Team:WashU/Protocols/PCR

From 2012.igem.org

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__NOTOC__
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<!--Thanks to UW iGEM '11 for the above instructions.  The original source can be found here: https://2011.igem.org/Team:Washington/Protocols/PCR-->
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{{WashUbackprotocols}}
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<div id = "protocolshort">
=General PCR Protocol=
=General PCR Protocol=
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#Set up PCR reaction tube
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==Set up PCR reaction tube==
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##1uL Template
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#1uL Template
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##1uL 25mM dNTP's
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#1uL 25mM dNTP's
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##10uL Phusion HF Buffer
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#10uL Buffer
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##0.5uL Forward Primer (Tm XX <sup>o</sup>C)*
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#0.5uL Forward Primer
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##0.5uL Reverse Primer (Tm YY <sup>o</sup>C)*
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#0.5uL Reverse Primer
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##0.5uL TAQ polymerase
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#0.5uL TAQ polymerase
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##36.5uL diH2O
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#36.5uL diH2O
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#Amplification reaction in thermocycler depends
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==Amplification reaction in thermocycler==
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#1 cycle 94 degrees (3 minutes)
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#40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
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[https://2012.igem.org/Team:WashU/Protocols Back to Protocols]

Latest revision as of 21:30, 9 August 2012


General PCR Protocol

Set up PCR reaction tube

  1. 1uL Template
  2. 1uL 25mM dNTP's
  3. 10uL Buffer
  4. 0.5uL Forward Primer
  5. 0.5uL Reverse Primer
  6. 0.5uL TAQ polymerase
  7. 36.5uL diH2O

Amplification reaction in thermocycler

  1. 1 cycle 94 degrees (3 minutes)
  2. 40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
Retrieved from "http://2012.igem.org/Team:WashU/Protocols/PCR"