Team:Wisconsin-Madison

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{{Template:Wisconsin-Madison}}
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This is a template page. READ THESE INSTRUCTIONS.
 
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace. 
 
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|You can write a background of your team here. Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Wisconsin-Madison_logo.png|200px|right|frame]]
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    <div id = "divtheoverview" style="height:auto;"><br>
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      <p class = "classtheoverview"> <strong> The Translational Coupling Cassette: a tool for evaluating the translation of heterologous proteins in <i>Escherichia coli</i>. </strong></p>
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      <p align="left" class = "classtheinlinecontent2"> A powerful method for the production of novel metabolites is the expression of heterologous enzymes in a bacterial host. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion-compatible system for testing the translation of a gene of interest. This system couples the translation of the target gene to a fluorescent reporter gene; fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibodies or analytical instruments (e.g. mass spectrometry). Currently, we are utilizing this cassette to optimize the expression of limonene synthase, an enzyme that catalyzes the production of limonene, a monoterpene with potential as a renewable jet fuel.</p>
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''Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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    </div><br>
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|[[Image:Wisconsin-Madison_team.png|right|frame|Your team picture]]
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  <div style="float:left; margin-left:20px;"><a href="https://static.igem.org/mediawiki/2012/0/02/IMG_1410.jpg"> <img src="https://static.igem.org/mediawiki/2012/e/e2/UWMIMG_1411.jpeg" width="640" height="427"></a></div>
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<script src="http://widgets.twimg.com/j/2/widget.js"></script>
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|align="center"|[[Team:Wisconsin-Madison | Team Wisconsin-Madison]]
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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</p><br />
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!align="center"|[[Team:Wisconsin-Madison|Home]]
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<p align="left" class="classtheinlinecontent2"></p>
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!align="center"|[[Team:Wisconsin-Madison/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Wisconsin-Madison Official Team Profile]
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<p align="left" class="classtheinlinecontent"><strong><span style="font-size:24px"></strong>
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!align="center"|[[Team:Wisconsin-Madison/Project|Project]]
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!align="center"|[[Team:Wisconsin-Madison/Parts|Parts Submitted to the Registry]]
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</p>
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!align="center"|[[Team:Wisconsin-Madison/Modeling|Modeling]]
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<br />
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!align="center"|[[Team:Wisconsin-Madison/Notebook|Notebook]]
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<p align="left" class="classtheinlinecontent2">
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!align="center"|[[Team:Wisconsin-Madison/Safety|Safety]]
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</p><br>
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!align="center"|[[Team:Wisconsin-Madison/Attributions|Attributions]]
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Latest revision as of 20:08, 26 October 2012


The Translational Coupling Cassette: a tool for evaluating the translation of heterologous proteins in Escherichia coli.

A powerful method for the production of novel metabolites is the expression of heterologous enzymes in a bacterial host. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion-compatible system for testing the translation of a gene of interest. This system couples the translation of the target gene to a fluorescent reporter gene; fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibodies or analytical instruments (e.g. mass spectrometry). Currently, we are utilizing this cassette to optimize the expression of limonene synthase, an enzyme that catalyzes the production of limonene, a monoterpene with potential as a renewable jet fuel.









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