Team:Exeter/lab book/proto/2

From 2012.igem.org

(Difference between revisions)
(Created page with "{{Template:Team:Exeter/e-candi_banner}} <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <title>Protocol ...")
 
(One intermediate revision not shown)
Line 61: Line 61:
     <li><p>Defrost antibiotic for selection, corresponding to the plate antibiotic. Add antibiotic to 5ml of LB broth corresponding to a 1000 fold dilution.</p></li>
     <li><p>Defrost antibiotic for selection, corresponding to the plate antibiotic. Add antibiotic to 5ml of LB broth corresponding to a 1000 fold dilution.</p></li>
     <li><p>Select three colonies from each plate and inoculate to 5ml of LB media via a pipette tip.</p></li>
     <li><p>Select three colonies from each plate and inoculate to 5ml of LB media via a pipette tip.</p></li>
 +
    <li><p>Incubate cultures overnight in a shaking incubator ~225 rpm, 37 °C.</p></li>
     </ul>   
     </ul>   
   </font>
   </font>
Line 67: Line 68:
</table>
</table>
-
 
+
<table width="980" align="center" cellspacing="20">
 +
<tr align="center">
 +
  <td>
 +
  <font color="#57B947" size="1" face="Verdana">
 +
    <p><u>Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley</u> &nbsp;&nbsp;|&nbsp;&nbsp;
 +
    <a href="https://igem.org/Team.cgi?id=764" style="color:#57B947" target="_blank"><u>Contact Us</u></a>  &nbsp;&nbsp;|&nbsp;&nbsp;
 +
    <a href="https://2012.igem.org/Team:Exeter/site_map" style="color:#57B947"><u>Site Map</u></a></p>
 +
  </font>
 +
  </td>
 +
</tr>
 +
</table>
</body>
</body>
</html>
</html>

Latest revision as of 23:47, 26 September 2012

Protocol 2

Transferring Cultures to Liquid Medium

  • Defrost antibiotic for selection, corresponding to the plate antibiotic. Add antibiotic to 5ml of LB broth corresponding to a 1000 fold dilution.

  • Select three colonies from each plate and inoculate to 5ml of LB media via a pipette tip.

  • Incubate cultures overnight in a shaking incubator ~225 rpm, 37 °C.

Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley   |   Contact Us   |   Site Map

Retrieved from "http://2012.igem.org/Team:Exeter/lab_book/proto/2"