Team:Groningen/Notebook/Wetwork 11June2012

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Picking our bio-bricks (Renske, Tom, Yonathan)
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Complete construct design (Nisa)
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Start transformation
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Nisa: designed primers for construct with pigments (lycopene, violacein) from Cambrigde 2008. Further busy with design/literature study of positive feedback loop. <br><br>
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Literature study: search for promoter.<br><br>
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Transformed Violacein (BBa_K274002) into e.coli dh5a: <br>
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*2 uL of DNA into 50-75 uL of cells<br>
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*30 min on ice <br>
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*2 min, 42 degrees C <br>
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*back on ice, add 300 uL LB <br>
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*1.5 hour in 37 degrees C <br>
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*plate (LB with 10 ug/mL chloramphenicol), O/N at 37 degrees C
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 16:38, 25 September 2012







Nisa: designed primers for construct with pigments (lycopene, violacein) from Cambrigde 2008. Further busy with design/literature study of positive feedback loop.

Literature study: search for promoter.

Transformed Violacein (BBa_K274002) into e.coli dh5a:
*2 uL of DNA into 50-75 uL of cells
*30 min on ice
*2 min, 42 degrees C
*back on ice, add 300 uL LB
*1.5 hour in 37 degrees C
*plate (LB with 10 ug/mL chloramphenicol), O/N at 37 degrees C
Back to notebook



Our sponsors:

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