Team:Trieste/parts
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<div id="box_main"> <!-- start box_main --> | <div id="box_main"> <!-- start box_main --> | ||
<div class="box_contenuti"> | <div class="box_contenuti"> | ||
- | <h2>List of the | + | <h2>List of the Submitted Parts </h2> |
- | <h3>Click on the code of each part to have more information about the Biobrick | + | <h3>Click on the code of each part to have more information about the Biobrick, its construction, the results obtained and the relative link to the Registry.</h3> |
</br> | </br> | ||
</br> | </br> | ||
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<td><strong>Type</strong></td> | <td><strong>Type</strong></td> | ||
<td></td> | <td></td> | ||
- | <td><strong> | + | <td><strong>Description</strong></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2012.igem.org/Team:Trieste/parts/1">BBa_K875001</a></td> | <td><a href="https://2012.igem.org/Team:Trieste/parts/1">BBa_K875001</a></td> | ||
+ | <td></td> | ||
<td>Regulatory</td> | <td>Regulatory</td> | ||
+ | <td></td> | ||
<td>T5 Cumate Operator</td> | <td>T5 Cumate Operator</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><a href="https://2012.igem.org/Team:Trieste/parts/2">BBa_K875002</a></td> | + | <td><a href="https://2012.igem.org/Team:Trieste/parts/2">BBa_K875002</a></td> |
+ | <td></td> | ||
<td>Regulatory</td> | <td>Regulatory</td> | ||
- | <td>T5 Lac | + | <td></td> |
+ | <td>T5 Lac Operator</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2012.igem.org/Team:Trieste/parts/3">BBa_K875003</a></td> | <td><a href="https://2012.igem.org/Team:Trieste/parts/3">BBa_K875003</a></td> | ||
+ | <td></td> | ||
<td>Composit</td> | <td>Composit</td> | ||
+ | <td></td> | ||
<td>CymR</td> | <td>CymR</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2012.igem.org/Team:Trieste/parts/4">BBa_K875004</a></td> | <td><a href="https://2012.igem.org/Team:Trieste/parts/4">BBa_K875004</a></td> | ||
+ | <td></td> | ||
<td>Composit</td> | <td>Composit</td> | ||
+ | <td></td> | ||
<td>T5LacO-LPP-OmpA-scFv</td> | <td>T5LacO-LPP-OmpA-scFv</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2012.igem.org/Team:Trieste/parts/5">BBa_K875005</a></td> | <td><a href="https://2012.igem.org/Team:Trieste/parts/5">BBa_K875005</a></td> | ||
+ | <td></td> | ||
<td>Composit</td> | <td>Composit</td> | ||
+ | <td></td> | ||
<td>T5LacO-LPP-OmpA-SIP</td> | <td>T5LacO-LPP-OmpA-SIP</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2012.igem.org/Team:Trieste/parts/6">BBa_K875006</a></td> | <td><a href="https://2012.igem.org/Team:Trieste/parts/6">BBa_K875006</a></td> | ||
+ | <td></td> | ||
<td>Composit</td> | <td>Composit</td> | ||
+ | <td></td> | ||
<td>T5LacO-LPP-PelB-scFv</td> | <td>T5LacO-LPP-PelB-scFv</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2012.igem.org/Team:Trieste/parts/7">BBa_K875007</a></td> | <td><a href="https://2012.igem.org/Team:Trieste/parts/7">BBa_K875007</a></td> | ||
+ | <td></td> | ||
<td>Composit</td> | <td>Composit</td> | ||
+ | <td></td> | ||
<td>T5 LacO-LPP-PelB-SIP</td> | <td>T5 LacO-LPP-PelB-SIP</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2012.igem.org/Team:Trieste/parts/8">BBa_K875008</a></td> | <td><a href="https://2012.igem.org/Team:Trieste/parts/8">BBa_K875008</a></td> | ||
+ | <td></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
+ | <td></td> | ||
<td>IPTG inducible Tse2 toxin</td> | <td>IPTG inducible Tse2 toxin</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2012.igem.org/Team:Trieste/parts/9">BBa_K875009</a></td> | <td><a href="https://2012.igem.org/Team:Trieste/parts/9">BBa_K875009</a></td> | ||
+ | <td></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
+ | <td></td> | ||
<td>LL 37 - Cathelicidin</td> | <td>LL 37 - Cathelicidin</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2012.igem.org/Team:Trieste/parts/10">BBa_K875020</a></td> | <td><a href="https://2012.igem.org/Team:Trieste/parts/10">BBa_K875020</a></td> | ||
+ | <td></td> | ||
<td>Composit</td> | <td>Composit</td> | ||
+ | <td></td> | ||
<td>β-Glucosidase</td> | <td>β-Glucosidase</td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
- | </center> | + | </center> |
+ | <br/> | ||
+ | <h2>Primers </h2> | ||
+ | <table> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th> Name</th> | ||
+ | <th> Seq 5'--> 3'</th> | ||
+ | <th> PCR Notes</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | |||
+ | <tbody> | ||
+ | <tr><td>Vr</td> <td>ATTACCGCCTTTGAGTGAGC</td> <td>95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞</td></tr> | ||
+ | <tr><td>Vf2</td> <td>TGCCACCTGACGTCTAAGAA</td> <td> 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞</td></tr> | ||
+ | <tr><td>GlmSTn7Fw</td> <td>GCATGTGGAAGAGGTGATTG</td> <td>95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr> | ||
+ | <tr><td>GlmSTn7Rev</td> <td>ACTTTATTGTTCGCCCACA</td> <td>95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr> | ||
+ | <tr><td>B0015XhoRev</td> <td>ACTCGAGATTACTAGTTATAAACGCAGAAAGGCCCACCC</td> <td>95° 5' | 30x (93° 30'' | 58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr> | ||
+ | <tr><td>B0015EcoFw</td> <td>CGAATTCATCAGATCTCCAGGCATCAAATAAAACGAAAGG</td> <td>95° 5' | 30x (93° 30'' |58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr> | ||
+ | <tr><td>T5EcoFw</td> <td>CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT</td> <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
+ | <tr><td>T5XhoRev</td> <td>TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA</td> <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
+ | <tr><td>Muta1/2FW</td> <td>ATACTACGACGGTAAATGGT</td> <td>95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
+ | <tr><td>Muta1/2REV</td> <td>TACATCAGTATCGGTAGCAT</td> <td>95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
+ | <tr><td>Muta7/8FW</td> <td>GACCAAGCGATAACCGGATG</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
+ | <tr><td>Muta7/8REV</td> <td>GTGAGATGATGGCCACGATT</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
+ | <tr><td>Muta9/10FW</td> <td>GCGAGGTAACCTCGAACATG</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
+ | <tr><td>Muta9/10REV</td> <td>CGGCGTATCGATAATTCACG</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
<!-- ******************** CONTENUTO QUI ****************** --> | <!-- ******************** CONTENUTO QUI ****************** --> | ||
</div> | </div> | ||
</div> <!-- end box_main --> | </div> <!-- end box_main --> | ||
<div id="box_right"> <!-- start box_right --> | <div id="box_right"> <!-- start box_right --> | ||
- | <ul id="sub_menu"> | + | <ul id="sub_menu"><strong> |
- | <li><a href="https://2012.igem.org/Team:Trieste/parts/1">BBa_K875001</a></li> | + | <li class="select"><a href="https://2012.igem.org/Team:Trieste/parts">Overwiev</a></li> |
- | <li><a href="https://2012.igem.org/Team:Trieste/parts/2">BBa_K875002</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/1">BBa_K875001 - Cumate Op</a></li> |
- | <li><a href="https://2012.igem.org/Team:Trieste/parts/3">BBa_K875003</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/2">BBa_K875002 - Lac OP</a></li> |
- | <li><a href="https://2012.igem.org/Team:Trieste/parts/4">BBa_K875004</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/3">BBa_K875003 - CymR</a></li> |
- | <li><a href="https://2012.igem.org/Team:Trieste/parts/5">BBa_K875005</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/4">BBa_K875004 - OmpA scFv</a></li> |
- | <li><a href="https://2012.igem.org/Team:Trieste/parts/6">BBa_K875006</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/5">BBa_K875005 - OmpA SIP</a></li> |
- | <li><a href="https://2012.igem.org/Team:Trieste/parts/7">BBa_K875007</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/6">BBa_K875006 - PelB scFv</a></li> |
- | <li><a href="https://2012.igem.org/Team:Trieste/parts/8">BBa_K875008</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/7">BBa_K875007 - PelB SIP </a></li> |
- | <li><a href="https://2012.igem.org/Team:Trieste/parts/9">BBa_K875009</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/8">BBa_K875008 - Tse2 Toxin</a></li> |
- | <li><a href="https://2012.igem.org/Team:Trieste/parts/10">BBa_K875020</a></li> | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/9">BBa_K875009 - LL 37</a></li> |
- | + | <li><a href="https://2012.igem.org/Team:Trieste/parts/10">BBa_K875020 - Glucosidase</a></li> | |
+ | </strong> | ||
</ul> | </ul> | ||
<img src="https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg" alt="Team iGEM 2012" id="igem_team" /> | <img src="https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg" alt="Team iGEM 2012" id="igem_team" /> | ||
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Follow us also: | Follow us also: | ||
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- | <a href="https://twitter.com/ | + | <a href="https://twitter.com/iGEMTrieste" target="_blank"><img src="https://static.igem.org/mediawiki/2012/2/21/Ico_twitter.png" alt="twitter" class="tw" /></a> |
</div> | </div> | ||
</div> | </div> |
Latest revision as of 12:03, 26 October 2012
Parts Overview
More
List of the Submitted Parts
Click on the code of each part to have more information about the Biobrick, its construction, the results obtained and the relative link to the Registry.
Name | Type | Description | ||
BBa_K875001 | Regulatory | T5 Cumate Operator | ||
BBa_K875002 | Regulatory | T5 Lac Operator | ||
BBa_K875003 | Composit | CymR | ||
BBa_K875004 | Composit | T5LacO-LPP-OmpA-scFv | ||
BBa_K875005 | Composit | T5LacO-LPP-OmpA-SIP | ||
BBa_K875006 | Composit | T5LacO-LPP-PelB-scFv | ||
BBa_K875007 | Composit | T5 LacO-LPP-PelB-SIP | ||
BBa_K875008 | Coding | IPTG inducible Tse2 toxin | ||
BBa_K875009 | Coding | LL 37 - Cathelicidin | ||
BBa_K875020 | Composit | β-Glucosidase |
Primers
Name | Seq 5'--> 3' | PCR Notes |
---|---|---|
Vr | ATTACCGCCTTTGAGTGAGC | 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞ |
Vf2 | TGCCACCTGACGTCTAAGAA | 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞ |
GlmSTn7Fw | GCATGTGGAAGAGGTGATTG | 95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
GlmSTn7Rev | ACTTTATTGTTCGCCCACA | 95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
B0015XhoRev | ACTCGAGATTACTAGTTATAAACGCAGAAAGGCCCACCC | 95° 5' | 30x (93° 30'' | 58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
B0015EcoFw | CGAATTCATCAGATCTCCAGGCATCAAATAAAACGAAAGG | 95° 5' | 30x (93° 30'' |58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
T5EcoFw | CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT | 95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
T5XhoRev | TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA | 95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
Muta1/2FW | ATACTACGACGGTAAATGGT | 95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
Muta1/2REV | TACATCAGTATCGGTAGCAT | 95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
Muta7/8FW | GACCAAGCGATAACCGGATG | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
Muta7/8REV | GTGAGATGATGGCCACGATT | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
Muta9/10FW | GCGAGGTAACCTCGAACATG | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
Muta9/10REV | CGGCGTATCGATAATTCACG | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
![Team iGEM 2012](https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg)
![iGEM 2012](https://static.igem.org/mediawiki/2012/f/f0/Trieste-ombra_left.png)
![iGEM 2012](https://static.igem.org/mediawiki/2012/c/cb/Ombra_right.png)
![iGEM 2012](https://static.igem.org/mediawiki/2012/3/3a/Trieste-ombra_vert_left.png)
![iGEM 2012](https://static.igem.org/mediawiki/2012/3/3a/Trieste-ombra_vert_left.png)
![iGEM 2012](https://static.igem.org/mediawiki/2012/3/3a/Trieste-ombra_vert_left.png)
![iGEM 2012](https://static.igem.org/mediawiki/2012/b/b3/Trieste-ombra_vert_right.png)