Team:Technion/20 September 2012
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Liorlevyzzz (Talk | contribs) (Created page with "{{:Team:Technion/Template:Notebook|content= __NOTOC__ ==Ilya== ==Inbal== ==Asaf== ==Hila== ==Lior== Preparing the shipping plasmids ==Noa== Preparing the shipping plasmids ==...") |
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__NOTOC__ | __NOTOC__ | ||
==Ilya== | ==Ilya== | ||
+ | Wiki, wiki, wiki... | ||
==Inbal== | ==Inbal== | ||
- | + | - The transformation worked! I got colonies on both transformation!(of T3 and also In1 with xyIE/ALP)- so I made starters for them.. The plates were stored at 4C. | |
==Asaf== | ==Asaf== | ||
- | + | I transformed my lygated plasmid+inserts to Top 10 bacteria strain. | |
==Hila== | ==Hila== | ||
+ | - Overnight ligation to the restriction products, into pSB1C3+MCS. <br> | ||
+ | - PCR for all fragments with 100bp fragments overlap for the Gibson assembly tryout… <br> | ||
+ | - Ladder experiment's for the BEST LADDER EVERRRR!!! <br> | ||
==Lior== | ==Lior== | ||
Line 16: | Line 20: | ||
==Shahar== | ==Shahar== | ||
- | + | PCR for all fragments with 100bp fragments overlap for the Gibson assembly tryout. | |
==Rachel== | ==Rachel== | ||
+ | 4 miniprep for each RNAP(K1F, T3, N4)- at total 12 miniprep. | ||
}} | }} |
Latest revision as of 23:18, 26 September 2012
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Ilya
Wiki, wiki, wiki...
Inbal
- The transformation worked! I got colonies on both transformation!(of T3 and also In1 with xyIE/ALP)- so I made starters for them.. The plates were stored at 4C.
Asaf
I transformed my lygated plasmid+inserts to Top 10 bacteria strain.
Hila
- Overnight ligation to the restriction products, into pSB1C3+MCS.
- PCR for all fragments with 100bp fragments overlap for the Gibson assembly tryout…
- Ladder experiment's for the BEST LADDER EVERRRR!!!
Lior
Preparing the shipping plasmids
Noa
Preparing the shipping plasmids
Evgeni
Shahar
PCR for all fragments with 100bp fragments overlap for the Gibson assembly tryout.
Rachel
4 miniprep for each RNAP(K1F, T3, N4)- at total 12 miniprep.