Team:Evry/OrthogonalSyst

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<h1>Orthogonal hormonal system</h1>
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<p>Our orthogonal hormonal system is based on the use of auxin, a plant hormone. To implement this system, we used two different types of device corresponding to the production and the reception of auxin in the tadpole.</p>
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<h2>Auxin production devices</h2>
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<p>We envisaged 3 ways of design our auxin production device in tadpoles. The devices 1 and 2 have been designed to be expressed in tadpole's embryos, while device 3 have been designed to be expressed in E.coli. In this last case, the aim is that the tadpoles eat bacteria expressing device 3.</p>
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<img src="https://static.igem.org/mediawiki/2012/c/c2/3devices.jpg" width="600px" alt="3 devices for production" />
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<ul>
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<li> <b>Auxin production device 1 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812121">BBa_K812021</a>, coding for IaaM, and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812120">BBa_K812120</a>, coding for IaaH for auxin generator for the use in tadpole's embryos.
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<li> <b>Auxin production device 2 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812014">BBa_K812014</a>. It is ment for the co-expression of IaaH and IaaM genes in the same cell in tadpole.
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<li> <b>Auxin production device 3 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515100">BBa_K515100</a>, coding for IaaM and IaaH for auxin generator in <i>E.coli</i>.
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<img src="https://static.igem.org/mediawiki/2012/b/bd/ProductiondeviceCompress.jpg" width="900px" alt="Production devices" />
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<h3>Pathway</h3>
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<img src="https://static.igem.org/mediawiki/2012/4/4b/Pathway2.jpg" width="600px" alt="Auxin pathway" />
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<h2>Auxin reception device</h2>
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<p>We envisaged 2 ways of design our auxin production device in tadpoles.</p>
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<ul>
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<li> <b>Auxin reception device 1 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812010">BBa_K812010</a> coding for GFP-AID, and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812012">BBa_K812012</a> coding for OsTir1.
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<li> <b>Auxin reception device 2 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812013">BBa_K812013</a> coding for GFP-AID and OsTir1 in the same cell.
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</ul></br>
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<img src="https://static.igem.org/mediawiki/2012/1/1e/Recptiondevice.jpg" width="930px" alt="devices for reception" />
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<h3>Pathway</h3>
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<img src="https://static.igem.org/mediawiki/2012/c/ce/AIDsyst.jpg" width="900px" alt="Production devices" />
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<div id="citation_box">
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<p id="references">References:</p>
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<li><i>Inducible control of tissue-specific transgene expression in Xenopus tropicalis transgenic lines.</i>, Chae J., Zimmerman L.B., Grainger R.M., Mechanisms of development 117:1-2, 2002</li>
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<li><i>Xenopus: a prince among models for pronephric kidney development.</i>, Jones E., JASN 16:2, 2005</li>
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<li><i>An auxin-based degron system for the rapid depletion of proteins in nonplant cells</i>, Nishimura K., Fukagawa T., Takisawa H., Kakimoto T., Kanemaki M., Nature Methods 6:12, 2009</li>
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Latest revision as of 20:51, 23 September 2012

moved to https://2012.igem.org/Team:Evry/AIDSystem

Retrieved from "http://2012.igem.org/Team:Evry/OrthogonalSyst"