Team:XMU-China

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   <p>  XMU's 2012 iGEM team has constructed a fluorescent digital display device with synthetic logic gates, which is able to respond to signals by displaying and switching numbers. We put GFP equipped with degradation tags in downstream to illuminate our numbers and change them quickly as well. Considering our engineering background, we accordingly employ cell immobilization to build our device. Engineering bacteria have been embedded in intra-hallow calcium alginate microcapsules and in PDMDAAC-NaCS microcapsules, respectively. In addition, 3D CAD design is performed for a perfect device&#8230;
   <p>  XMU's 2012 iGEM team has constructed a fluorescent digital display device with synthetic logic gates, which is able to respond to signals by displaying and switching numbers. We put GFP equipped with degradation tags in downstream to illuminate our numbers and change them quickly as well. Considering our engineering background, we accordingly employ cell immobilization to build our device. Engineering bacteria have been embedded in intra-hallow calcium alginate microcapsules and in PDMDAAC-NaCS microcapsules, respectively. In addition, 3D CAD design is performed for a perfect device&#8230;
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   <hr><a href="https://2012.igem.org/Team:XMU-China/abstract">[see more]</a></div>
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  <a href="https://2012.igem.org/Template:Team:XMU-China/abstract">[more]</a></div>
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     <div class="tabcon dis">Sunday, August 26th<br>
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     <div class="tabcon dis"><p style="font-size:16px"><strong>Asian iGEM Jamboree, 5-7 October, HKUST</strong></p> <br>
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Digital Display:
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XMU-China has won a GOLD medal and become one of the 17 out of 53 teams advancing to World Championship . We are looking forward to present our project to the whole world at MIT. You are warmly welcomed to attend.<br><br>
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Ligated 18AI and RETPcPb and put the ligation product in 16ºC overnight for the transformation in the next day.<hr>
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<b>Venue: Room 32-123</b><br><br>
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<b>Date: 11:00 AM, Nov 3rd, Saturday</b><br><br>
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Time Delay:
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Goal: To do the ligation of pBAD-RBS0.6 and pBAD-RBS0.07.
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<a href="https://2012.igem.org/Team:XMU-China/wetlabjournal">[see more]</a>
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Isolate plasmids and ran on a gel. The result for analysis was correct. Cause the bands of pBAD hardly to be seem, we increased the volume of pBAD when ligation. <hr>
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Fluorescence Test:
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We changed the abrabinose concentration from high levels to lower ones according to last fluorescence measurements of PBADRLT. Besides, we took some kinetic curves in all concentration levels we had taken before for comparison. The analysis of these data will help us improve our efficiency in the coming fluorescence measurements<hr>
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Design:
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We did some card design and drew some drafts.<hr>
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<a href="https://2012.igem.org/Template:Team:XMU-China/wetlabjournal">[more]</a>
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     <!--device description-->
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         DEVICE DESCRIPTION</p>
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         DEVICE DEMONSTRATION</p>
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       <p style=" font-size:28px; color:#004f7c">E.LUMOLI DISPLAYER</p>
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       <span style=" font-size:28px; color:#004f7c; line-height:24px">E.LUMOLI DISPLAYER</span></p>
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      <p style=" font-size:18px;"><img src="https://static.igem.org/mediawiki/2012/a/a4/Device.png
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      <div id="description_content">By adding different inducers, cells in tubes can respond to different signals by display and switching numbers.
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      <p style=" font-size:28px; color:#004f7c">&nbsp;</p>
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     <!--device description end-->
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<td id="title" width="300">Immobilization</td>
<td id="title" width="300">Immobilization</td>
<td id="title" width="300">Time delay</td></tr>
<td id="title" width="300">Time delay</td></tr>
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<tr><td id="rb">Synthetic circuits with genetic logic gates, which compute extracellular and intracellular signals and elicit response differently.<a id="more" href="https://2012.igem.org/Template:Team:XMU-China/digitaldisplay">[more]</a></td>
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<tr><td id="rb">Synthetic circuits with genetic logic gates, which compute extracellular and intracellular signals and elicit response differently.<a id="more" href="https://2012.igem.org/Team:XMU-China/digitaldisplay">[see more]</a></td>
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<td id="rb">Immobilization of Fluorescent <I>E.coli</I> cells .Design A Bioreactor for Numeric Display<a id="more" href="https://2012.igem.org/Template:Team:XMU-China/cellimmobilization">[more]</a></td>
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<td id="rb">Immobilization of fluorescent <I>E.coli</I> cells. Design a bioreactor for numeric display.<a id="more" href="https://2012.igem.org/Team:XMU-China/cellimmobilization">[see more]</a></td>
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<td><p>Testing the delay of time for GFP expression when we use different strength of RBS<a id="more" href="https://2012.igem.org/Template:Team:XMU-China/timedelay">[more]</a></p></td></tr>
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<td><p>Testing the delay of time for GFP expression when we use different strength of RBS.<a id="more" href="https://2012.igem.org/Team:XMU-China/timedelay">[see more]</a></p></td></tr>
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Latest revision as of 03:05, 27 October 2012

XMU



homepage

ABSTRACT
PROGRESS

XMU's 2012 iGEM team has constructed a fluorescent digital display device with synthetic logic gates, which is able to respond to signals by displaying and switching numbers. We put GFP equipped with degradation tags in downstream to illuminate our numbers and change them quickly as well. Considering our engineering background, we accordingly employ cell immobilization to build our device. Engineering bacteria have been embedded in intra-hallow calcium alginate microcapsules and in PDMDAAC-NaCS microcapsules, respectively. In addition, 3D CAD design is performed for a perfect device…

[see more]

Asian iGEM Jamboree, 5-7 October, HKUST


XMU-China has won a GOLD medal and become one of the 17 out of 53 teams advancing to World Championship . We are looking forward to present our project to the whole world at MIT. You are warmly welcomed to attend.

Venue: Room 32-123

Date: 11:00 AM, Nov 3rd, Saturday

[see more]

DEVICE DEMONSTRATION

E.LUMOLI DISPLAYER


By adding different inducers, cells in tubes can respond to different signals by display and switching numbers.

 

Digital Display Immobilization Time delay
Synthetic circuits with genetic logic gates, which compute extracellular and intracellular signals and elicit response differently.[see more] Immobilization of fluorescent E.coli cells. Design a bioreactor for numeric display.[see more]

Testing the delay of time for GFP expression when we use different strength of RBS.[see more]




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