Team:LMU-Munich/Data

From 2012.igem.org

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{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo2.jpg}}
{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo2.jpg}}
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[[File:Data banner.resized WORDS.JPG|620px|link=]]
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=Data=
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[[Image:BacillusBioBrickBox.png|right|100px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
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Here you will find all of our project data.
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[[Image:BacillusBioBrickBox.png|right|100px|link=Team:LMU-Munich/Team:LMU-Munich/Bacillus_BioBricks]]
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<br>
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====Evaluation of ''B. subtilis'' vectors====
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">Seven novel vectors were constructed and four already proven to work in ''B. subtilis''.</p>
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|[[File:LacZ plate.png|right|100px|link=Team:LMU-Munich/Data/Vectors]]
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|-
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Vectors]]
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|}
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====Anderson Promoter Evaluation====
====Anderson Promoter Evaluation====
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">Evaluation of the ''Anderson'' library in ''B. subtilis'' with pSB<sub>Bs</sub>3C-''lux'' (luminescence) and pSB<sub>Bs</sub>1C-''lacZ''</p>
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|<p align="justify">Eleven Anderson promoters were measured in ''B. subtilis'' and showed relatively weak activity.</p>
|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
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====Constitutive ''Bacillus'' Promoters====
====Constitutive ''Bacillus'' Promoters====
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">Evaluation of constitutive ''B. subtilis'' promoters with pSB<sub>Bs</sub>3C-''lux'' and pSB<sub>Bs</sub>1C-''lacZ''</p>
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|<p align="justify">Three novel ''B. subtilis'' promoter BioBricks (P<sub>''liaG''</sub>, P<sub>''veg''</sub>, P<sub>''lepA''</sub>) were constructed and functionality verified by luminescence and β-galactosidase assays.</p>
|[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]]
|[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]]
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====Inducible ''Bacillus'' Promoters====
====Inducible ''Bacillus'' Promoters====
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">Evaluation of inducible ''B. subtilis'' promoters with pSB<sub>Bs</sub>3C-''lux'' and pSB<sub>Bs</sub>1C-''lacZ''</p>
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|<p align="justify">A novel antibiotic-inducible promoter, P<sub>''liaI''</sub>, was constructed and thoroughly evaluated.</p>
|[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]]
|[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]]
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===Spore purification===
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===Crust Promoter Evaluation===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells</p>
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|<p align="justify">Three sporulation-dependent promoters (P<sub>''cotYZ''</sub>, P<sub>''cotV''</sub>, P<sub>''cgeA''</sub>) were constructed, two of which show the expected behavior.</p>
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|[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/crustpromoters]]
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|}
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</div>
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===GFP-'''Sporo'''bead Evaluation===
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">They glow! GFP was successfully displayed on the spore crust as demonstrated by fluorescence microscopy.</p>
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|[[File:Sporobead Constructs Dada sheet preview.png|200px|link=Team:LMU-Munich/Data/gfp_spore]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/gfp_spore]]
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|}
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</div>
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==='''Sporo'''bead Purification===
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells: Lysozyme is the best!</p>
|[[File:Purification preview.png|200px|link=Team:LMU-Munich/Data/Sporepurification]]
|[[File:Purification preview.png|200px|link=Team:LMU-Munich/Data/Sporepurification]]
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[[File:GerminationSTOP.png|100px|right|link=Team:LMU-Munich/Germination_Stop]]
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[[File:GerminationSTOP.png|90px|right|link=Team:LMU-Munich/Germination_Stop]]
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===Knockouts of germination genes ===
===Knockouts of germination genes ===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">Germination assy of ''Bacillus'' strains with multiple gene knockouts</p>
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|<p align="justify">We successfully prevented spore germination by multiple gene knockouts.</p>
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|[[File:LMU Knockout preview.jpg|200px|link=Team:LMU-Munich/Data/Knockout]]
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|[[File:LMU Knockout previewii.jpg|200px|link=Team:LMU-Munich/Data/Knockout]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Knockout]]
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Knockout]]
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<div class="box">
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==='''Suicide'''Switch ===
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">Death upon germination: We developed a novel safety strategy as a back-up to the gene knockouts.</p>
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|[[File:PlatereaderSuicideSwitch1.jpg|200px|link=Team:LMU-Munich/Data/Suicideswitch]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Suicideswitch]]
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</div>
</div>
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[[File:LMU-Munich-Invertersign.png|90px|right|link=Team:LMU-Munich/Inverter]]
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<br>
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<div class="box" style= "background-color:#e4f1d7">
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===Inverter===
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</div>
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For our big breakthroughs, or to follow specific projects, see the individual project pages:
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===β-galactosidase assay of Inverter with ''lacZα'' ===
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|<font color="#FFFFFF">+--+--+--+--+--+--+--</font>
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|<font color="#FFFFFF">+--+--+--+--+--</font>
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|<p align="justify">It works! Invert the output of your promoter of choice.</p>
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|<font color="#FFFFFF">+--+--+--+--+--+--</font>
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|[[File:LMU Inverter graph.png|200px|link=Team:LMU-Munich/Data/Inverter]]
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|<font color="#FFFFFF">+--+--+--+--+--+--</font>
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Inverter]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
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<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=70"/>
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction"><font size="1" face="verdana">Bacillus<BR>Intro</font></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks">
 
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks"><font size="1" face="verdana">Bacillus<BR>BioBrickBOX</font></a>
 
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<div class="box" style= "background-color:#e4f1d7">
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins">
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==='''Trash'''can===
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<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=70"/>
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Things that were a pain in the ''neck''!
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins"><font size="1" face="verdana">SporeCoat<BR>FusionProteins</font></a>
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<div class="box">
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
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===''cge''A and P<sub>''cge''A</sub>===
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<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=70"/>
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">
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Unexpected restriction sites combined with no activity at all - what a bummer!</p>
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|[[File:LMU cgea preview.jpg|200px|link=Team:LMU-Munich/Data/cgeA]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop"><font size="1" face="verdana">Germination<BR>STOP</font></a>
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/cgeA]]
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<div class="box">
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===MazF===
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">
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The story of trying to clone a toxin from ''E. coli'' in ''E. coli'': They die, dammit! (...not a surprise, really...)
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</p>
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|[[File:MazFtrashcan.jpg|200px|link=Team:LMU-Munich/Data/MazF]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/MazF]]
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|}
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</div>
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<div class="box">
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===Xylose promoters===
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">
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All constructs involving xylose-inducible promoters refused cloning or evaluation (sorry, Groningen...!).
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</p>
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|[[File:100px-D-Xylose Keilstrich.png|100px|link=Team:LMU-Munich/Data/Pxyl]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Pxyl]]
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</div>
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<p></p>
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For our big breakthroughs, or to follow specific projects, see the individual project pages:
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<div class="box">
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====Project Navigation====
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{| width="100%" align="center" style="text-align:center;"
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|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
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|-
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|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
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|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
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|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
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|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
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|}
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</div>
{{:Team:LMU-Munich/Templates/Page Footer}}
{{:Team:LMU-Munich/Templates/Page Footer}}

Latest revision as of 14:31, 22 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo2.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

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