Team:LMU-Munich/Test

From 2012.igem.org

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{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo1.jpg}}
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==Our Team==
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{| style="color:black;" cellpadding="3" width="100%" cellspacing="0" border="0" align="center" style="text-align:left;"
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| style="width: 100%;background-color: #EBFCE4;" |
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{| style="width: 100%" align="center"
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|[[Image:IGEM2012_LMU_team_Korinna.jpg|Korinna|150px|center]]
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|[[Image:IGEM2012_LMU_team_Franzi.jpg|Franzi|150px]]
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|[[Image:IGEM2012_LMU_team_Tamara.jpg|Tamara|150px]]
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|[[Image:IGEM2012_LMU_team_Simon.jpg|Simon|150px]]
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|-
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| style="background-color:#EBFCE4;text-align:center;" |
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Korinna
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| style="background-color:#EBFCE4;text-align:center;" |
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Franzi
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| style="background-color:#EBFCE4;text-align:center;" |
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Tamara
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| style="background-color:#EBFCE4;text-align:center;" |
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Simon
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|}
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|-
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| style="width: 100%;background-color: #EBFCE4;" |
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{| style="width: 100%" align="center"
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|[[Image:IGEM2012_LMU_team_Julia.jpg|150px|center]]
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|[[Image:IGEM2012_LMU_team_Jenny.jpg|150px]]
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|[[Image:IGEM2012_LMU_team_Jara.jpg|150px]]
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|[[Image:IGEM2012_LMU_team_Thorsten.jpg|150px]]
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|-
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| style="background-color:#EBFCE4;text-align:center;" |
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Julia
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| style="background-color:#EBFCE4;text-align:center;" |
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Jenny
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| style="background-color:#EBFCE4;text-align:center;" |
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Jara
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| style="background-color:#EBFCE4;text-align:center;" |
 +
Thorsten
 +
|}
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|}
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| style="width: 70%;background-color: #EBFCE4;" |
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{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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|style="width: 70%;background-color: #EBFCE4;" |
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<font color="#000000"; size="2">Korinna</font>
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|}
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| style="width: 70%;background-color: #EBFCE4;" |
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{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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|style="width: 70%;background-color: #EBFCE4;" |
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<font color="#000000"; size="2">Franzi</font>
 +
|}
 +
|-
 +
|}
 +
|}
 +
 +
Image:IGEM2012_LMU_team_Korinna.jpg|Korinna
 +
Image:IGEM2012_LMU_team_Franzi.jpg|Franzi
 +
Image:IGEM2012_LMU_team_Tamara.jpg|Tamara
 +
Image:IGEM2012_LMU_team_Simon.jpg|Simon
 +
Image:IGEM2012_LMU_team_Julia.jpg|Julia
 +
Image:IGEM2012_LMU_team_Jenny.jpg|Jenny
 +
Image:IGEM2012_LMU_team_Jara.jpg|Jara
 +
Image:IGEM2012_LMU_team_Thorsten.jpg|Prof. Dr. Mascher
 +
 +
|[[Image:IGEM2012_LMU_team_Julia.jpg|Julia|100px]]
 +
|[[Image:IGEM2012_LMU_team_Jenny.jpg|Jenny|100px]]
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|[[Image:IGEM2012_LMU_team_Jara.jpg|Jara|100px]]
 +
|[[Image:IGEM2012_LMU_team_Thorsten.jpg|Prof. Dr. Mascher|100px]]
 +
|-
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| style="width: 70%;background-color: #EBFCE4;" |
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{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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|style="width: 70%;background-color: #EBFCE4;" |
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<font color="#000000"; size="2">''lacZ''-activity in Miller units in pSB<sub>Bs</sub>0K-P<sub>''spac''</sub> depending on ''B. subtilis'' growth phase.</font>
 +
|}
 +
|}
 +
|}
 +
 +
 +
 +
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
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| style="width: 70%;background-color: #EBFCE4;" |
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{|
 +
|[[File:LMU-Munich-0K-OD.png|500px|center]]
 +
|-
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| style="width: 70%;background-color: #EBFCE4;" |
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{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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|style="width: 70%;background-color: #EBFCE4;" |
 +
<font color="#000000"; size="2">''lacZ''-activity in Miller units in pSB<sub>Bs</sub>0K-P<sub>''spac''</sub> depending on ''B. subtilis'' growth phase.</font>
 +
|}
 +
|}
 +
|}
 +
 +
We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
 +
<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
 +
Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
 +
More details to come soon.
 +
<div class="box">
<div class="box">
-
{| style="background-color:white" cellpadding="0" width="100%" cellspacing="0" border="0" align="center" style="text-align:center;"
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====Project Navigation====
 +
{| width="100%" align="center" style="text-align:center;"
 +
|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
 +
|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
 +
|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
 +
|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
 +
|-
 +
|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
 +
|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
 +
|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
 +
|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
 +
|}
 +
</div>
-
| style="width:20%"|
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-
'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
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<div class="box">
-
| style="width:20%"|
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'''Project Navigation'''
-
'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
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{| style= cellpadding="10" width="100%" cellspacing="1" align="center" style="text-align:center;"
-
| style="width:20%"|
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|style="background-color:white"|
-
'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
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{|
-
| style="width:20%"|
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|style= "background-color:white"|
-
'''[[Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags|Affinity tags]]
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[[File:Bacilluss_Intro.png|120px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction]]
 +
|style= "background-color:white"|
 +
[[Team:LMU-Munich/Bacillus_Introduction|''B. subtilis'' Intro]]
|-
|-
-
|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
+
|style= "background-color:white"|
-
|[[File:LMU PromoterIconBC.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters]]
+
[[File:BacillusBioBrickBox.png|120px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
-
|[[File:LMU Reporter.png|60px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters]]
+
|style= "background-color:white"|
-
|[[File:Proteinaffinitytagbutton.png|50px|link=Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags]]
+
[[Team:LMU-Munich/Bacillus_BioBricks|'''''Bacillus''B'''io'''B'''rick'''B'''ox]]
|-
|-
-
|pSB<sub>Bs</sub>3C
+
|}
 +
|style="background-color:white"|
 +
{|
 +
|style="background-color:white"|
 +
[[File:SporeCoat.png|100px|link=https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins]]
 +
|style="background-color:white"|
 +
[[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]]
 +
|-
 +
|style="background-color:white"|
 +
[[File:GerminationSTOP.png|90px|link=https://2012.igem.org/Team:LMU-Munich/Germination_Stop]]
 +
|style="background-color:white"|
 +
[[Team:LMU-Munich/Germination_Stop|'''Germination'''STOP]]
 +
|}
 +
|}
 +
</div>
-
pSB<sub>Bs</sub>3C
 
-
pSB<sub>Bs</sub>3C
+
{|
 +
| <html><a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
 +
<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=50"/></a></html>
-
pSB<sub>Bs</sub>3C
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| [[Team:LMU-Munich/Bacillus_Introduction|Intro to ''Bacillus subtilis'']]
-
|Pveg
+
 
 +
|-
 +
 
 +
| <html><a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks">
 +
<img src="https://static.igem.org/mediawiki/2012/7/78/BacillusBioBrickBox.png" height=50"/></a></html>
 +
 
 +
|[[Team:LMU-Munich/Bacillus_BioBricks|''Bacillus'' BioBrick'''BOX''']]
 +
 
 +
|-
 +
 
 +
|<html><a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins">
 +
<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=50"/></a></html>
 +
 
 +
|[[Team:LMU-Munich/Spore_Coat_Proteins|Sporo'''beads''']]
 +
|-
 +
 
 +
|<html><a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
 +
<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=50"/></a></html>
 +
 
 +
|[[Team:LMU-Munich/Germination_Stop|Germination'''STOP''']]
 +
|-
 +
|}
 +
 
 +
 
 +
The promoters we submit are:
 +
 
 +
Pveg, PliaI, PliaG, plepA, Pxyl, Pxyl+XylR
 +
 
 +
<p align="justify">We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
 +
 
 +
<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
 +
 
 +
Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
 +
 
 +
More details to come soon.
 +
 
 +
{| width="100%"
 +
|valign="top" style="text-align:center;"|<font size="2">
 +
pSB<sub>Bs</sub>1C
 +
 
 +
pSB<sub>Bs</sub>4S
 +
 
 +
pSB<sub>Bs</sub>2E
 +
 
 +
pSB<sub>Bs</sub>1C-''lac''Z
 +
 
 +
pSB<sub>Bs</sub>3C-''lux''ABCDE
 +
 
 +
pSB<sub>Bs</sub>4S-P<sub>''Xyl''</sub>
 +
 
 +
pSB<sub>Bs</sub>0K-P<sub>''spac''</sub>
 +
</font>
 +
|valign="top"|<font size="2">
 +
Pveg
PliaI
PliaI
Line 33: Line 209:
Anderson
Anderson
-
|
+
 
 +
</font>
 +
|valign="top"|<font size="2">
mkate2
mkate2
Line 39: Line 217:
lacZ
lacZ
-
|
 
 +
</font>
 +
|valign="top"|<font size="2">
Flag
Flag
Line 46: Line 225:
cMyc
cMyc
 +
 +
Strep
 +
 +
HA
 +
</font>
|-
|-
|}
|}
-
</div>
+
 
<div class="box" style= "background-color:#e4f1d7">
<div class="box" style= "background-color:#e4f1d7">
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|<p align="justify">Evaluation of the Anderson library in ''B. subtilis'' with pSB<sub>Bs</sub>3C-''lux'' (luminescence) and pSB<sub>Bs</sub>1C-''lacZ''
|<p align="justify">Evaluation of the Anderson library in ''B. subtilis'' with pSB<sub>Bs</sub>3C-''lux'' (luminescence) and pSB<sub>Bs</sub>1C-''lacZ''
[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Anderson]]</p>
[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Anderson]]</p>
 +
|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
 +
|}
 +
</div>
 +
 +
<div class="box">
 +
====Anderson Promoter Evaluation====
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">Evaluation of the Anderson library in ''B. subtilis'' with pSB<sub>Bs</sub>3C-''lux'' (luminescence) and pSB<sub>Bs</sub>1C-''lacZ''</p>
|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
|-
|-
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|}
|}
</div>
</div>
-
<p></p>
 
-
{| style="color:black;" cellpadding="10" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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<div class="box">
-
| style="width: 70%;background-color:#116f3d;" |
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{| width="100%"
-
{|
+
 
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|[[File:Imamura, 2011 &amp; McKenney, 2010.png|Protein distribution in spore coat of ''Bacillus subtilis''|610px]]
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
-
|}
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
 +
| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
 +
| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags|Affinity tags]]
|-
|-
-
| style="width: 70%;background-color:#116f3d;" |<font color="#EBFCE4">Protein distribution in spore coat of ''Bacillus subtilis''</font>
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|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
 +
|[[File:LMU PromoterIconBC.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters]]
 +
|[[File:LMU Reporter.png|60px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters]]
 +
|[[File:Proteinaffinitytagbutton.png|50px|link=Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags]]
|-
|-
|}
|}
 +
</div>
-
We will create a toolbox of <i>Bacillus</i> BioBricks to contribute to the registry.
 
-
This ''<b>Bacillus''B</b>io<b>B</b>rick<b>B</b>ox contains ''Bacillus'' specific:
 
-
{| style="background-color:white" cellpadding="0" width="100%" cellspacing="0" border="0" align="center" style="text-align:center;"
 
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| style="width:20%"|
 
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
 
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| style="width:20%"|
 
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
 
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| style="width:20%"|
 
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
 
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| style="width:20%"|
 
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Tags|Affinity tags]]
 
-
|-
 
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|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
 
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|[[File:LMU PromoterIconBC.png|100px]]
 
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|[[File:LMU Reporter.png|60px]]
 
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|[[File:Proteinaffinitytagbutton.png|50px]]
 
-
|}
 
-
 
-
 
-
 
-
 
-
{| class="colored"
 
-
|-
 
-
!Name BioBrick
 
-
!''E. coli'' res.
 
-
!''B. subt.'' res.
 
-
!Insertion locus
 
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!Description
 
-
!Derived from
 
-
!Reference
 
-
|-
 
-
!pSB<sub>Bs</sub>1C-<i>lacZ</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 <font color="#EBFCE4" size="2">(BBa_K823021) </font>]
 
-
|Amp
 
-
|Cam
 
-
|amyE
 
-
|with <i>lacZ</i> reporter gene
 
-
|pAC6
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke ''et al''.]
 
-
|-
 
-
!pSB<sub>Bs</sub>4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 <font color="#EBFCE4" size="2">(BBa_K823022)</font>]
 
-
|Amp
 
-
|Spec
 
-
|thrC
 
-
|empty
 
-
|pDG1731
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
 
-
|-
 
-
!pSB<sub>Bs</sub>1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 <font color="#EBFCE4" size="2">(BBa_K823023)</font>]
 
-
|Amp
 
-
|Cam
 
-
|amyE
 
-
|empty
 
-
|pDG1662
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
 
-
|-
 
-
!pSB<sub>Bs</sub>4S-P<sub><i>Xyl</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 <font color="#EBFCE4" size="2">(BBa_K823024)</font>]
 
-
|Amp
 
-
|Spec
 
-
|thrC
 
-
|with Xylose-inducible promoter
 
-
|pXT
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré ''et al.'']
 
-
|-
 
-
!pSB<sub>Bs</sub>3C-<i>luxABCDE</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 <font color="#EBFCE4" size="2">(BBa_K823025)</font>]
 
-
|Amp
 
-
|Cam
 
-
|sacA
 
-
|with <i>luxABCDE</i> reporter cassette
 
-
|pAH328
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch ''et al''.]
 
-
|-
 
-
!pSB<sub>Bs</sub>0K-P<sub><i>spac</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 <font color="#EBFCE4" size="2">(BBa_K823026)</font>]
 
-
|Amp
 
-
|Kan
 
-
|replicative
 
-
|with IPTG inducible promoter
 
-
|pDG148
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph ''et al.'']
 
-
|-
 
-
!pSB<sub>Bs</sub>2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 <font color="#EBFCE4" size="2">(BBa_K823027)</font>]
 
-
|Amp
 
-
|MLS
 
-
|lacA
 
-
|empty
 
-
|pAX01
 
-
|[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl ''et al.'']
 
-
|-
 
-
|}
 
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Latest revision as of 22:02, 25 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo1.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Test"