Team:Peking/HumanPractice/Outreach/Collaboration

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  <h3 id="title1">Summary</h3>
  <h3 id="title1">Summary</h3>
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This summer, Peking 2012 iGEM team benefitted a lot from the collaboration with Prof. YANG Yi and other iGEM teams. By paying reciprocal visits with Prof. Yang, we introduced our design of Luminesensor and so got the prototype of part VVD. By help some other teams with their contests, we built lifelong friendship with team members across the world.
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This summer, our team benefitted a lot from the collaboration with Prof. YANG Yi and other iGEM teams. By paying reciprocal visits with Prof. Yang, we establish collaboration with his lab. By helping some other teams with their contests, we built lifelong friendship with team members across the world.
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  <h3 id="title2">Reciprocal visit with Prof. YANG Yi <br /> & Request for VVD</h3>
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  <h3 id="title2">Collaboration with Prof. YANG Yi's Lab</h3>
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Greatly inspired by the elaborate design of an ultrasensitive, light-controlled transactivator Gal4-VVD [1], Peking 2012 iGEM team intended to introduce a similar light-switchable transgene system into E. coli, and so invited Prof. YANG Yi from East China University of Science and Technology to visit Peking and to communicate with the team members. Later, Peking 2012 team members also made another visit to Prof. YANG Yi.
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As a burgeoning interface of biology, chemistry, physics and many other disciplines of research, the study of synthetic biology highly values and is critically depended on the collaboration of different specialities and different groups. Aiming at the optimization and further application of an ultra-sensitive light sensor, our team decided to collaborate with Prof. YANG Yi’s group from ECUST (East China University of Science and Technology) and research under the instruction of Prof. YANG.  
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The publication [1] of an ultra photosensitive, genetically encoded positive regulator, Gal4-VVD, which is rationally designed to enjoy a high spatiotemporal specificity and efficiency, greatly enlightened the Peking 2012 iGEM team on its primitive aim for programming cells to function and communicate through light.  
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Upon his visit to Peking University this May, Prof. YANG presented his design and preliminary results on the Light-OFF system and we shared our opinions on its further applications. And in this very meeting, both sides decided to combine each component of their works and present this project in the iGEM competition.  
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By sharing a mutual concern of introducing a similar system into E. coli for synthetic biological application, Prof. YANG Yi from East China University of Science and Technology and our team paid reciprocal visits to each other. During Prof. Yang’s visit to Peking University on 21st April, we shared a common view on the basic design of a LexA-VVD genetic regulator and reached a consensus for further cooperation.
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<img src="https://static.igem.org/mediawiki/igem.org/c/c1/Peking2012_HS_yangyi.JPG" alt="Prof.Yang Yi">
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Two months later, deputy team leader ZHOU Wenyuan and another team member YAN Jiawei visited Yang's lab in Shanghai to exchange ideas on experimental data and to obtain Prof. Yang’s prototype of genetic construction of the fusion protein.
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<p class="description" style="text-align:center">Professor YANG Yi</p>
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This very cooperation is not only beneficial to both sides, but also provides an opportunity for future collaborations.
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<p>Based on the original work of YANG’s group, team members in Peking University endeavored in the possible extension of this light sensitive and functional Light-OFF biobrick.  
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  <h3 id="title3">Helping other teams accomplish their projects</h3>
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  <h3 id="title3">Collaboration with Other iGEM Teams</h3>
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Another important part of iGEM is helping other teams through communication and collaboration. Team SJTU-BioX-Shanghai found out that in 2011 Peking_R team programmed ribozymes to control the expression of VioABDE gene, and so requested biobricks of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K598019"> BBa_K598019</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K598020">BBa_K598020</a> for them to incorporate in their "Membrane Workshop" project. We sent them the parts for them to use.  
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  <img src="/wiki/images/b/b2/Peking2012_HP_SJTU_logo.jpg" alt="SJTU_iGEM" style="width:200px;">
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  <p style="font-weight:bold;width:150px;text-align:center;margin:0 auto;">SJTU_iGEM</p>
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Another important part of iGEM is helping other teams through communication and collaboration. <a href="/Team:SJTU-BioX-Shanghai">Team SJTU-BioX-Shanghai</a> found that we utilized ribozymes last year to control the expression of VioABDE gene, and thereby requested biobricks of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K598019"> BBa_K598019</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K598020">BBa_K598020</a> to incorporate in their "Membrane Magic" project. We shared the DNA materials with them.
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  <img src="http://partsregistry.org/wiki/images/3/36/PekingR_ZZR2.JPG" alt="BBa_K598019" style="width:500px;">
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  <p class="description" style="width:150px;text-align:center;">BBa_K598019</p>
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  <img src="https://static.igem.org/mediawiki/igem.org/5/56/Peking2012_HS_Tinjin.png" alt="TJU_iGEM" style="width:200px;">
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  <p style="font-weight:bold;width:150px;text-align:center;margin:0 auto;">TJU_iGEM Team</p>
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  <p>We also collaborated with <a href="/Team:Tianjin">Team Tianjin</a> by sharing ideas, protocols and information. Later, we helped them to characterize their biobricks <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821001"> BBa_K821001</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821002"> BBa_K821002</a> using fluorescence stereomicroscope.
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  <img src="https://static.igem.org/mediawiki/igem.org/6/64/Peking2012_HS_OUC.jpg" alt="OUC_iGEM" style="width:200px;">
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  <p style="font-weight:bold;width:150px;text-align:center;margin:0 auto;">OUC_iGEM Team</p>
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Also we established a positive, cooperative, and steady relation with <a href="/Team:OUC-China">OUC-China</a>. Our team and OUC iGEM helped each other a lot this summer. We helped them to build stochastic model and gave some important advice about their program, which provides guidance for the experimental implementation.  Reciprocally, they shared DNA material of agarase, an enzyme that degrades agar, with us, which is very useful for our 3D bio-printing.
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  <img src="/wiki/images/4/4b/Peking2012_HP_OUC_result.jpg" alt="" style="width:500px;">
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  <p class="description" style="width:150px;text-align:center;">OUC_iGEM Result</p>
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  <img src="/wiki/images/d/dc/Peking2012_HS_SYSU.jpg" alt="SYSU_iGEM" style="width:200px;">
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  <p style="font-weight:bold;width:150px;text-align:center;margin:0 auto;">SYSU_iGEM Team</p>
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Besides, we also shared DNA materials of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K598002"> BBa_K598002</a> with <a href="/Team:SYSU-China">Team SYSU-China</a>. They exploited this digital-like and plug-in toggle switch to implement sequential logic processing in their gene circuit.
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  <img src="/wiki/images/8/8c/Peking_Collaboration_SYSU_Fluorescence_images_of_bistable_switch.png" alt="Bistable" style="width:400px;">
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  <p class="description" style="text-align:center;">Fluorescence images of bistable switch.</p>
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Latest revision as of 06:10, 24 October 2012

Summary

This summer, our team benefitted a lot from the collaboration with Prof. YANG Yi and other iGEM teams. By paying reciprocal visits with Prof. Yang, we establish collaboration with his lab. By helping some other teams with their contests, we built lifelong friendship with team members across the world.

Collaboration with Prof. YANG Yi's Lab

As a burgeoning interface of biology, chemistry, physics and many other disciplines of research, the study of synthetic biology highly values and is critically depended on the collaboration of different specialities and different groups. Aiming at the optimization and further application of an ultra-sensitive light sensor, our team decided to collaborate with Prof. YANG Yi’s group from ECUST (East China University of Science and Technology) and research under the instruction of Prof. YANG.

Upon his visit to Peking University this May, Prof. YANG presented his design and preliminary results on the Light-OFF system and we shared our opinions on its further applications. And in this very meeting, both sides decided to combine each component of their works and present this project in the iGEM competition.

Prof.Yang Yi

Professor YANG Yi

Based on the original work of YANG’s group, team members in Peking University endeavored in the possible extension of this light sensitive and functional Light-OFF biobrick.

Collaboration with Other iGEM Teams

SJTU_iGEM

SJTU_iGEM

Another important part of iGEM is helping other teams through communication and collaboration. Team SJTU-BioX-Shanghai found that we utilized ribozymes last year to control the expression of VioABDE gene, and thereby requested biobricks of BBa_K598019 and BBa_K598020 to incorporate in their "Membrane Magic" project. We shared the DNA materials with them.

BBa_K598019

BBa_K598019

TJU_iGEM

TJU_iGEM Team

We also collaborated with Team Tianjin by sharing ideas, protocols and information. Later, we helped them to characterize their biobricks BBa_K821001 and BBa_K821002 using fluorescence stereomicroscope.

OUC_iGEM

OUC_iGEM Team

Also we established a positive, cooperative, and steady relation with OUC-China. Our team and OUC iGEM helped each other a lot this summer. We helped them to build stochastic model and gave some important advice about their program, which provides guidance for the experimental implementation. Reciprocally, they shared DNA material of agarase, an enzyme that degrades agar, with us, which is very useful for our 3D bio-printing.

OUC_iGEM Result

SYSU_iGEM

SYSU_iGEM Team

Besides, we also shared DNA materials of BBa_K598002 with Team SYSU-China. They exploited this digital-like and plug-in toggle switch to implement sequential logic processing in their gene circuit.

Bistable

Fluorescence images of bistable switch.

  • Totop Totop

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