|
|
(9 intermediate revisions not shown) |
Line 3: |
Line 3: |
| = Results = | | = Results = |
| | | |
- | == The Constructor ==
| + | An overview of the results can be found [[Wageningen_UR/FinalOverview|here]] |
- | | + | |
- | More efficient cloning, publication, importance of computational work in SynBio
| + | |
- | | + | |
- | {| class="wikitable"
| + | |
- | ! iGEM 2011 team
| + | |
- | ! iGEM project title
| + | |
- | ! # of TU's in the designed circuit
| + | |
- | ! # of used Biobrick Parts By team
| + | |
- | ! Recommended by the Constructor
| + | |
- | ! Processing time (min)
| + | |
- | | + | |
- | |-
| + | |
- | | KAIST-Korea<sup>1</sup> || E. casso ||style="text-align:center;"| 2 ||style="text-align:center;"| 11 ||style="text-align:center;"| 7 ||style="text-align:center;"| < 1 min
| + | |
- | |-
| + | |
- | | UANL_Mty-Mexico<sup>2</sup> || S.C.I.E.N.C.E. : Simple Code Interpretation Enabling Circuit in E. coli ||style="text-align:center;"| 7 ||style="text-align:center;"| 26 ||style="text-align:center;"| 25 ||style="text-align:center;"| 674 min
| + | |
- | |-
| + | |
- | | XMU-China<sup>3</sup> || i-ccdB: intelligent Control of Cell Density in Bacteria ||style="text-align:center;"| 3 ||style="text-align:center;"| 6 ||style="text-align:center;"| 6 ||style="text-align:center;"| < 1 min
| + | |
- | |-
| + | |
- | | NTNU-Trondheim<sup>4</sup> || Red Fluorescent Stress Sensor ||style="text-align:center;"| 2 ||style="text-align:center;"| 5 ||style="text-align:center;"| 4 ||style="text-align:center;"| < 1 min
| + | |
- | |-
| + | |
- | | Wageningen UR<sup>5</sup> || The Synchroscillator: a Synchronized Oscillatory System ||style="text-align:center;"| 4 ||style="text-align:center;"| 7 ||style="text-align:center;"| 6 ||style="text-align:center;"| < 2 min
| + | |
- | |}
| + | |
- | | + | |
- | 1 https://2011.igem.org/Team:KAIST-Korea
| + | |
- | | + | |
- | 2 https://2011.igem.org/Team:UANL_Mty-Mexico
| + | |
- | | + | |
- | 3 https://2011.igem.org/Team:XMU-China
| + | |
- | | + | |
- | 4 https://2011.igem.org/Team:NTNU_Trondheim
| + | |
- | | + | |
- | 5 https://2011.igem.org/Team:Wageningen_UR
| + | |
- | | + | |
- | <html>
| + | |
- | <p><a href="http://www.jbioleng.org/content/6/1/14/abstract" title="" class="bigbutton bPublication"><br>Our publication of The Constructor at the Journal of Biological Engineering <b>[Highly Accessed]</b>.</a></p>
| + | |
- | | + | |
- | <p><a href="https://static.igem.org/mediawiki/2012/a/a7/Poster_Constructor_A4.jpg" title="" class="bigbutton bPoster"><br>The poster of The Constructor, presented at the German Synthetic Biology Conference in May 2012 in Munich.</a></p>
| + | |
- | </html>
| + | |
- | | + | |
- | == Plug and Apply System ==
| + | |
- | | + | |
- | GFP-coil, coils, the idea
| + | |
- | | + | |
- | == Outside Modifications ==
| + | |
- | | + | |
- | * Designed the primers for amplification of the needed [[Team:Wageningen_UR/Journal/week15|constructs]]
| + | |
- | * Delivered the proof of principle that these primers work [link to framshift, Hugo]
| + | |
- | * Provided the templates for modifications and placed them in the [[Team:Wageningen_UR/Parts|partsregistry]]
| + | |
- | * Isolated the [[Team:Wageningen_UR/ObtainingthePoleroVLP|PLRV]] from nature which, in theory, should be easy to modify on the outside
| + | |
- | | + | |
- | == Inside Modifications ==
| + | |
- | | + | |
- | Negative CCMV, Frameshift
| + | |
- | | + | |
- | == Virus Like particles ==
| + | |
- | | + | |
- | Detection
| + | |
- | Isolation from nature
| + | |
- | | + | |
- | *[[RNA isolation]]
| + | |
- | *[[RT-PCR]]
| + | |
- | *[[Addition of prefix and suffix]]
| + | |
- | *[[Transformation of E.coli]]
| + | |
- | *[[Isolation of plasmids]]
| + | |
- | *[[Sequencing of PLRV CP's]]
| + | |
- | *[[Alignment experiments]]
| + | |
- | | + | |
- | == General ==
| + | |
- | | + | |
- | * We organised a [[Team:Wageningen_UR/miniSymposium|mini Symposium]] for all dutch teams in the begining of the competition to create a national network and eventualy a [[Team:Wageningen_UR/DiscoveryFestival|national effort]]
| + | |
- | * We visited [[Team:Wageningen_UR/Visiting_Secondary_School|secondary schools]] to teach them about synthetic biology and let the make leafelets about synthetic biology.
| + | |
- | * We went to present our project and [[Team:Wageningen_UR/TheConstructor|The Constructor]] at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams
| + | |
- | * We communicated to a graphical designer to obtain an kinetic graphical movie of our VLP formation, that is included in our introduction page movie.
| + | |
- | * We involved a [[Team:Wageningen_UR/DiscoveryFestival_Communication|communication study]] in our discovery festival human practises, so that we do not only inform the public, but also will have results on how scientist communicate with the public.
| + | |