Team:Technion/18 September 2012
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__NOTOC__ | __NOTOC__ | ||
==Ilya== | ==Ilya== | ||
- | - Worked a bit more on the wiki design. | + | - Worked a bit more on the wiki design.<br> |
+ | - Worked on the RBS calculator results for the riboswitch. | ||
==Inbal== | ==Inbal== | ||
- | + | Today I made 6 starters: 3 of them is the whole part (In1) and the rest is T3 RNAP (within pSB1C3) from our glycerol stock.<br> | |
+ | I also cleaned by a kit the cip reaction from the Sep 15. The concentration is 66ng/ul. | ||
==Asaf== | ==Asaf== | ||
+ | I ran the restriction fragments I got from cutting the differnet inserts on gel.<br> | ||
+ | I got the 3 kb band I expected and cut it from the gel and cleaned it.<br> | ||
+ | <gallery perrow="4" widths=150px heights=150px style="color: black; border: 5px ridge #A7A3BF; font: 13px david; background: azure"> | ||
+ | Image:18.9_RS+poly-EcoRI+PstI_gel_extraction_up-view.jpg | ||
+ | Image:18.9_RS+poly-EcoRI+PstI_gel_extraction_down-view.jpg | ||
+ | </gallery> | ||
==Hila== | ==Hila== | ||
- | + | - PCR for F3 with 400bp overlap, this fragment will use in the three fragments Gibson assembly. <br> | |
==Lior== | ==Lior== | ||
Line 19: | Line 27: | ||
==Rachel== | ==Rachel== | ||
+ | Transformation after ligation of K1F+ter, T3+ter, N4+ter (within pSB1AK3 plasmid) and control (plasmid after cip treatment)to the TOP10 component cells on AMP antibiotics plates. | ||
}} | }} |
Latest revision as of 11:55, 26 September 2012
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Ilya
- Worked a bit more on the wiki design.
- Worked on the RBS calculator results for the riboswitch.
Inbal
Today I made 6 starters: 3 of them is the whole part (In1) and the rest is T3 RNAP (within pSB1C3) from our glycerol stock.
I also cleaned by a kit the cip reaction from the Sep 15. The concentration is 66ng/ul.
Asaf
I ran the restriction fragments I got from cutting the differnet inserts on gel.
I got the 3 kb band I expected and cut it from the gel and cleaned it.
Hila
- PCR for F3 with 400bp overlap, this fragment will use in the three fragments Gibson assembly.
Lior
Noa
Evgeni
Shahar
Rachel
Transformation after ligation of K1F+ter, T3+ter, N4+ter (within pSB1AK3 plasmid) and control (plasmid after cip treatment)to the TOP10 component cells on AMP antibiotics plates.