Team:Nanjing-China

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                <p>Nanjing-China 2012 is working on small interfering genetically engineered machine.</p>
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                  <h4>First Thumbnail label</h4>
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                   <p>Our team</p>
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                   <p>test1</p>
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                   <p>Engineered lettuce growing</p>
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                   <p>test2</p>
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           <h2>Abstract</h2>
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          <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
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          <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
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          <h2>siGEM at a glance</h2>
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          <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
+
          <p class="MsoNormal"><span lang="EN-US">The essential and expanding roles of
-
        </div>
+
small non coding RNAs are becoming clearer to us. More interestingly, plant
 +
endogenous small RNAs through food intake directly influence gene expression in
 +
animals (especially in liver) , as a recent study showed.&nbsp;&nbsp; </span></p>
 +
<p class="MsoNormal"><span lang="EN-US">We took advantage of this
 +
<span class="word">phenomenon</span>, and made engineered lettuce producing
 +
artificial small RNA for therapeutic purpose. We in this case chose PGC1-alpha,  
 +
whose high expression in liver can cause insulin resistance, as target. (More...
 +
in project section)</span></p>          </div>
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          <h2>Parts at a glance</h2>
 +
          <p class="MsoNormal"><span lang="EN-US">Our team designed three main modules for
 +
our project, si/mi RNA and green plant synthetic biology. They may serve as a  
 +
bit of ground work for future teams working on plant.</span></p>
 +
<p class="MsoNormal"><span lang="EN-US">They are RNA generating module, T-DNA
 +
module, and Barcode 2 module. </span></p>
 +
<p class="MsoNormal"><span lang="EN-US">&nbsp;RNA generating module make vegetable a  
 +
small RNA producing factory.</span></p>
 +
<p class="MsoNormal"><span lang="EN-US">Parts in T-DNA module are used for
 +
constructing customized binary vector for plant.</span></p>
 +
<p class="MsoNormal"><span lang="EN-US">Barcode 2 module is a signature system
 +
for engineered plant.</span></p>
 +
<p class="MsoNormal"><span lang="EN-US">&nbsp;</span></p>
 +
<p class="MsoNormal"><span lang="EN-US">(Read more in Parts and project)</span></p>
 +
 
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          <h2>Sharing</h2>
 +
          <p class="MsoNormal"><span lang="EN-US">You can simply use overlapping PCR to  
 +
switch the sequence of small RNAs in part BBa_K788002 to make your own parts and  
 +
plant. (Detail is available in the part registry
 +
<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788002">
 +
BBa_K788002</a>). Also, you can use part<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788010">
 +
BBa_K788010 </a>and<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788005">
 +
BBa_K7880005</a> to make new binary vectors. And you may put the signature on
 +
the plant you work with by adding a barcode 2 part at the end of your system
 +
according to rules
 +
<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788007">
 +
BBa_K880007</a> gave.</span></p>         </div>
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Latest revision as of 00:13, 27 September 2012

siGEM at a glance

The essential and expanding roles of small non coding RNAs are becoming clearer to us. More interestingly, plant endogenous small RNAs through food intake directly influence gene expression in animals (especially in liver) , as a recent study showed.  

We took advantage of this phenomenon, and made engineered lettuce producing artificial small RNA for therapeutic purpose. We in this case chose PGC1-alpha, whose high expression in liver can cause insulin resistance, as target. (More... in project section)

Parts at a glance

Our team designed three main modules for our project, si/mi RNA and green plant synthetic biology. They may serve as a bit of ground work for future teams working on plant.

They are RNA generating module, T-DNA module, and Barcode 2 module.

 RNA generating module make vegetable a small RNA producing factory.

Parts in T-DNA module are used for constructing customized binary vector for plant.

Barcode 2 module is a signature system for engineered plant.

 

(Read more in Parts and project)

Sharing

You can simply use overlapping PCR to switch the sequence of small RNAs in part BBa_K788002 to make your own parts and plant. (Detail is available in the part registry BBa_K788002). Also, you can use part BBa_K788010 and BBa_K7880005 to make new binary vectors. And you may put the signature on the plant you work with by adding a barcode 2 part at the end of your system according to rules BBa_K880007 gave.

Retrieved from "http://2012.igem.org/Team:Nanjing-China"