Team:HKU HongKong/Project/Background.html

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<link rel="alternate" type="application/atom+xml" title="2011.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:HKU Hong Kong - 2012</title>
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&nbsp;&nbsp;Team&nbsp;&nbsp;</a>
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&nbsp;&nbsp;Project&nbsp;&nbsp;</a>
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<li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Project/Background.html">
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<li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Project/Background.html">Background</a></li></font>
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Background</a></li></font>
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<li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Project/Future_Implications.html">Future Implications</a></li></font>
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Future Implications</a></li></font>
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     <li class="topmenu"><a href="#" style="height:26px;line-height:26px;">&nbsp;&nbsp;Data&nbsp;&nbsp;</a>
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Protocols</a></li></font>
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Bio Bricks</a></li></font>
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Results</a></li></font>
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&nbsp;&nbsp;Safety&nbsp;&nbsp;</a></li>  
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&nbsp;&nbsp;Attributions&nbsp;&nbsp;</a></li>
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    <li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/Mol_Protocols.html">&nbsp;Molecular Cloning&nbsp;</a></li></font>
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<li><font size="10"><a href="https://2012.igem.org/Team:HKU_HongKong/Data/pvdQ_Protocols.html">&nbsp;pvdQ Expression Analysis&nbsp;</a></li></font>
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<h2 style="font-variant: normal; vertical-align: baseline; clear: left; color: #232323; font-family: Gentium Basic; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin: 0.7em 0px; padding: 0px">
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<h2 style="font-variant: normal; vertical-align: baseline; clear: left; color: #232323; font-family: Gentium Basic; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin: 0.7em 0px; padding: 0px">
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<span style="font-weight: 400; text-decoration:underline">
<span style="font-weight: 400; text-decoration:underline">
<font face="Trebuchet MS" size="6">Abstract</font></span></h2>
<font face="Trebuchet MS" size="6">Abstract</font></span></h2>
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advantageous because the pvdQ gene is constitutively transcribed  
advantageous because the pvdQ gene is constitutively transcribed  
regardless of environmental and endogenous factors.&nbsp; </p>
regardless of environmental and endogenous factors.&nbsp; </p>
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<p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px">
<font color="#232323">&nbsp;This synthetic genetic pathway is an auto-inductive system where pvdQ  
<font color="#232323">&nbsp;This synthetic genetic pathway is an auto-inductive system where pvdQ  
protein production will specifically depend on the presence of N-dodecanoyl-L-Homoserine  
protein production will specifically depend on the presence of N-dodecanoyl-L-Homoserine  
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regarded as a potential implication of HKU’s iGEM project.
regarded as a potential implication of HKU’s iGEM project.
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<p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px">
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    <p>&nbsp;</p>
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<u><font face="Trebuchet MS">
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<b><font size="6" color="#232323">M</font></b><font size="6" color="#232323">aterials
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&amp; </font></font><font face="Trebuchet MS" size="6" color="#232323">
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Methods</font></u></p>
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    <p>&nbsp;</p>
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<i>
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<font color="#232323">Cloning and expressing pvdQ in E. coli</font></i></p>
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<p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: #232323; font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px">pvdQ was amplified from genomic DNA of Pseudomonas
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Aeruginosa. A functional biobrick is constructed by combining pvdQ and
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some regulatory elements (such as promoter and terminator). The
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regulatory components are obtained from the iGEM Distribution Kit 2012.
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As a result, a variety of pvdQ regulatory systems can be established.
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Among the various regulation systems, the luxR regulation system is most
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concerned. luxR is a gene that can encode LuxR which binds with AHLs and
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upregulates the luxRp. As a result, in our biobrick model, the
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expression of pvdQ will be upregulated. Increase in production of pvdQ
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indicates an increase in acylase activity, which further degrades AHLs.
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The product biobrick will be a AHL-inducible acylase system. PvdQ will
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only be produced w</font></font><font size="2" color="#232323">hen AHL is present.</font></font></p>
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<p class="MsoNormal" style="text-align:justify;text-justify:inter-ideograph">
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<font color="#232323"><i>Testing the inhibitory effect</i></font></p>
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<p class="MsoNormal" style="text-align:justify;text-justify:inter-ideograph">
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<font color="#232323" face="Tahoma" size="2">The growth rate of monospecies biofilm of <i>
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Pseudomonas putida</i> is used to reflect the inhibitory effect of
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engineered <i>Escherichia coli</i>. This is because the major AHLs
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secreted by it involve 3-oxo-C12, which is a AHL that can be degraded by
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PvdQ and is also the major AHL produced in Pseudomonas areuginosa, the
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pathogenic microorganism. <i>pvdQ</i> expressing E. coli will be mixed
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with Pseudomonas putida and grown on agar plate. The reduction in
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biofilm formation will be assayed by crystal violet assay. The next part
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of the experiment is to add engineered E. coli to different
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phases of biofilm to validate the role of AHLs in biofilm formation.</font></p>
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<img border="0" src="https://static.igem.org/mediawiki/2012/1/19/Hku-logo.jpg" width="292" height="146"><img border="0" src="https://static.igem.org/mediawiki/2012/c/c7/Logo_kofa.jpg" width="406" height="98">&nbsp;&nbsp;
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+
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-
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</div>
</body>
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Latest revision as of 01:04, 27 September 2012

Team:HKU Hong Kong - 2012

Team:HKU HK

From 2011.igem.org