Team:Osaka/week8

From 2012.igem.org

(Difference between revisions)
(→September 16 (Sun))
 
(7 intermediate revisions not shown)
Line 17: Line 17:
==September 17 (Mon)==
==September 17 (Mon)==
 +
# Miniprep of yesterday's culture:161,162
 +
# Restriction digests of minipreppped parts
# Transfer to liquid culture:163~166,
# Transfer to liquid culture:163~166,
-
 
+
# Electrophoresis of digests
 +
# Tolerance assay(WT)
==September 18 (Tue)==
==September 18 (Tue)==
Line 24: Line 27:
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
# Gel electrophoresis of digests
# Gel electrophoresis of digests
-
# Transformation of plasmid DNA into <i>E.coli</i> HST04 161~166
+
# Transfer to liquid culture
==September 19 (Wed)==
==September 19 (Wed)==
-
# Transfer to liquid culture:161~166,
+
# Gel extraction :161,162
-
 
+
# Transfer to liquid culture:161,162
 +
# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
 +
# Electrophoresis of digests
 +
# Transformation of plasmid DNA into <i>E.coli</i>DH5α 163~166 / HST04 161,162
==September 20 (Thu)==
==September 20 (Thu)==
 +
# Miniprep of yesterday's culture:164~166
 +
# Restriction digests of minipreppped parts
 +
# Electrophoresis of digests
 +
# Tolerance assay(149,150,152,WT)
 +
# Sequencing (160~162,165,166)
 +
# Restriction digests(1-1D)
 +
# Ligation
 +
#* 167(C):1-1D(upstream)+160(downstream)+1-3A(vector)
 +
# Transformation of ligation products into DH5α <i>E.coli</i>
 +
# Transformation of plasmid DNA into <i>E.coli</i>HST04 165,166
# Preparation of glycerol stock of cell culture:161~166
# Preparation of glycerol stock of cell culture:161~166
-
 
+
# Restriction digests
 +
# Ligation
 +
#* 168(T):1-1D(upstream)+153(downstream)+1-7A(vector)
 +
#* 169(T):159upstream)+153(downstream)+1-7A(vector)
 +
#* 170(T):1-9N(upstream)+153(downstream)+1-7A(vector)
 +
#* 171(T):1-23L(upstream)+154(downstream)+1-7A(vector)
 +
#* 172(C):1-2D(upstream)+1-23L(downstream)+1-3A(vector)
 +
# Transformation of ligation products into DH5α <i>E.coli</i>
==September 21 (Fri)==
==September 21 (Fri)==
-
 
+
# Miniprep of yesterday's culture:163,164
 +
# Restriction digests of minipreped parts
 +
# Electrophoresis of digests
 +
# Tolerance assay:143~146,150
 +
# To liquid culture:WT,147,148,161~166
==September 22 (Sat)==
==September 22 (Sat)==
-
 
+
# Miniprep of yesterday's culture:163,164,167
-
 
+
# Restriction digests of minipreped parts
 +
# Electrophoresis of digests
 +
# Tolerance assay:146~148,WT
 +
# Transformation of 01,02,03,04,167 into HST04 <i>E.coli</i>
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]

Latest revision as of 02:12, 25 September 2012


September 16 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Gel extraction :161,162
  5. Ligation
    • 163(C):161(upstream)+151(downstream)+1-3A(vector)
    • 164(C):161(upstream)+152(downstream)+1-3A(vector)
    • 165(C):162(upstream)+151(downstream)+1-3A(vector)
    • 166(C):162(upstream)+152(downstream)+1-3A(vector)
  6. Transformation of ligation products into DH5α E.coli
  7. Transfer to liquid culture
  8. Tolerance assay(140,150)

September 17 (Mon)

  1. Miniprep of yesterday's culture:161,162
  2. Restriction digests of minipreppped parts
  3. Transfer to liquid culture:163~166,
  4. Electrophoresis of digests
  5. Tolerance assay(WT)

September 18 (Tue)

  1. Miniprep of yesterday's culture:163~166
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transfer to liquid culture

September 19 (Wed)

  1. Gel extraction :161,162
  2. Transfer to liquid culture:161,162
  3. Miniprep of yesterday's culture
  4. Restriction digests of minipreppped parts
  5. Electrophoresis of digests
  6. Transformation of plasmid DNA into E.coliDH5α 163~166 / HST04 161,162

September 20 (Thu)

  1. Miniprep of yesterday's culture:164~166
  2. Restriction digests of minipreppped parts
  3. Electrophoresis of digests
  4. Tolerance assay(149,150,152,WT)
  5. Sequencing (160~162,165,166)
  6. Restriction digests(1-1D)
  7. Ligation
    • 167(C):1-1D(upstream)+160(downstream)+1-3A(vector)
  8. Transformation of ligation products into DH5α E.coli
  9. Transformation of plasmid DNA into E.coliHST04 165,166
  10. Preparation of glycerol stock of cell culture:161~166
  11. Restriction digests
  12. Ligation
    • 168(T):1-1D(upstream)+153(downstream)+1-7A(vector)
    • 169(T):159upstream)+153(downstream)+1-7A(vector)
    • 170(T):1-9N(upstream)+153(downstream)+1-7A(vector)
    • 171(T):1-23L(upstream)+154(downstream)+1-7A(vector)
    • 172(C):1-2D(upstream)+1-23L(downstream)+1-3A(vector)
  13. Transformation of ligation products into DH5α E.coli

September 21 (Fri)

  1. Miniprep of yesterday's culture:163,164
  2. Restriction digests of minipreped parts
  3. Electrophoresis of digests
  4. Tolerance assay:143~146,150
  5. To liquid culture:WT,147,148,161~166


September 22 (Sat)

  1. Miniprep of yesterday's culture:163,164,167
  2. Restriction digests of minipreped parts
  3. Electrophoresis of digests
  4. Tolerance assay:146~148,WT
  5. Transformation of 01,02,03,04,167 into HST04 E.coli

Back to Notebook

Retrieved from "http://2012.igem.org/Team:Osaka/week8"