Team:LMU-Munich/Test

From 2012.igem.org

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{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo1.jpg}}
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The promoters we submit are:
+
==Our Team==
 +
{| style="color:black;" cellpadding="3" width="100%" cellspacing="0" border="0" align="center" style="text-align:left;"
 +
| style="width: 100%;background-color: #EBFCE4;" |
 +
{| style="width: 100%" align="center"
 +
|[[Image:IGEM2012_LMU_team_Korinna.jpg|Korinna|150px|center]]
 +
|[[Image:IGEM2012_LMU_team_Franzi.jpg|Franzi|150px]]
 +
|[[Image:IGEM2012_LMU_team_Tamara.jpg|Tamara|150px]]
 +
|[[Image:IGEM2012_LMU_team_Simon.jpg|Simon|150px]]
 +
|-
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Korinna
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Franzi
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Tamara
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Simon
 +
|}
 +
|-
 +
| style="width: 100%;background-color: #EBFCE4;" |
 +
{| style="width: 100%" align="center"
 +
|[[Image:IGEM2012_LMU_team_Julia.jpg|150px|center]]
 +
|[[Image:IGEM2012_LMU_team_Jenny.jpg|150px]]
 +
|[[Image:IGEM2012_LMU_team_Jara.jpg|150px]]
 +
|[[Image:IGEM2012_LMU_team_Thorsten.jpg|150px]]
 +
|-
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Julia
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Jenny
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Jara
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Thorsten
 +
|}
 +
|}
-
Pveg, PliaI, PliaG, plepA, Pxyl, Pxyl+XylR
+
| style="width: 70%;background-color: #EBFCE4;" |
 +
{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
 +
|style="width: 70%;background-color: #EBFCE4;" |
 +
<font color="#000000"; size="2">Korinna</font>
 +
|}
 +
| style="width: 70%;background-color: #EBFCE4;" |
 +
{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
 +
|style="width: 70%;background-color: #EBFCE4;" |
 +
<font color="#000000"; size="2">Franzi</font>
 +
|}
 +
|-
 +
|}
 +
|}
-
<p align="justify">We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
+
Image:IGEM2012_LMU_team_Korinna.jpg|Korinna
 +
Image:IGEM2012_LMU_team_Franzi.jpg|Franzi
 +
Image:IGEM2012_LMU_team_Tamara.jpg|Tamara
 +
Image:IGEM2012_LMU_team_Simon.jpg|Simon
 +
Image:IGEM2012_LMU_team_Julia.jpg|Julia
 +
Image:IGEM2012_LMU_team_Jenny.jpg|Jenny
 +
Image:IGEM2012_LMU_team_Jara.jpg|Jara
 +
Image:IGEM2012_LMU_team_Thorsten.jpg|Prof. Dr. Mascher
-
<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
+
|[[Image:IGEM2012_LMU_team_Julia.jpg|Julia|100px]]
 +
|[[Image:IGEM2012_LMU_team_Jenny.jpg|Jenny|100px]]
 +
|[[Image:IGEM2012_LMU_team_Jara.jpg|Jara|100px]]
 +
|[[Image:IGEM2012_LMU_team_Thorsten.jpg|Prof. Dr. Mascher|100px]]
 +
|-
 +
| style="width: 70%;background-color: #EBFCE4;" |
 +
{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
 +
|style="width: 70%;background-color: #EBFCE4;" |
 +
<font color="#000000"; size="2">''lacZ''-activity in Miller units in pSB<sub>Bs</sub>0K-P<sub>''spac''</sub> depending on ''B. subtilis'' growth phase.</font>
 +
|}
 +
|}
 +
|}
-
Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
 
 +
 +
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
 +
| style="width: 70%;background-color: #EBFCE4;" |
 +
{|
 +
|[[File:LMU-Munich-0K-OD.png|500px|center]]
 +
|-
 +
| style="width: 70%;background-color: #EBFCE4;" |
 +
{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
 +
|style="width: 70%;background-color: #EBFCE4;" |
 +
<font color="#000000"; size="2">''lacZ''-activity in Miller units in pSB<sub>Bs</sub>0K-P<sub>''spac''</sub> depending on ''B. subtilis'' growth phase.</font>
 +
|}
 +
|}
 +
|}
 +
 +
We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
 +
<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
 +
Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
More details to come soon.
More details to come soon.
<div class="box">
<div class="box">
-
{| width="100%"
+
====Project Navigation====
 +
{| width="100%" align="center" style="text-align:center;"
 +
|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
 +
|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
 +
|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
 +
|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
 +
|-
 +
|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
 +
|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
 +
|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
 +
|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
 +
|}
 +
</div>
-
| style="width:20%"|
+
 
-
'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
+
<div class="box">
-
| style="width:20%"|
+
'''Project Navigation'''
-
'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
+
{| style= cellpadding="10" width="100%" cellspacing="1" align="center" style="text-align:center;"
-
| style="width:20%"|
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|style="background-color:white"|
-
'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
+
{|
-
| style="width:20%"|
+
|style= "background-color:white"|
-
'''[[Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags|Affinity tags]]
+
[[File:Bacilluss_Intro.png|120px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction]]
 +
|style= "background-color:white"|
 +
[[Team:LMU-Munich/Bacillus_Introduction|''B. subtilis'' Intro]]
|-
|-
-
|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
+
|style= "background-color:white"|
-
|[[File:LMU PromoterIconBC.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters]]
+
[[File:BacillusBioBrickBox.png|120px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
-
|[[File:LMU Reporter.png|60px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters]]
+
|style= "background-color:white"|
-
|[[File:Proteinaffinitytagbutton.png|50px|link=Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags]]
+
[[Team:LMU-Munich/Bacillus_BioBricks|'''''Bacillus''B'''io'''B'''rick'''B'''ox]]
|-
|-
-
|valign="top"|<font size="2">
+
|}
 +
|style="background-color:white"|
 +
{|
 +
|style="background-color:white"|
 +
[[File:SporeCoat.png|100px|link=https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins]]
 +
|style="background-color:white"|
 +
[[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]]
 +
|-
 +
|style="background-color:white"|
 +
[[File:GerminationSTOP.png|90px|link=https://2012.igem.org/Team:LMU-Munich/Germination_Stop]]
 +
|style="background-color:white"|
 +
[[Team:LMU-Munich/Germination_Stop|'''Germination'''STOP]]
 +
|}
 +
|}
 +
</div>
 +
 
 +
 
 +
{|
 +
| <html><a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
 +
<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=50"/></a></html>
 +
 
 +
| [[Team:LMU-Munich/Bacillus_Introduction|Intro to ''Bacillus subtilis'']]
 +
 
 +
|-
 +
 
 +
| <html><a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks">
 +
<img src="https://static.igem.org/mediawiki/2012/7/78/BacillusBioBrickBox.png" height=50"/></a></html>
 +
 
 +
|[[Team:LMU-Munich/Bacillus_BioBricks|''Bacillus'' BioBrick'''BOX''']]
 +
 
 +
|-
 +
 
 +
|<html><a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins">
 +
<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=50"/></a></html>
 +
 
 +
|[[Team:LMU-Munich/Spore_Coat_Proteins|Sporo'''beads''']]
 +
|-
 +
 
 +
|<html><a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
 +
<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=50"/></a></html>
 +
 
 +
|[[Team:LMU-Munich/Germination_Stop|Germination'''STOP''']]
 +
|-
 +
|}
 +
 
 +
 
 +
The promoters we submit are:
 +
 
 +
Pveg, PliaI, PliaG, plepA, Pxyl, Pxyl+XylR
 +
 
 +
<p align="justify">We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
 +
 
 +
<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
 +
 
 +
Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
 +
 
 +
More details to come soon.
 +
 
 +
{| width="100%"
 +
|valign="top" style="text-align:center;"|<font size="2">
pSB<sub>Bs</sub>1C
pSB<sub>Bs</sub>1C
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|-
|-
|}
|}
-
</div>
+
 
<div class="box" style= "background-color:#e4f1d7">
<div class="box" style= "background-color:#e4f1d7">
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|}
|}
</div>
</div>
-
<p></p>
 
-
{| style="color:black;" cellpadding="10" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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<div class="box">
-
| style="width: 70%;background-color:#116f3d;" |
+
{| width="100%"
-
{|
+
 
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|[[File:Imamura, 2011 &amp; McKenney, 2010.png|Protein distribution in spore coat of ''Bacillus subtilis''|610px]]
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
-
|}
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
 +
| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
 +
| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags|Affinity tags]]
|-
|-
-
| style="width: 70%;background-color:#116f3d;" |<font color="#EBFCE4">Protein distribution in spore coat of ''Bacillus subtilis''</font>
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|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
 +
|[[File:LMU PromoterIconBC.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters]]
 +
|[[File:LMU Reporter.png|60px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters]]
 +
|[[File:Proteinaffinitytagbutton.png|50px|link=Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags]]
|-
|-
|}
|}
 +
</div>
-
We will create a toolbox of <i>Bacillus</i> BioBricks to contribute to the registry.
 
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This ''<b>Bacillus''B</b>io<b>B</b>rick<b>B</b>ox contains ''Bacillus'' specific:
 
-
{| style="background-color:white" cellpadding="0" width="100%" cellspacing="0" border="0" align="center" style="text-align:center;"
 
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| style="width:20%"|
 
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
 
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| style="width:20%"|
 
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
 
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| style="width:20%"|
 
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
 
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| style="width:20%"|
 
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Tags|Affinity tags]]
 
-
|-
 
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|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
 
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|[[File:LMU PromoterIconBC.png|100px]]
 
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|[[File:LMU Reporter.png|60px]]
 
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|[[File:Proteinaffinitytagbutton.png|50px]]
 
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|}
 
-
 
-
 
-
 
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{| class="colored"
 
-
|-
 
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!Name BioBrick
 
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!''E. coli'' res.
 
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!''B. subt.'' res.
 
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!Insertion locus
 
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!Description
 
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!Derived from
 
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!Reference
 
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|-
 
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!pSB<sub>Bs</sub>1C-<i>lacZ</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 <font color="#EBFCE4" size="2">(BBa_K823021) </font>]
 
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|Amp
 
-
|Cam
 
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|amyE
 
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|with <i>lacZ</i> reporter gene
 
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|pAC6
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke ''et al''.]
 
-
|-
 
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!pSB<sub>Bs</sub>4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 <font color="#EBFCE4" size="2">(BBa_K823022)</font>]
 
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|Amp
 
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|Spec
 
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|thrC
 
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|empty
 
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|pDG1731
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
 
-
|-
 
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!pSB<sub>Bs</sub>1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 <font color="#EBFCE4" size="2">(BBa_K823023)</font>]
 
-
|Amp
 
-
|Cam
 
-
|amyE
 
-
|empty
 
-
|pDG1662
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
 
-
|-
 
-
!pSB<sub>Bs</sub>4S-P<sub><i>Xyl</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 <font color="#EBFCE4" size="2">(BBa_K823024)</font>]
 
-
|Amp
 
-
|Spec
 
-
|thrC
 
-
|with Xylose-inducible promoter
 
-
|pXT
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré ''et al.'']
 
-
|-
 
-
!pSB<sub>Bs</sub>3C-<i>luxABCDE</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 <font color="#EBFCE4" size="2">(BBa_K823025)</font>]
 
-
|Amp
 
-
|Cam
 
-
|sacA
 
-
|with <i>luxABCDE</i> reporter cassette
 
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|pAH328
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch ''et al''.]
 
-
|-
 
-
!pSB<sub>Bs</sub>0K-P<sub><i>spac</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 <font color="#EBFCE4" size="2">(BBa_K823026)</font>]
 
-
|Amp
 
-
|Kan
 
-
|replicative
 
-
|with IPTG inducible promoter
 
-
|pDG148
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph ''et al.'']
 
-
|-
 
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!pSB<sub>Bs</sub>2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 <font color="#EBFCE4" size="2">(BBa_K823027)</font>]
 
-
|Amp
 
-
|MLS
 
-
|lacA
 
-
|empty
 
-
|pAX01
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl ''et al.'']
 
-
|-
 
-
|}
 
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Latest revision as of 22:02, 25 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo1.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Test"