Team:LMU-Munich/Test

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Latest revision as of 22:02, 25 September 2012

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

[ more news ]

Our Team


Korinna	Franzi	Tamara	Simon


Julia	Jenny	Jara	Thorsten

| style="width: 70%;background-color: #EBFCE4;" |

Korinna

| style="width: 70%;background-color: #EBFCE4;" |

Franzi

|- |} |}

| | | | |- | style="width: 70%;background-color: #EBFCE4;" |

lacZ-activity in Miller units in pSB_Bs0K-P_spac depending on B. subtilis growth phase.

|} |}

lacZ-activity in Miller units in pSB_Bs0K-P_spac depending on B. subtilis growth phase.

We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in Bacillus subtilis with pSB_Bs3C-luxABCDE. The data will be online in our Data section soon.</p>

Furthermore we plan to submit reporter genes optimized for Bacillus subtilis, namely GFP, lacZ, luc and mKate2.

Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA). More details to come soon.

Project Navigation


Bacillus Intro	Bacillus BioBrickBox	Sporobeads	Germination STOP

Project Navigation

	B. subtilis Intro
	BacillusBioBrickBox

	Sporobeads
	GerminationSTOP

	Intro to Bacillus subtilis
	Bacillus BioBrickBOX
	Sporobeads
	GerminationSTOP

The promoters we submit are:

Pveg, PliaI, PliaG, plepA, Pxyl, Pxyl+XylR

Furthermore we plan to submit reporter genes optimized for Bacillus subtilis, namely GFP, lacZ, luc and mKate2.

Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).

More details to come soon.

pSB_Bs1C

pSB_Bs4S

pSB_Bs2E

pSB_Bs1C-lacZ

pSB_Bs3C-luxABCDE

pSB_Bs4S-P_Xyl

pSB_Bs0K-P_spac

Pveg

PliaI

PlepA

Anderson

mkate2

luc

lacZ

Flag

His

cMyc

Strep

BacillusBioBrickBox

Anderson Promoter Evaluation

Evaluation of the Anderson library in B. subtilis with pSB_Bs3C-lux (luminescence) and pSB_Bs1C-lacZ

Anderson Promoter Evaluation


Evaluation of the Anderson library in B. subtilis with pSB_Bs3C-lux (luminescence) and pSB_Bs1C-lacZ

Vectors	Promoters	Reporters	Affinity tags

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Test"

@@ Line 2: / Line 2: @@
 {{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo1.jpg}}
+==Our Team==
+{| style="color:black;" cellpadding="3" width="100%" cellspacing="0" border="0" align="center" style="text-align:left;"
+| style="width: 100%;background-color: #EBFCE4;" |
+{| style="width: 100%" align="center"
+|[[Image:IGEM2012_LMU_team_Korinna.jpg|Korinna|150px|center]]
+|[[Image:IGEM2012_LMU_team_Franzi.jpg|Franzi|150px]]
+|[[Image:IGEM2012_LMU_team_Tamara.jpg|Tamara|150px]]
+|[[Image:IGEM2012_LMU_team_Simon.jpg|Simon|150px]]
+|-
+| style="background-color:#EBFCE4;text-align:center;" |
+Korinna
+| style="background-color:#EBFCE4;text-align:center;" |
+Franzi
+| style="background-color:#EBFCE4;text-align:center;" |
+Tamara
+| style="background-color:#EBFCE4;text-align:center;" |
+Simon
+|}
+|-
+| style="width: 100%;background-color: #EBFCE4;" |
+{| style="width: 100%" align="center"
+|[[Image:IGEM2012_LMU_team_Julia.jpg|150px|center]]
+|[[Image:IGEM2012_LMU_team_Jenny.jpg|150px]]
+|[[Image:IGEM2012_LMU_team_Jara.jpg|150px]]
+|[[Image:IGEM2012_LMU_team_Thorsten.jpg|150px]]
+|-
+| style="background-color:#EBFCE4;text-align:center;" |
+Julia
+| style="background-color:#EBFCE4;text-align:center;" |
+Jenny
+| style="background-color:#EBFCE4;text-align:center;" |
+Jara
+| style="background-color:#EBFCE4;text-align:center;" |
+Thorsten
+|}
+|}
+| style="width: 70%;background-color: #EBFCE4;" |
+{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
+|style="width: 70%;background-color: #EBFCE4;" |
+<font color="#000000"; size="2">Korinna</font>
+|}
+| style="width: 70%;background-color: #EBFCE4;" |
+{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
+|style="width: 70%;background-color: #EBFCE4;" |
+<font color="#000000"; size="2">Franzi</font>
+|}
+|-
+|}
+|}
-We will create a toolbox of <i>Bacillus</i> BioBricks to contribute to the registry.
+Image:IGEM2012_LMU_team_Korinna.jpg|Korinna
+Image:IGEM2012_LMU_team_Franzi.jpg|Franzi
+Image:IGEM2012_LMU_team_Tamara.jpg|Tamara
+Image:IGEM2012_LMU_team_Simon.jpg|Simon
+Image:IGEM2012_LMU_team_Julia.jpg|Julia
+Image:IGEM2012_LMU_team_Jenny.jpg|Jenny
+Image:IGEM2012_LMU_team_Jara.jpg|Jara
+Image:IGEM2012_LMU_team_Thorsten.jpg|Prof. Dr. Mascher
-This ''<b>Bacillus''B</b>io<b>B</b>rick<b>B</b>ox contains ''Bacillus'' specific:
+|[[Image:IGEM2012_LMU_team_Julia.jpg|Julia|100px]]
+|[[Image:IGEM2012_LMU_team_Jenny.jpg|Jenny|100px]]
+|[[Image:IGEM2012_LMU_team_Jara.jpg|Jara|100px]]
+|[[Image:IGEM2012_LMU_team_Thorsten.jpg|Prof. Dr. Mascher|100px]]
+|-
+| style="width: 70%;background-color: #EBFCE4;" |
+{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
+|style="width: 70%;background-color: #EBFCE4;" |
+<font color="#000000"; size="2">''lacZ''-activity in Miller units in pSB<sub>Bs</sub>0K-P<sub>''spac''</sub> depending on ''B. subtilis'' growth phase.</font>
+|}
+|}
+|}
-{| style="background-color:white" cellpadding="10" width="100%" cellspacing="1" border="0" align="center" style="text-align:center;"
+{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
+| style="width: 70%;background-color: #EBFCE4;" |
+{|
+|[[File:LMU-Munich-0K-OD.png|500px|center]]
+|-
+| style="width: 70%;background-color: #EBFCE4;" |
+{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
+|style="width: 70%;background-color: #EBFCE4;" |
+<font color="#000000"; size="2">''lacZ''-activity in Miller units in pSB<sub>Bs</sub>0K-P<sub>''spac''</sub> depending on ''B. subtilis'' growth phase.</font>
+|}
+|}
+|}
+We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
+<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
+Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
+More details to come soon.
-|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
+<div class="box">
-|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
+====Project Navigation====
-|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
+{| width="100%" align="center" style="text-align:center;"
-|'''[[Team:LMU-Munich/Bacillus_BioBricks#Tags|Tags]]
+|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
+|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
+|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
+|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
 |-
-|[[File:LMU Backbone.png|200pxlink=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
+|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
-|[[File:LMU PromoterIconBC.png|200px]]
+|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
-|[[File:LMU Reporter.png|100px]]
+|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
-|[[File:Proteinaffinitytagbutton.png|100px]]
+|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
 |}
+</div>
+<div class="box">
+'''Project Navigation'''
+{| style= cellpadding="10" width="100%" cellspacing="1" align="center" style="text-align:center;"
+|style="background-color:white"|
+{|
+|style= "background-color:white"|
+[[File:Bacilluss_Intro.png|120px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction]]
+|style= "background-color:white"|
+[[Team:LMU-Munich/Bacillus_Introduction|''B. subtilis'' Intro]]
+|-
+|style=  "background-color:white"|
+[[File:BacillusBioBrickBox.png|120px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
+|style= "background-color:white"|
+[[Team:LMU-Munich/Bacillus_BioBricks|'''''Bacillus''B'''io'''B'''rick'''B'''ox]]
+|-
+|}
+|style="background-color:white"|
+{|
+|style="background-color:white"|
+[[File:SporeCoat.png|100px|link=https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins]]
+|style="background-color:white"|
+[[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]]
+|-
+|style="background-color:white"|
+[[File:GerminationSTOP.png|90px|link=https://2012.igem.org/Team:LMU-Munich/Germination_Stop]]
+|style="background-color:white"|
+[[Team:LMU-Munich/Germination_Stop|'''Germination'''STOP]]
+|}
+|}
+</div>
+{|
+| <html><a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
+<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=50"/></a></html>
+| [[Team:LMU-Munich/Bacillus_Introduction|Intro to ''Bacillus subtilis'']]
-{| class="colored"
-|-
-!Name BioBrick
-!''E. coli'' res.
-!''B. subt.'' res.
-!Insertion locus
-!Description
-!Derived from
-!Reference
 |-
-!pSB<sub>Bs</sub>1C-<i>lacZ</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 <font color="#EBFCE4" size="2">(BBa_K823021) </font>]
-|Amp
+| <html><a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks">
-|Cam
+<img src="https://static.igem.org/mediawiki/2012/7/78/BacillusBioBrickBox.png" height=50"/></a></html>
-|amyE
-|with <i>lacZ</i> reporter gene
+|[[Team:LMU-Munich/Bacillus_BioBricks|''Bacillus'' BioBrick'''BOX''']]
-|pAC6
-|[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke ''et al''.]
 |-
-!pSB<sub>Bs</sub>4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 <font color="#EBFCE4" size="2">(BBa_K823022)</font>]
-|Amp
+|<html><a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins">
-|Spec
+<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=50"/></a></html>
-|thrC
-|empty
+|[[Team:LMU-Munich/Spore_Coat_Proteins|Sporo'''beads''']]
-|pDG1731
-|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
 |-
-!pSB<sub>Bs</sub>1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 <font color="#EBFCE4" size="2">(BBa_K823023)</font>]
-|Amp
+|<html><a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
-|Cam
+<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=50"/></a></html>
-|amyE
-|empty
+|[[Team:LMU-Munich/Germination_Stop|Germination'''STOP''']]
-|pDG1662
-|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
 |-
-!pSB<sub>Bs</sub>4S-P<sub><i>Xyl</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 <font color="#EBFCE4" size="2">(BBa_K823024)</font>]
+|}
-|Amp
-|Spec
-|thrC
+The promoters we submit are:
-|with Xylose-inducible promoter
-|pXT
+Pveg, PliaI, PliaG, plepA, Pxyl, Pxyl+XylR
-|[http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré ''et al.'']
+<p align="justify">We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
+<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
+Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
+More details to come soon.
+{| width="100%"
+|valign="top" style="text-align:center;"|<font size="2">
+pSB<sub>Bs</sub>1C
+pSB<sub>Bs</sub>4S
+pSB<sub>Bs</sub>2E
+pSB<sub>Bs</sub>1C-''lac''Z
+pSB<sub>Bs</sub>3C-''lux''ABCDE
+pSB<sub>Bs</sub>4S-P<sub>''Xyl''</sub>
+pSB<sub>Bs</sub>0K-P<sub>''spac''</sub>
+</font>
+|valign="top"|<font size="2">
+Pveg
+PliaI
+PlepA
+Anderson
+</font>
+|valign="top"|<font size="2">
+mkate2
+luc
+lacZ
+</font>
+|valign="top"|<font size="2">
+Flag
+His
+cMyc
+Strep
+HA
+</font>
 |-
-!pSB<sub>Bs</sub>3C-<i>luxABCDE</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 <font color="#EBFCE4" size="2">(BBa_K823025)</font>]
+|}
-|Amp
-|Cam
-|sacA
+<div class="box" style= "background-color:#e4f1d7">
-|with <i>luxABCDE</i> reporter cassette
+==='''''Bacillus''B'''io'''B'''rick'''B'''ox===
-|pAH328
+</div>
-|[http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch ''et al''.]
+<div class="box">
+====Anderson Promoter Evaluation====
+{| "width=100%" style="text-align:center;"|
+|<p align="justify">Evaluation of the Anderson library in ''B. subtilis'' with pSB<sub>Bs</sub>3C-''lux'' (luminescence) and pSB<sub>Bs</sub>1C-''lacZ''
+[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Anderson]]</p>
+|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
+|}
+</div>
+<div class="box">
+====Anderson Promoter Evaluation====
+{| "width=100%" style="text-align:center;"|
+|<p align="justify">Evaluation of the Anderson library in ''B. subtilis'' with pSB<sub>Bs</sub>3C-''lux'' (luminescence) and pSB<sub>Bs</sub>1C-''lacZ''</p>
+|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
 |-
-!pSB<sub>Bs</sub>0K-P<sub><i>spac</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 <font color="#EBFCE4" size="2">(BBa_K823026)</font>]
+! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Anderson]]
-|Amp
+|}
-|Kan
+</div>
-|replicative
-|with IPTG inducible promoter
+<div class="box">
-|pDG148
+{| width="100%"
-|[http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph ''et al.'']
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags|Affinity tags]]
 |-
-!pSB<sub>Bs</sub>2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 <font color="#EBFCE4" size="2">(BBa_K823027)</font>]
+|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
-|Amp
+|[[File:LMU PromoterIconBC.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters]]
-|MLS
+|[[File:LMU Reporter.png|60px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters]]
-|lacA
+|[[File:Proteinaffinitytagbutton.png|50px|link=Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags]]
-|empty
-|pAX01
-|[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl ''et al.'']
 |-
 |}
+</div>
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