Team:BAU-Indonesia

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<li><a href='http://ipb.ac.id/' target="_blank" title='IPB'> Bogor Agricultural University </a></li>
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<li><a href='http://fmipa.ipb.ac.id/' target="_blank" title='FMIPA'> Faculty of Mathematics and Natural Sciences </a></li>
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<li><a href='http://biology-ipb.org/' target="_blank" title='Biology'> Department of Biology </a></li>
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<li><a href='http://cs.ipb.ac.id/' target="_blank" title='CS'> Department of Computer Science</a> </li>
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      <h3>Daftar isi</h3>
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         <div id="isinya">isi konten di sini bisa juga dengan java script atau yang lainnya</div>
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    </li>
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    <li>
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      <h3>F1</h3>
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        <div id="isinya">pokonya apa saja isi di sini terserah mau di isi apa</div>
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      <h3>Free Dfloaf</h3>
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        <div id="isinya">isi apa saja terserah sobat</div>
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      <h3>Free Dosfs</h3>
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        <div id="isinya">isi konten  di sini bisa dengan gambar atau yang lainnya</div>
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<h2>Welcome To Bogor Agricultural University Project</h2>
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<h2><b>All About iGEM </h2><h3>International Genetically Engineered Machine</b></h3>
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<p style="text-align:justify"><b>iGEM is one of the most prestigious of international genetic
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Nam sed massa nunc. Duis malesuada elit a orci auctor imperdiet ut vitae mauris. Proin ultrices est non dolor mollis condimentum at vel orci. Sed tristique ultricies orci sit amet placerat. Etiam tincidunt vestibulum arcu, in malesuada nibh mollis a. Aliquam erat volutpat. Sed ac</p>
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  competition for students of optimizing innovation and creativity for a
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<br />
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  better life. This time, Indonesia is participating by the team which
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  is come from Bogor Agricultural University. 10 undergraduate students
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Sed tristique ultricies orci sit amet placerat. Etiam tincidunt vestibulum arcu, in malesuada nibh mollis a. Aliquam erat volutpat. Sed ac posuere odio. Etiam eu purus mi. Nunc dictum metus non nunc ultrices malesuada. Quisque dictum eros id metus lobortis hendrerit. Donec mollis venenatis pellentesque. Praesent massa enim, varius nec commodo eget, varius mollis augue. Mauris sodales vestibulum est, </p>
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  and 2 faculty advisor (link to profile team) have a passion in
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<div class="read"><a href="#">read more &raquo;</a></div>
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  environmental molecular science. BAU-Indonesia is a team that has a purpose to
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  degrade plastic on the ground such as : PET, PE, PP and PVC. Indonesia
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  is still active in the use of these kinds of plastic but the
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  degradation itself is still not effective and efficient. The existence
 +
  of plastic waste in the river will spread to the sea or ocean. If it's
 +
  not being eliminated or degraded, Earth would be fulfilled with a pile
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  of plastic either at terrestrial or water that will disturb the living
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  system. It can kill living organism which is started from microbe,
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  plant, animal, and human. Based on those reasons, BAU-Indonesia team will make
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  the solution to get the plastic degradation enzyme from bacteria,
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    especially the enzyme to degrade PE and PET. </b></p>
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<h2>Welcome To Bogor Agricultural University Project</h2>
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<h2><b>Project Background</b></h2>
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<div class="text"><h3><b>Introduction</b></h3>
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<p style="text-align:justify"><b>Polyethylene is a synthetic polymer that made of long chain monomers of ethylene. It is a thermoplastic commodity mostly used for packaging. About 140 million tonnes of synthetic polymers are produced worldwide annually with their utility escalating at a rate of 12% per annum (Shimao 2001). The most widely used plastics used in packaging are polyethylene (LDPE, MDPE, HDPE and LLDPE), polypropylene (PP), polystyrene (PS), polyvinyl chloride (PVC), polyurethane (PUR), poly(ethylene terephthalate) (PET), poly (butylene terephthalate) (PBT). Polyethylene are resistant against microbial attack, since during their short time of presence in nature evolution could not design new enzyme structures capable of degrading synthetic polymers (Mueller 2006).  In order to manage the utility of these polymers in the nature, there are two ways: one is to exploit the microorganisms in degrading polyethylene and the other is to develop artificial polymers susceptible to biodegradation. Subsequently, to gain large-scale acceptance these man-made biodegradable polyethylene should retain all the essential properties of utility by the consumer and when discarded in the environment should demonstrate their degradability more rapidly than the conventional ones (El-Shafei et al. 1998).
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<br> Nanda et al. (2010) have reported natural and synthetic PE degradation activity of Pseudomonas sp. that isolated from sewage sludge and household garbage dump. The degradation activity from each Pseudomonas sp. isolates were different between natural and synthetic PE 31,4%-46,2% and 29,1% - 16,3% respectively in loss weight. Some kind of enzymes had a PE degradation activity reported by Guebitz  and Cavaco-Paulo (2008) cutinase. Cutinase has recently received much attention because of its potential application for surface modification and degradation of aliphatic and aromatic polyesters, especially polyethylene terephthalate (PET), which is a synthetic aromatic polyester composed of terephthalic acid (TPA) and ethylene glycol) ; however, the number of cutinases, which have been studied regarding PET modification, is still limited, and this limitation may result in the delay of the research toward the practical use of cutinases. Bogor Agricultural University (IPB) iGEM team project is making a synthetic bacteria that can degrade plastic ecspecially PE/PET with a novel degrading enzymes.<b>
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<h3><b>References</b></h3>
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<p style="text-align:justify">
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<b>El-Shafei HA, El-Nasser NHA, Kansoh AL, Ali AM .1998. Biodegradation of disposable polyethylene by fungi and Streptomyces species. Polym. Degrad. Stab. 62: 361 - 365.<br><br>
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Guebitz GM, Cavaco-Paulo A. 2008. Enzymes go big: surface hydrolysis and functionalization of synthetic polymers. Trends Biotechnol. 26:32–38.<br><br>
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 +
Shimao, M (2001). Biodegradation of plastics. Curr. Opin. Biotechnol. 12: 242 - 247.<br><br>
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Mueller RJ. 2006. Biological degradation of synthetic polyesters—enzymes as  potential catalysts for polyester recycling. Proc Biochem 41:2124–8.<br><br>
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Nanda S, Snigdha S, Abraham J.2010. Studies on the biodegradation of natural and synthetic polyethylene by Pseudomonas spp. J. Appl. Sci. Environ. Manage. Vol. 14 (2) 57 – 60.<br>
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<h2>Latest News</h2>
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<h2><b>Abstract</b></h2><p>==Plastic Terminator==</p>
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<p style="text-align:justify"><b>Indonesia is known as the 4th highest population densities around the world. Nowadays, 1.5 million tons/year from human activity which is used in the world is PET. PET is a thermoplastic polymer resin, not easily degraded naturally. Based on this background, the BAU-Indonesia team designs a plasmid which is contains of encoding cutinase degrading enzymes of producing PET. <br>The early stage of this project was done by the preservation of plastic waste bacteria from landfills at Galuga. The bacteria were cultured in liquid media which is contained yeast extract powder and PET enrichment. The result of this preservation will be followed by the isolation of DNA and PCR with Cutinase F primer'ACGCGCCGGGCGTCACCGAGCA'3 and R 5'ACGCGTCGTGCCGTCAGGGCCA'3. Cutinase gen that were amplified will be inserted to plasmid pSB1C3. The recombinant plasmid which contained the cutinase gene will be introduced into E. coli. Finally it will be used as PET biodegradator product. </b></p>
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<h2>Testimonials</h2>
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<h2><b>Wise Words</b></h2>
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<p class="italic_style">&quot;Praesent sagittis feugiat orci, eu suscipit justo dapibus nec. Duis at lacinia metus. Morbi interdum, justo ac blandit aliquet, nunc odio laoreet justo, vel accumsan libero&quot;</p>
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<p class="italic_style" style="text-align:justify">&quot;We don't afraid of failure
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We dare to take risks
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        to achieve success
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We are extraordinary people
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        with a brave fight
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        to try new things
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For us, risk doesn't mean anything...
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For us, failure is not a failure
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Success for us is created because we got a lot of failure.<br><br>
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With this failure,
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we find identity
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We believe the Lord bless the way that we live in this moment,
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because we dare to fail !<br><br>
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We just need to find a way
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the most appropriate way to achieve success<br><br>
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We get much rejection
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because that is what makes
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us be more motivated<br><br>
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All failures are
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good lesson for us<br><br>
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We still believe
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There is a chance
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    to learn ... to search ...
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and serving the best&quot;</p>
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<div class="read"><a>Odop for Us</a></div>
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Latest revision as of 22:40, 26 September 2012


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All About iGEM

International Genetically Engineered Machine

iGEM is one of the most prestigious of international genetic competition for students of optimizing innovation and creativity for a better life. This time, Indonesia is participating by the team which is come from Bogor Agricultural University. 10 undergraduate students and 2 faculty advisor (link to profile team) have a passion in environmental molecular science. BAU-Indonesia is a team that has a purpose to degrade plastic on the ground such as : PET, PE, PP and PVC. Indonesia is still active in the use of these kinds of plastic but the degradation itself is still not effective and efficient. The existence of plastic waste in the river will spread to the sea or ocean. If it's not being eliminated or degraded, Earth would be fulfilled with a pile of plastic either at terrestrial or water that will disturb the living system. It can kill living organism which is started from microbe, plant, animal, and human. Based on those reasons, BAU-Indonesia team will make the solution to get the plastic degradation enzyme from bacteria, especially the enzyme to degrade PE and PET.

Project Background

Introduction

Polyethylene is a synthetic polymer that made of long chain monomers of ethylene. It is a thermoplastic commodity mostly used for packaging. About 140 million tonnes of synthetic polymers are produced worldwide annually with their utility escalating at a rate of 12% per annum (Shimao 2001). The most widely used plastics used in packaging are polyethylene (LDPE, MDPE, HDPE and LLDPE), polypropylene (PP), polystyrene (PS), polyvinyl chloride (PVC), polyurethane (PUR), poly(ethylene terephthalate) (PET), poly (butylene terephthalate) (PBT). Polyethylene are resistant against microbial attack, since during their short time of presence in nature evolution could not design new enzyme structures capable of degrading synthetic polymers (Mueller 2006). In order to manage the utility of these polymers in the nature, there are two ways: one is to exploit the microorganisms in degrading polyethylene and the other is to develop artificial polymers susceptible to biodegradation. Subsequently, to gain large-scale acceptance these man-made biodegradable polyethylene should retain all the essential properties of utility by the consumer and when discarded in the environment should demonstrate their degradability more rapidly than the conventional ones (El-Shafei et al. 1998).
Nanda et al. (2010) have reported natural and synthetic PE degradation activity of Pseudomonas sp. that isolated from sewage sludge and household garbage dump. The degradation activity from each Pseudomonas sp. isolates were different between natural and synthetic PE 31,4%-46,2% and 29,1% - 16,3% respectively in loss weight. Some kind of enzymes had a PE degradation activity reported by Guebitz and Cavaco-Paulo (2008) cutinase. Cutinase has recently received much attention because of its potential application for surface modification and degradation of aliphatic and aromatic polyesters, especially polyethylene terephthalate (PET), which is a synthetic aromatic polyester composed of terephthalic acid (TPA) and ethylene glycol) ; however, the number of cutinases, which have been studied regarding PET modification, is still limited, and this limitation may result in the delay of the research toward the practical use of cutinases. Bogor Agricultural University (IPB) iGEM team project is making a synthetic bacteria that can degrade plastic ecspecially PE/PET with a novel degrading enzymes.


References

El-Shafei HA, El-Nasser NHA, Kansoh AL, Ali AM .1998. Biodegradation of disposable polyethylene by fungi and Streptomyces species. Polym. Degrad. Stab. 62: 361 - 365.

Guebitz GM, Cavaco-Paulo A. 2008. Enzymes go big: surface hydrolysis and functionalization of synthetic polymers. Trends Biotechnol. 26:32–38.

Shimao, M (2001). Biodegradation of plastics. Curr. Opin. Biotechnol. 12: 242 - 247.

Mueller RJ. 2006. Biological degradation of synthetic polyesters—enzymes as potential catalysts for polyester recycling. Proc Biochem 41:2124–8.

Nanda S, Snigdha S, Abraham J.2010. Studies on the biodegradation of natural and synthetic polyethylene by Pseudomonas spp. J. Appl. Sci. Environ. Manage. Vol. 14 (2) 57 – 60.

Abstract

==Plastic Terminator==

Indonesia is known as the 4th highest population densities around the world. Nowadays, 1.5 million tons/year from human activity which is used in the world is PET. PET is a thermoplastic polymer resin, not easily degraded naturally. Based on this background, the BAU-Indonesia team designs a plasmid which is contains of encoding cutinase degrading enzymes of producing PET.
The early stage of this project was done by the preservation of plastic waste bacteria from landfills at Galuga. The bacteria were cultured in liquid media which is contained yeast extract powder and PET enrichment. The result of this preservation will be followed by the isolation of DNA and PCR with Cutinase F primer'ACGCGCCGGGCGTCACCGAGCA'3 and R 5'ACGCGTCGTGCCGTCAGGGCCA'3. Cutinase gen that were amplified will be inserted to plasmid pSB1C3. The recombinant plasmid which contained the cutinase gene will be introduced into E. coli. Finally it will be used as PET biodegradator product.

Wise Words

"We don't afraid of failure We dare to take risks to achieve success We are extraordinary people with a brave fight to try new things For us, risk doesn't mean anything... For us, failure is not a failure Success for us is created because we got a lot of failure.

With this failure, we find identity We believe the Lord bless the way that we live in this moment, because we dare to fail !

We just need to find a way the most appropriate way to achieve success

We get much rejection because that is what makes us be more motivated

All failures are good lesson for us

We still believe There is a chance to learn ... to search ... and serving the best"