Team:TMU-Tokyo/Notebook/Assay

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Plasmid DNA Pure<Br>
 
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Genome DNA Purification
 
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Restruction Purification<Br>
 
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DNA Fragment Ligation<Br>
 
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Transformation<Br>
 
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Electrophoresis<Br>
 
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LB Medium<Br>
 
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<B>■Assay</B><Br>
<B>■Assay</B><Br>
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Assay0<Br>
Device1 Assay<Br>
Device1 Assay<Br>
Device2 Assay<Br>
Device2 Assay<Br>
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■Assay1(Device1)<Br></p>
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<b>■Assay0</b><Br>
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Resistant formaldehyde and formate<Br>→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_0_Protocol_and_Result">Assay 0  Protocol and Result</A><Br><Br><Br></p>
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■Assay2(Device2)<Br></p>
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<b>■Assay1(Device1)</b><Br>
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Detect formaldehyde<Br>→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_1_Protocol_and_Result">Assay 1 Protocol and Result</A><Br><Br><Br></p>
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<p class="description">
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<b>■Assay2(Device2)</b><Br>
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Resistant formaldehyde and Quantitative Analysis of Formaldehyde by Formaldehyde Test wako <Br>
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→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_2_Protocol_and_Result">Assay 2 Protocol and Result</A><Br><Br></p>
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■Assay3(Device3)<Br></p>
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<b>■Assay3(Device3)</b><Br>
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・Quantitative Analysis of formate by high performance liquid chromatography(HPLC)<Br>
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・FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺
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at 340nm.<Br>
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→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_3_Protocol_and_Result">Assay 3 Protocol and Result</A><Br><Br>
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Latest revision as of 02:15, 27 September 2012

 




■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)


■Protocols


■Assay
Assay0
Device1 Assay
Device2 Assay
Device3 Assay




Assay


■Assay0
Resistant formaldehyde and formate
→Assay 0 Protocol and Result


■Assay1(Device1)
Detect formaldehyde
→Assay 1 Protocol and Result


■Assay2(Device2)
Resistant formaldehyde and Quantitative Analysis of Formaldehyde by Formaldehyde Test wako
→Assay 2 Protocol and Result


■Assay3(Device3)
・Quantitative Analysis of formate by high performance liquid chromatography(HPLC)
・FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺ at 340nm.
→Assay 3 Protocol and Result


 

Retrieved from "http://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay"