase</a></li> | |
| + | </strong> | ||
</ul> | </ul> | ||
<img src="https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg" alt="Team iGEM 2012" id="igem_team" /> | <img src="https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg" alt="Team iGEM 2012" id="igem_team" /> | ||
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Follow us also: | Follow us also: | ||
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| - | <a href="https://twitter.com/ | + | <a href="https://twitter.com/iGEMTrieste" target="_blank"><img src="https://static.igem.org/mediawiki/2012/2/21/Ico_twitter.png" alt="twitter" class="tw" /></a> |
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 12:03, 26 October 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Parts Overview
More
List of the Submitted Parts
Click on the code of each part to have more information about the Biobrick, its construction, the results obtained and the relative link to the Registry.
| Name | Type | Description | ||
| BBa_K875001 | Regulatory | T5 Cumate Operator | ||
| BBa_K875002 | Regulatory | T5 Lac Operator | ||
| BBa_K875003 | Composit | CymR | ||
| BBa_K875004 | Composit | T5LacO-LPP-OmpA-scFv | ||
| BBa_K875005 | Composit | T5LacO-LPP-OmpA-SIP | ||
| BBa_K875006 | Composit | T5LacO-LPP-PelB-scFv | ||
| BBa_K875007 | Composit | T5 LacO-LPP-PelB-SIP | ||
| BBa_K875008 | Coding | IPTG inducible Tse2 toxin | ||
| BBa_K875009 | Coding | LL 37 - Cathelicidin | ||
| BBa_K875020 | Composit | β-Glucosidase |
Primers
| Name | Seq 5'--> 3' | PCR Notes |
|---|---|---|
| Vr | ATTACCGCCTTTGAGTGAGC | 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞ |
| Vf2 | TGCCACCTGACGTCTAAGAA | 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞ |
| GlmSTn7Fw | GCATGTGGAAGAGGTGATTG | 95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| GlmSTn7Rev | ACTTTATTGTTCGCCCACA | 95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| B0015XhoRev | ACTCGAGATTACTAGTTATAAACGCAGAAAGGCCCACCC | 95° 5' | 30x (93° 30'' | 58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| B0015EcoFw | CGAATTCATCAGATCTCCAGGCATCAAATAAAACGAAAGG | 95° 5' | 30x (93° 30'' |58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| T5EcoFw | CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT | 95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| T5XhoRev | TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA | 95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta1/2FW | ATACTACGACGGTAAATGGT | 95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta1/2REV | TACATCAGTATCGGTAGCAT | 95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta7/8FW | GACCAAGCGATAACCGGATG | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta7/8REV | GTGAGATGATGGCCACGATT | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta9/10FW | GCGAGGTAACCTCGAACATG | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta9/10REV | CGGCGTATCGATAATTCACG | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
